Telomerase Activity and Expresion and Telomere Analysis in Situ in the Course of Treatment of Childhood Leukemias

MALÁSKA, Jan, Marie SKLENIČKOVÁ, Kateřina KREJČÍ, Lenka FAJKUSOVÁ, Milan BAJER, Hana HRSTKOVÁ and Jiří FAJKUS. Telomerase Activity and Expresion and Telomere Analysis in Situ in the Course of Treatment of Childhood Leukemias (Telomerase Activity and Expression and Telomere Analysis in Situ in the Course of Treatment of Childhood Leukemias). Blood Cells, Molecules and Diseases. Orlando, Florida: Academic Press, 2000, vol. 26, No 5, p. 534-539. ISSN 1079-9796.

Basic information

• Original name: Telomerase Activity and Expresion and Telomere Analysis in Situ in the Course of Treatment of Childhood Leukemias
• Authors: MALÁSKA, Jan (203 Czech Republic), Marie SKLENIČKOVÁ (203 Czech Republic), Kateřina KREJČÍ, Lenka FAJKUSOVÁ (203 Czech Republic), Milan BAJER (203 Czech Republic), Hana HRSTKOVÁ (203 Czech Republic) and Jiří FAJKUS (203 Czech Republic, guarantor).
• Edition: Blood Cells, Molecules and Diseases, Orlando, Florida, Academic Press, 2000, 1079-9796.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 30209 Paediatrics
• Country of publisher: United States of America
• Confidentiality degree: is not subject to a state or trade secret
• Impact factor: Impact factor: 2.897
• RIV identification code: RIV/00216224:14310/00:00004094
• Organization unit: Faculty of Science
• UT WoS: 000165787500158
• Keywords in English: acute leukemias; telomerase; in situ analysis of telomeres; chemotherapy; predictive factors
• Tags: acute leukemias, chemotherapy, predictive factors, Telomerase

Abstract

Samples of blood and marrow from children with leukemia were assayed for telomerase activity nd expression on the day of diagnosis and during the course of chemotherapy. A strong correlation between either variables and clinical response was observed in most patients. A unique case was observed in which telomerase activity was only moderately increased on diagnosis, it gradually increased in the course of therapy, and a subsequent decrease occurred only after application of intensified therapy. This patient did not respond to therapy, his disease progressed, and he finally died during intensified therapy. In another patient, analysis of telomere lengths using dideoxy-PRINS revealed a single telomere expansion on a long arm of chromosome 4, uggesting involvement of a telomerase-independent mechanism of telomere elongation.

Links

• GA301/99/0045, research and development project: Name: Dynamika telomer u vybraných typů solidních maligních onemocnění

Investor: Czech Science Foundation, Telomere dynamics in selected types of solid malignant diseases

• MSM 143100008, plan (intention): Name: Genomy a jejich funkce

Investor: Ministry of Education, Youth and Sports of the CR, Genomes and their functions

• VS97032, research and development project: Name: Analýza biologicky významných molekulárních komplexů

Investor: Ministry of Education, Youth and Sports of the CR, Analysis of biologically important molecular complexes