Postreceptor effect of antidepressants on C6 glioma cells and
NK lymphocytes

J 1999

Postreceptor effect of antidepressants on C6 glioma cells and NK lymphocytes

KOVÁŘŮ, Hana; Anna FIŠEROVÁ; Alena ŠPANOVÁ; Eva MATALOVÁ; Věra LISÁ et al.

Základní údaje

Originální název

Postreceptor effect of antidepressants on C6 glioma cells and NK lymphocytes

Autoři

KOVÁŘŮ, Hana; Anna FIŠEROVÁ; Alena ŠPANOVÁ; Eva MATALOVÁ a Věra LISÁ

Vydání

Physiological Research, Praha, Institute of Physiology, CAV, CR, 1999, 082-8408

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30105 Physiology

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/99:00004115

Organizační jednotka

Přírodovědecká fakulta

Změněno: 29. 3. 2001 13:10, doc. RNDr. Alena Španová, CSc.

Anotace

V originále

We studied antidepressant (AD) evoked cell signalling alterations in relationship to heterotrimeric GTP-binding (G) proteins, effector phospholipase C (PLC) and apoptosis. We used rat C6 glioma cell line, C6 glioma cells transfected (t) with pig thymus lymphocyte markers (MHCII, CD4 etc.)and natural killer (NK) lymphocytes. We analysed postreceptor effects of fluoxetine, FLU and sertraline, SER, specific serotonin reuptake inhibitors (SSR1). We estimated acute effects (20-24 h) and AD on Galfa subunits using ELISA and western immunoblotting with our antibodies. We demonstrated FLU effect on decreased level of Galfa q/11 in both C6 glioma and NK cells, in contrast to FLU induced changes of Galfas and Galfa(i1,2) in (t)C6 glioma cells. In C6 glioma cells, FLU induced Galga q/11 subunit translocation from membrane into cytosol and lower IP3 level, second messenger released by PLC and subsequent apoptotic events were observed. On the other hand SER effects on C6 glioma cells were linked to Galfys mainly without both IP3 changes and apoptosis.

Návaznosti

GA310/98/0347, projekt VaV

Název: Úloha dopaminu a prolactinu v průběhu nádorového růstu a imunomodulační účinky dopaminergních námelových alkaloidů.

Citovat

KOVÁŘŮ, Hana; Anna FIŠEROVÁ; Alena ŠPANOVÁ; Eva MATALOVÁ a Věra LISÁ. Postreceptor effect of antidepressants on C6 glioma cells and NK lymphocytes. Physiological Research. Praha: Institute of Physiology, CAV, CR, 1999, roč. 1999, č. 48, s. 87. ISSN 082-8408.

@article{358992,
   author = {Kovářů, Hana and Fišerová, Anna and Španová, Alena and Matalová, Eva and Lisá, Věra},
   article_location = {Praha},
   article_number = {48},
   language = {eng},
   issn = {082-8408},
   journal = {Physiological Research},
   title = {Postreceptor effect of antidepressants on C6 glioma cells and NK lymphocytes},
   volume = {1999},
   year = {1999}
}

TY  - JOUR
ID  - 358992
AU  - Kovářů, Hana - Fišerová, Anna - Španová, Alena - Matalová, Eva - Lisá, Věra
PY  - 1999
TI  - Postreceptor effect of antidepressants on C6 glioma cells and NK lymphocytes
JF  - Physiological Research
VL  - 1999
IS  - 48
SP  - 87
EP  - 87
PB  - Institute of Physiology, CAV, CR
SN  - 0828408
N2  - We studied antidepressant (AD) evoked cell signalling alterations in relationship to heterotrimeric GTP-binding (G) proteins, effector phospholipase C (PLC) and apoptosis. We used rat C6 glioma cell line, C6 glioma cells transfected (t) with pig thymus lymphocyte markers (MHCII, CD4 etc.)and natural killer (NK) lymphocytes. We analysed postreceptor effects of fluoxetine, FLU and sertraline, SER, specific serotonin reuptake inhibitors (SSR1). We estimated acute effects (20-24 h) and AD on Galfa subunits using ELISA and western immunoblotting with our antibodies. We demonstrated FLU effect on decreased level of Galfa q/11 in both C6 glioma and NK cells, in contrast to FLU induced changes of Galfas and Galfa(i1,2) in (t)C6 glioma cells. In C6 glioma cells, FLU induced Galga q/11 subunit translocation from membrane into cytosol and lower IP3 level, second messenger released by PLC and subsequent apoptotic events were observed. On the other hand SER effects on C6 glioma cells were linked to Galfys mainly without both IP3 changes and apoptosis.
ER  -

KOVÁŘŮ, Hana; Anna FIŠEROVÁ; Alena ŠPANOVÁ; Eva MATALOVÁ a Věra LISÁ. Postreceptor effect of antidepressants on C6 glioma cells and NK lymphocytes. \textit{Physiological Research}. Praha: Institute of Physiology, CAV, CR, 1999, roč.~1999, č.~48, s.~87. ISSN~082-8408.

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