| DUBOVÝ, Petr, Ivana SVÍŽENSKÁ, Ilona KLUSÁKOVÁ a Andrea ZÍTKOVÁ. Laminin molecules in freeze-treated nerve segments are associated with migrating Schwann cells that display the corresponding a61 integrin receptor. Glia. USA: Wiley-Liss, 2001, roč. 33, č. 1, s. 36-44. ISSN 0894-1491. |
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@article{346068,
author = {Dubový, Petr and Svíženská, Ivana and Klusáková, Ilona and Zítková, Andrea},
article_location = {USA},
article_number = {1},
keywords = {Acellular nerve graft; Basal lamina; Nerve regeneration},
language = {eng},
issn = {0894-1491},
journal = {Glia},
title = {Laminin molecules in freeze-treated nerve segments are associated with migrating Schwann cells that display the corresponding a61 integrin receptor},
volume = {33},
year = {2001}
}
TY - JOUR
ID - 346068
AU - Dubový, Petr - Svíženská, Ivana - Klusáková, Ilona - Zítková, Andrea
PY - 2001
TI - Laminin molecules in freeze-treated nerve segments are associated with migrating Schwann cells that display the corresponding a61 integrin receptor
JF - Glia
VL - 33
IS - 1
SP - 36
EP - 36
PB - Wiley-Liss
SN - 08941491
KW - Acellular nerve graft
KW - Basal lamina
KW - Nerve regeneration
N2 - The presence of laminin-1, and -2, as well as a6 and b1 integrin chains was detected by indirect immunohistochemistry in the sections through acellular nerve segments 7 and 14 days after the cryo-treatment. The position of basal laminae and Schwann cells was identified by immunostaining for collagen-IV and S-100 protein, respectively. The isolated cryo-treated segment without living Schwann cells (S-100-) did not display immunoreactivity for laminins and integrin's chains while the basal lamina position was verified through the whole segment by immunostaining for collagen-IV. The absence of immunostaining for laminin-1 and -2 in cryo-treated nerve segment was confirmed by Western blot analysis. A crucial diminution of laminin-1 and -2 in cryo-treated nerve segment of 10 mm length did not abolish the growth and maturation of axons. The greater part of nerve segment connected with the nerve stump displayed no immunohistochemical staining for S-100 corresponding with absence of Schwann cells. The border region of the nerve segment contained Schwann cells (S-100+) migrating from near freeze-undamaged part of the distal nerve stump. In addition to immunostaining for S-100 protein, the migrating Schwann cells displayed immunostaining for laminins (-1, and -2) and integrin chains (a6 and b1). The results indicate laminin molecules in the acellular nerve segments are related with the migrating Schwann cells. Both immunostaining for laminins and integrin chains suggests an autocrine and/or paracrine utilization of laminin molecules in the promotion of Schwann cell migration.
ER -
DUBOVÝ, Petr, Ivana SVÍŽENSKÁ, Ilona KLUSÁKOVÁ a Andrea ZÍTKOVÁ. Laminin molecules in freeze-treated nerve segments are associated with migrating Schwann cells that display the corresponding a61 integrin receptor. \textit{Glia}. USA: Wiley-Liss, 2001, roč.~33, č.~1, s.~36-44. ISSN~0894-1491.
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