Electrochemical impedance spectroscopy of single-stranded and
double-helical polynucleotides

D 2000

Electrochemical impedance spectroscopy of single-stranded and double-helical polynucleotides

VETTERL, Vladimír; František JELEN; Viktor DRAŽAN; Luděk STRAŠÁKA; Stanislav HASOŇ et. al.

Základní údaje

Originální název

Electrochemical impedance spectroscopy of single-stranded and double-helical polynucleotides

Autoři

VETTERL, Vladimír; František JELEN; Viktor DRAŽAN; Luděk STRAŠÁKA; Stanislav HASOŇ a Jakub DVOŘÁK

Vydání

Praha, Book of Abstracts, J.Heyrovský Memorial Symposium, Praha, s. 5-5, 2000

Nakladatel

Ústav fyzikální chemie J.Heyrovského, AV ČR

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10610 Biophysics

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14330/00:00004092

Organizační jednotka

Fakulta informatiky

Klíčová slova anglicky

DNA adsorption; electrode double layer; impedance spectroscopy

Štítky

DNA adsorption, electrode double layer, impedance spectroscopy

Změněno: 30. 6. 2008 14:32, prof. RNDr. Vladimír Vetterl, DrSc.

Anotace

V originále

The dependence of the impedance of the electrode double layer on time and frequency around the potentials of the tensammetric peaks and around the potential of maximum adsorption of single-stranded and double-helical polyadenylic acid (poly A) and DNA was studied. At room temperature the single stranded poly A (pH 8) yields a high tensammetric peak at about -1.4 V vs.SCE. The peak is higher and narrower at low ionic strengths. The height of the peak decreases with increasing frequency at low ionic strengths while it is almost independent on the frequency at higher ionic strengths. With double-helical (native ) DNA only one desorption maximum is observed on the C-E curves, single-stranded (denatured) DNA yields two maxima. It was shown by Miller and later by Palecek that the more positive desorption peak at -1.15 V yielded both by the native and denatured DNA corresponds to the desorption of the sugar - phospate backbone. The more negative peak at -1.4 V which yields only denatured DNA corresponds to the more firmly adsorbed bases.

Návaznosti

GA203/00/P081, projekt VaV

Název: Adsorpce bází nukleových kyselin a jejich derivátů na elektrodách

GV204/97/K084, projekt VaV

Název: Elektrody modifikované nukleovými kyselinami a bílkovinami. Nové nástroje v biochemickém a biomedicínském výzkumu

Investor: Grantová agentura ČR, Elektrody modifikované nukleovými kyselinami a bílkovinami. Nové nástroje v biochemickém a biomedicínském výzkumu

IAA4004002, projekt VaV

Název: Struktura a interakce nukleových kyselin a polypeptidů na površích kovů

Investor: Akademie věd ČR, Struktura a interakce nukleových kyselin a polypeptidů na površích kovů

Citovat

VETTERL, Vladimír; František JELEN; Viktor DRAŽAN; Luděk STRAŠÁKA; Stanislav HASOŇ a Jakub DVOŘÁK. Electrochemical impedance spectroscopy of single-stranded and double-helical polynucleotides. In Book of Abstracts, J.Heyrovský Memorial Symposium, Praha. Praha: Ústav fyzikální chemie J.Heyrovského, AV ČR, 2000, s. 5.

@inproceedings{355871,
   author = {Vetterl, Vladimír and Jelen, František and Dražan, Viktor and Strašáka, Luděk and Hasoň, Stanislav and Dvořák, Jakub},
   address = {Praha},
   booktitle = {Book of Abstracts, J.Heyrovský Memorial Symposium, Praha},
   keywords = {DNA adsorption; electrode double layer; impedance spectroscopy},
   language = {eng},
   location = {Praha},
   pages = {5-5},
   publisher = {Ústav fyzikální chemie J.Heyrovského, AV ČR},
   title = {Electrochemical impedance spectroscopy of single-stranded and double-helical polynucleotides},
   year = {2000}
}

TY  - CONF
ID  - 355871
AU  - Vetterl, Vladimír - Jelen, František - Dražan, Viktor - Strašáka, Luděk - Hasoň, Stanislav - Dvořák, Jakub
PY  - 2000
TI  - Electrochemical impedance spectroscopy of single-stranded and double-helical polynucleotides
PB  - Ústav fyzikální chemie J.Heyrovského, AV ČR
CY  - Praha
KW  - DNA adsorption
KW  - electrode double layer
KW  - impedance spectroscopy
N2  - The dependence of the impedance of the electrode double layer on time and frequency around the potentials of the tensammetric peaks and around the potential of maximum adsorption of single-stranded and double-helical polyadenylic acid (poly A) and DNA was studied. At room temperature the single stranded poly A (pH 8) yields a high tensammetric peak at about -1.4 V vs.SCE. The peak is higher and narrower at low ionic strengths. The height of the peak decreases with increasing frequency at low ionic strengths while it is almost independent on the frequency at higher ionic strengths. With double-helical (native ) DNA only one desorption maximum is observed on the C-E curves, single-stranded (denatured) DNA yields two maxima. It was shown by Miller and later by Palecek that the more positive desorption peak at -1.15 V yielded both by the native and denatured DNA corresponds to the desorption of the sugar - phospate backbone. The more negative peak at -1.4 V which yields only denatured DNA corresponds to the more firmly adsorbed bases.
ER  -

VETTERL, Vladimír; František JELEN; Viktor DRAŽAN; Luděk STRAŠÁKA; Stanislav HASOŇ a Jakub DVOŘÁK. Electrochemical impedance spectroscopy of single-stranded and double-helical polynucleotides. In \textit{Book of Abstracts, J.Heyrovský Memorial Symposium, Praha}. Praha: Ústav fyzikální chemie J.Heyrovského, AV ČR, 2000, s.~5.

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