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@article{346313,
author = {Kozubek, Michal and Matula, Petr},
article_location = {Oxford},
article_number = {3},
keywords = {chromatic aberration; fluorescence microscopy; fluorescent bead; image analysis},
language = {eng},
issn = {0022-2720},
journal = {Journal of Microscopy},
title = {An efficient algorithm for measurement and correction of chromatic aberrations in fluorescence microscopy},
url = {https://doi.org/10.1046/j.1365-2818.2000.00754.x},
volume = {200},
year = {2000}
}
TY - JOUR
ID - 346313
AU - Kozubek, Michal - Matula, Petr
PY - 2000
TI - An efficient algorithm for measurement and correction of chromatic aberrations in fluorescence microscopy
JF - Journal of Microscopy
VL - 200
IS - 3
SP - 206-217
EP - 206-217
PB - Blackwell Science
SN - 00222720
KW - chromatic aberration
KW - fluorescence microscopy
KW - fluorescent bead
KW - image analysis
UR - https://doi.org/10.1046/j.1365-2818.2000.00754.x
L2 - https://doi.org/10.1046/j.1365-2818.2000.00754.x
N2 - Even the best optical microscopes available on the market exhibit chromatic aberrations to some extent. In some types of study, chromatic aberrations of current optics cannot be neglected and a software correction is highly desirable. This paper describes a novel method of chromatic aberration measurement and software correction using sub-resolution bead imaging and computer image analysis. The method is quick, precise and enables the determination of both longitudinal and lateral chromatic aberrations. Correction function can be computed in about half an hour, including image acquisition. Using this approach, chromatic aberrations can be reduced to 10-20 nm laterally and 10-60 nm axially depending on the type of optical set-up. The method is especially suitable for fluorescence microscopy, where a limited number of wavelengths are observed.
ER -
KOZUBEK, Michal a Petr MATULA. An efficient algorithm for measurement and correction of chromatic aberrations in fluorescence microscopy. \textit{Journal of Microscopy}. Oxford: Blackwell Science, 2000, roč.~200, č.~3, s.~206-217. ISSN~0022-2720.
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