Use of GBS media for rapid detection of group B streptococci in vaginal and rectal swabs from women in labor.

VOTAVA, Miroslav, Renata TEJKALOVÁ, Marie DRÁBKOVÁ, Vít UNZEITIG a Ilja BRAVENY. Use of GBS media for rapid detection of group B streptococci in vaginal and rectal swabs from women in labor. Europ. J. Clin. Microbiol. Infect. Dis. Heidelberg: Springer-Verlag, 2001, roč. 20, č. 2, s. 120-122. ISSN 0934-9723.

Základní údaje

• Originální název: Use of GBS media for rapid detection of group B streptococci in vaginal and rectal swabs from women in labor.
• Název anglicky: Use of GBS media for rapid detection of group B streptococci in vaginal and rectal swabs from women in labor.
• Autoři: VOTAVA, Miroslav, Renata TEJKALOVÁ, Marie DRÁBKOVÁ, Vít UNZEITIG a Ilja BRAVENY.
• Vydání: Europ. J. Clin. Microbiol. Infect. Dis. Heidelberg, Springer-Verlag, 2001, 0934-9723.

Další údaje

• Typ výsledku: Článek v odborném periodiku
• Utajení: není předmětem státního či obchodního tajemství
• Impakt faktor: Impact factor: 1.386
• Organizační jednotka: Lékařská fakulta
• UT WoS: 000167531600008
• Klíčová slova anglicky: group B streptococci - rapid detection - women in labor - vaginal swabs - rectal swabs - GBS media - culture od GBS - naternal GBS colonization - neonatal GBS colonization

Anotace

In order to evaluate the differences in efficacy, three methods were used to detect GBS in women in labor. The recommended method for detecting GBS carriage in pregnant women is to culture vaginal and anorectal swabs in a selective broth medium and to subculture them onto blood agar. The method was compared with the use of GBS agar and GBS broth, both of which produce an orange pigment in response to GBS strains. A total of 319 women in labor were screened. Among the 638 specimens tested, 134 (21 %) were positive in the selective Todd-Hewitt broth subcultured onto sheep blood agar, 133 (20.8 %)were positive on the GBS agar and 126 (19.7 %) were positive in the GBS broth. Altogether, 89 (27.9 %) women in labor were found to be colonized with GBS; 87 (97.8 %) of them were identified as carriers using the Todd-Hewitt broth, 87 (97.8 %) with the GBS agar and 86 (96.6 %) with the GBS broth. These results indicate that both GBS agar and GBS broth are reliable methods that can be used to screen for maternat and neonatal GBS colonization.

Anotace česky

In order to evaluate the differences in efficacy, three methods were used to detect GBS in women in labor. The recommended method for detecting GBS carriage in pregnant women is to culture vaginal and anorectal swabs in a selective broth medium and to subculture them onto blood agar. The method was compared with the use of GBS agar and GBS broth, both of which produce an orange pigment in response to GBS strains. A total of 319 women in labor were screened. Among the 638 specimens tested, 134 (21 %) were positive in the selective Todd-Hewitt broth subcultured onto sheep blood agar, 133 (20.8 %)were positive on the GBS agar and 126 (19.7 %) were positive in the GBS broth. Altogether, 89 (27.9 %) women in labor were found to be colonized with GBS; 87 (97.8 %) of them were identified as carriers using the Todd-Hewitt broth, 87 (97.8 %) with the GBS agar and 86 (96.6 %) with the GBS broth. These results indicate that both GBS agar and GBS broth are reliable methods that can be used to screen for maternat and neonatal GBS colonization.

Anotace anglicky

In order to evaluate the differences in efficacy, three methods were used to detect GBS in women in labor. The recommended method for detecting GBS carriage in pregnant women is to culture vaginal and anorectal swabs in a selective broth medium and to subculture them onto blood agar. The method was compared with the use of GBS agar and GBS broth, both of which produce an orange pigment in response to GBS strains. A total of 319 women in labor were screened. Among the 638 specimens tested, 134 (21 %) were positive in the selective Todd-Hewitt broth subcultured onto sheep blood agar, 133 (20.8 %)were positive on the GBS agar and 126 (19.7 %) were positive in the GBS broth. Altogether, 89 (27.9 %) women in labor were found to be colonized with GBS; 87 (97.8 %) of them were identified as carriers using the Todd-Hewitt broth, 87 (97.8 %) with the GBS agar and 86 (96.6 %) with the GBS broth. These results indicate that both GBS agar and GBS broth are reliable methods that can be used to screen for maternat and neonatal GBS colonization.