IMMUNOHISTOCHEMICAL DIFFERENCES IN THE ECM CONTENT ALONGSIDE
AFFERENT AND MOTOR AXONS IN RELATION TO THE POSITION OF THE
DORSAL ROOT GANGLION

D 2000

IMMUNOHISTOCHEMICAL DIFFERENCES IN THE ECM CONTENT ALONGSIDE AFFERENT AND MOTOR AXONS IN RELATION TO THE POSITION OF THE DORSAL ROOT GANGLION

SVÍŽENSKÁ, Ivana; Ilona KLUSÁKOVÁ; Nabil Ahmed GHALIB a Petr DUBOVÝ

Základní údaje

Originální název

IMMUNOHISTOCHEMICAL DIFFERENCES IN THE ECM CONTENT ALONGSIDE AFFERENT AND MOTOR AXONS IN RELATION TO THE POSITION OF THE DORSAL ROOT GANGLION

Autoři

SVÍŽENSKÁ, Ivana; Ilona KLUSÁKOVÁ; Nabil Ahmed GHALIB a Petr DUBOVÝ

Vydání

Brno, 37th Proceedings of Czech Society of Histochemistry and Cytochemistry, s. 48-48, 37th Proceeding of Czech Society of Histochemistry, 2000

Nakladatel

MU Brno

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

30000 3. Medical and Health Sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/00:00003131

Organizační jednotka

Lékařská fakulta

Změněno: 19. 3. 2001 17:11, prof. RNDr. Petr Dubový, CSc.

Anotace

V originále

To reveal different microenvironmental conditions for motor and afferent axons we have analyzed immunohistochemically some ECM components and adhesion molecules (laminin-2, -3, fibronectin, thrombospondin, tenascin, chondroitin sulfate, and HNK-1) in the spinal roots and branches of the spinal nerve.Our results revealed no differences in the fibronectin immunofluorescence between dorsal and ventral roots as well as dorsal and ventral branches of the spinal nerve. There were also no differences between ventral and dorsal branches in the thrombospondin occurrence peripheral to DRG, and no differences were found between ventral and dorsal root contents of both laminin-2 and -3 central to the DRG. A higher immunofluorescence staining for HNK-1 and CS-56 was proved in the dorsal root as well as dorsal branch of the spinal nerve in contrast to the ventral root and branch. The greater amount of thrombospondin and tenascin molecules was disclosed in the dorsal roots than ventral ones, while higher immunofluorescence staining for laminin-2 and -3 was found in the dorsal in contrast to ventral branches of the peripheral nerves. On the other hand, tenascin molecules were more abundant in the ventral branches of the spinal nerve than the dorsal ones. The results of quantitative immunofluorescence analysis suggest some differences in the content of ECM along the afferent and motor axons that could be involved in the origin of different conditions for the axon regrowth after injury of the dorsal and ventral roots as well as the spinal nerves.

Návaznosti

GA309/00/0407, projekt VaV

Název: Celulární a extracelulární prostředí pro regeneraci axonů při kořenových a distálních lezích periferních nervů

Investor: Grantová agentura ČR, Celulární a extracelulární prostředí pro regeneraci axonů při kořenových a distálních lezích periferních nervů

Citovat

SVÍŽENSKÁ, Ivana; Ilona KLUSÁKOVÁ; Nabil Ahmed GHALIB a Petr DUBOVÝ. IMMUNOHISTOCHEMICAL DIFFERENCES IN THE ECM CONTENT ALONGSIDE AFFERENT AND MOTOR AXONS IN RELATION TO THE POSITION OF THE DORSAL ROOT GANGLION. In 37th Proceedings of Czech Society of Histochemistry and Cytochemistry. Brno: MU Brno, 2000, s. 48. 37th Proceeding of Czech Society of Histochemistry.

@inproceedings{358835,
   author = {Svíženská, Ivana and Klusáková, Ilona and Ghalib, Nabil Ahmed and Dubový, Petr},
   address = {Brno},
   booktitle = {37th Proceedings of Czech Society of Histochemistry and Cytochemistry},
   language = {eng},
   location = {Brno},
   pages = {48-48},
   publisher = {MU Brno},
   title = {IMMUNOHISTOCHEMICAL DIFFERENCES IN THE ECM CONTENT ALONGSIDE AFFERENT AND MOTOR AXONS IN RELATION TO THE POSITION OF THE DORSAL ROOT GANGLION},
   year = {2000}
}

TY  - CONF
ID  - 358835
AU  - Svíženská, Ivana - Klusáková, Ilona - Ghalib, Nabil Ahmed - Dubový, Petr
PY  - 2000
TI  - IMMUNOHISTOCHEMICAL DIFFERENCES IN THE ECM CONTENT ALONGSIDE AFFERENT AND MOTOR AXONS IN RELATION TO THE POSITION OF THE DORSAL ROOT GANGLION
VL  - 37th Proceeding of Czech Society of Histochemistry
PB  - MU Brno
CY  - Brno
N2  - To reveal different microenvironmental conditions for motor and afferent axons we have analyzed immunohistochemically some ECM components and adhesion molecules (laminin-2, -3, fibronectin, thrombospondin, tenascin, chondroitin sulfate, and HNK-1) in the spinal roots and branches of the spinal nerve.Our results revealed no differences in the fibronectin immunofluorescence between dorsal and ventral roots as well as dorsal and ventral branches of the spinal nerve. There were also no differences between ventral and dorsal branches in the thrombospondin occurrence peripheral to DRG, and no differences were found between ventral and dorsal root contents of both laminin-2 and -3 central to the DRG. A higher immunofluorescence staining for HNK-1 and CS-56 was proved in the dorsal root as well as dorsal branch of the spinal nerve in contrast to the ventral root and branch. The greater amount of thrombospondin and tenascin molecules was disclosed in the dorsal roots than ventral ones, while higher immunofluorescence staining for laminin-2 and -3 was found in the dorsal in contrast to ventral branches of the peripheral nerves. On the other hand, tenascin molecules were more abundant in the ventral branches of the spinal nerve than the dorsal ones. The results of quantitative immunofluorescence analysis suggest some differences in the content of ECM along the afferent and motor axons that could be involved in the origin of different conditions for the axon regrowth after injury of the dorsal and ventral roots as well as the spinal nerves.
ER  -

SVÍŽENSKÁ, Ivana; Ilona KLUSÁKOVÁ; Nabil Ahmed GHALIB a Petr DUBOVÝ. IMMUNOHISTOCHEMICAL DIFFERENCES IN THE ECM CONTENT ALONGSIDE AFFERENT AND MOTOR AXONS IN RELATION TO THE POSITION OF THE DORSAL ROOT GANGLION. In \textit{37th Proceedings of Czech Society of Histochemistry and Cytochemistry}. Brno: MU Brno, 2000, s.~48. 37th Proceeding of Czech Society of Histochemistry.

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