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@article{359452,
author = {Glatz, Zdeněk and Nováková, Soňa and Štěrbová, Hana},
article_location = {Netherlands},
article_number = {(1+2)},
keywords = {Capillary electrophoresis; Rhodenase; Thiocyanate; Biuological Fluids},
language = {eng},
issn = {0021-9606},
journal = {Journal of Chromatography A},
title = {Analysis of Thiocyanate in Biological Fluids by Capillary Zone Electrophoresis},
volume = {916},
year = {2001}
}
TY - JOUR
ID - 359452
AU - Glatz, Zdeněk - Nováková, Soňa - Štěrbová, Hana
PY - 2001
TI - Analysis of Thiocyanate in Biological Fluids by Capillary Zone Electrophoresis
JF - Journal of Chromatography A
VL - 916
IS - (1+2)
SP - 273
EP - 273
PB - Elsevier
SN - 00219606
KW - Capillary electrophoresis
KW - Rhodenase
KW - Thiocyanate
KW - Biuological Fluids
N2 - A new sensitive and simple method has been developed for the determination of thiocyanate in human serum, urine and saliva. The determinations were performed in a 75 mm fused silica capillary (64.5 cm length, 56 cm effective length) using 0.1 M b-alanine - HCl (pH 3.50) as a background electrolyte, separation voltage 18 kV (negative polarity), temperature of capillary 25 oC and direct detection at 200 nm. Serum samples were 10-times diluted with deionised water and deproteinised with acetonitrile in the ratio 1 : 2. Urine and saliva samples need only 20-fold dilution with deionised water. The proposed method was successfully applied to the determination of thiocyanate in various human serum, saliva and urine samples.
ER -
GLATZ, Zdeněk, Soňa NOVÁKOVÁ a Hana ŠTĚRBOVÁ. Analysis of Thiocyanate in Biological Fluids by Capillary Zone Electrophoresis. \textit{Journal of Chromatography A}. Netherlands: Elsevier, 2001, roč.~916, (1+2), s.~273-277. ISSN~0021-9606.
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