| VÉVODOVÁ, Jitka, Jaromír MAREK, Jan ZOUHAR, Břetislav BRZOBOHATÝ a X.-D. SU. Purification, Crystallization and Preliminary X-ray Analysis of a Maize Cytokinin-glucoside-specific b-Glucosidase. Acta Crystallogr., Sect.D. Dánsko, 2001, D57, č. 1, s. 140-142. ISSN 0907-4449. |
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@article{344939,
author = {Vévodová, Jitka and Marek, Jaromír and Zouhar, Jan and Brzobohatý, Břetislav and Su, X.andD.},
article_location = {Dánsko},
article_number = {1},
keywords = {beta-glucosidase; hydrolase; protein crystallography},
language = {eng},
issn = {0907-4449},
journal = {Acta Crystallogr., Sect.D},
title = {Purification, Crystallization and Preliminary X-ray Analysis of a Maize Cytokinin-glucoside-specific b-Glucosidase.},
volume = {D57},
year = {2001}
}
TY - JOUR
ID - 344939
AU - Vévodová, Jitka - Marek, Jaromír - Zouhar, Jan - Brzobohatý, Břetislav - Su, X.-D.
PY - 2001
TI - Purification, Crystallization and Preliminary X-ray Analysis of a Maize Cytokinin-glucoside-specific b-Glucosidase.
JF - Acta Crystallogr., Sect.D
VL - D57
IS - 1
SP - 140
EP - 140
SN - 09074449
KW - beta-glucosidase
KW - hydrolase
KW - protein crystallography
N2 - Zm-p60.1, a cytokinin-glucoside-specific beta-glucosidase from maize, is a key enzyme involved in plant development and growth. It has been over-expressed in soluble form from Escherichia coli with a His tag at its N-terminus. The recombinant protein has been purified and crystallized at room temperature using PEG 4000 as main precipitant. A few forms of crystals have been observed from very similar conditions. A flash-annealed monoclinic crystal diffracted to high resolution (beyond 2 A) with space group P21, and unit-cell parameters a = 55.66 A, b = 110.72 A, c = 72.94 A, b = 92.10 deg. The asymmetric unit is estimated and confirmed by molecular replacement solution to contain a Zm-p60.1 dimer, giving a crystal volume per protein mass (Vm) of 1.89 A3 Da-1 and a solvent content of 35%.
ER -
VÉVODOVÁ, Jitka, Jaromír MAREK, Jan ZOUHAR, Břetislav BRZOBOHATÝ a X.-D. SU. Purification, Crystallization and Preliminary X-ray Analysis of a Maize Cytokinin-glucoside-specific b-Glucosidase. \textit{Acta Crystallogr., Sect.D}. Dánsko, 2001, D57, č.~1, s.~140-142. ISSN~0907-4449.
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