Immunohistochemical study of proliferation and apoptosis in the
dorsal root ganglia after supraspinal and infraspinal axotomy.

D 2000

Immunohistochemical study of proliferation and apoptosis in the dorsal root ganglia after supraspinal and infraspinal axotomy.

KLUSÁKOVÁ, Ilona, Petr DUBOVÝ a Ivana SVÍŽENSKÁ

Základní údaje

Originální název

Immunohistochemical study of proliferation and apoptosis in the dorsal root ganglia after supraspinal and infraspinal axotomy.

Autoři

KLUSÁKOVÁ, Ilona, Petr DUBOVÝ a Ivana SVÍŽENSKÁ

Vydání

Praha, Sborník lékařský, s. 14-14, Vol. 101, No. 4, 2000

Nakladatel

Karlova Univerzita

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

30000 3. Medical and Health Sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14110/00:00002597

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

spinal ganglia; TUNEL; apoptosis; axotomy

Štítky

apoptosis, axotomy, spinal ganglia, TUNEL

Změněno: 21. 12. 2000 10:04, MUDr. Ivana Hradilová Svíženská, CSc.

Anotace

V originále

Disconnection of the peripheral axonal branch from the target tissue results in changes of GFAP expression in the satellite cells of the dorsal root ganglia (DRG) and their proliferation The loss of DRG neurons was also described after the peripheral axotomy. No results are available about the reaction of DRG neurons and their satellite cells to the central axotomy. We have investigated proliferation and apoptosis in the adult rat DRG after central and peripheral axotomy. Female rats were operated for the central axotomy and peripheral axotomy. The animals were perfused through ascending aorta with phosphate-buffered saline followed Zamboni's fixative solution after 2, 4, and 6 weeks. Cryostat sections through affected and intact DRG were incubated for immunohistochemical identification of BrdU as well as apoptosis (TUNEL). Both peripheral and central axotomy stimulated proliferation of many satellite cells while apoptosis was observed in lower amount of the cells.

Návaznosti

GA309/00/0407, projekt VaV

Název: Celulární a extracelulární prostředí pro regeneraci axonů při kořenových a distálních lezích periferních nervů

Investor: Grantová agentura ČR, Celulární a extracelulární prostředí pro regeneraci axonů při kořenových a distálních lezích periferních nervů

Citovat

KLUSÁKOVÁ, Ilona, Petr DUBOVÝ a Ivana SVÍŽENSKÁ. Immunohistochemical study of proliferation and apoptosis in the dorsal root ganglia after supraspinal and infraspinal axotomy. In Sborník lékařský. Praha: Karlova Univerzita, 2000, s. 14. Vol. 101, No. 4.

@inproceedings{344847,
   author = {Klusáková, Ilona and Dubový, Petr and Svíženská, Ivana},
   address = {Praha},
   booktitle = {Sborník lékařský},
   keywords = {spinal ganglia; TUNEL; apoptosis; axotomy},
   language = {eng},
   location = {Praha},
   pages = {14-14},
   publisher = {Karlova Univerzita},
   title = {Immunohistochemical study of proliferation and apoptosis in the dorsal root ganglia after supraspinal and infraspinal axotomy.},
   year = {2000}
}

TY  - JOUR
ID  - 344847
AU  - Klusáková, Ilona - Dubový, Petr - Svíženská, Ivana
PY  - 2000
TI  - Immunohistochemical study of proliferation and apoptosis in the dorsal root ganglia after supraspinal and infraspinal axotomy.
VL  - Vol. 101, No. 4
PB  - Karlova Univerzita
CY  - Praha
KW  - spinal ganglia
KW  - TUNEL
KW  - apoptosis
KW  - axotomy
N2  - Disconnection of the peripheral axonal branch from the target tissue results in changes of GFAP expression in the satellite cells of the dorsal root ganglia (DRG) and their proliferation The loss of DRG neurons was also described after the peripheral axotomy. No results are available about the reaction of DRG neurons and their satellite cells to the central axotomy. We have investigated proliferation and apoptosis in the adult rat DRG after central and peripheral axotomy. Female rats were operated for the central axotomy and peripheral axotomy. The animals were perfused through ascending aorta with phosphate-buffered saline followed Zamboni's fixative solution after 2, 4, and 6 weeks. Cryostat sections through affected and intact DRG were incubated for immunohistochemical identification of BrdU as well as apoptosis (TUNEL). Both peripheral and central axotomy stimulated proliferation of many satellite cells while apoptosis was observed in lower amount of the cells.
ER  -

KLUSÁKOVÁ, Ilona, Petr DUBOVÝ a Ivana SVÍŽENSKÁ. Immunohistochemical study of proliferation and apoptosis in the dorsal root ganglia after supraspinal and infraspinal axotomy. In \textit{Sborník lékařský}. Praha: Karlova Univerzita, 2000, s.~14. Vol. 101, No. 4.

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