Polymorphisms 1704G/T and 2184A/G in the RAGE gene are
associated with antioxidant status

J 2001

Polymorphisms 1704G/T and 2184A/G in the RAGE gene are associated with antioxidant status

KAŇKOVÁ, Kateřina, Ivana MÁROVÁ, Jiří ZÁHEJSKÝ, Jan MUŽÍK, Andrea STEJSKALOVÁ et. al.

Základní údaje

Originální název

Polymorphisms 1704G/T and 2184A/G in the RAGE gene are associated with antioxidant status

Název česky

Polymorfizmy 1704G/T a 2184A/G v RAGE genu jsou asociovany s antioxidacnimi parametry

Autoři

KAŇKOVÁ, Kateřina (203 Česká republika), Ivana MÁROVÁ (203 Česká republika), Jiří ZÁHEJSKÝ (203 Česká republika), Jan MUŽÍK (203 Česká republika), Andrea STEJSKALOVÁ (203 Česká republika), Vladimír ZNOJIL (203 Česká republika) a Jiří VÁCHA (203 Česká republika)

Vydání

Metabolism: Clinical and Experimental, USA, W. B. Saunders Company, 2001, 0026-0495

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.931

Kód RIV

RIV/00216224:14110/01:00004172

Organizační jednotka

Lékařská fakulta

UT WoS

000171538800006

Změněno: 22. 2. 2005 09:40, prof. MUDr. Kateřina Kaňková, Ph.D.

Anotace

ORIG CZ

V originále

BACKGROUND: The formation of advanced glycation end products (AGEs) and oxidative stress are supposed to play an important role in the development of diabetic late complications. AGEs can bind to several binding sites including Receptor of Advanced Glycation End products (RAGE). AGE-RAGE interaction results in free radical generation. The aim of the present study was to investigate the impact of previously described polymorphisms in the RAGE gene (G82S, 1704G/T, 2184A/G and 2245G/A) on the glycoxidation status in NIDDM. METHODS: A total of 371 unrelated Caucasian subjects were enrolled in the study. The NIDDM group comprised 202 subjects, the presence of late diabetic complications in five particular localizations was expressed as an index (Icompl). The non-diabetic group comprised 169 subjects. Glycated hemoglobin (HbA1c), glycated stratum corneum proteins (Amadori, AGE), total carotenoids, a- and b-carotene, g-tocopherol, lutein, lycopene and a-tocopherol were measured in each subject. RESULTS: Statistically significant differences in allele frequencies between the NIDDM and the non-diabetic groups were observed for the G82S and 2245G/A polymorphisms (P=0.047 and 0.032, resp.). HbA1c, Amadori and AGE did not reveal any significant association with any of the polymorphisms analyzed. However, significant differences between subjects bearing "wild type majority" genotypes 1704GG+2184AA and subjects with "mutated" genotypes were found for total carotenoids (P=0.001), a-carotene (P=0.046), b-carotene (P=0.028), lutein (P=0.001), lycopene (P=0.006) and a-tocopherol (P=0.047). Icompl significantly correlated with the plasma levels of all antioxidants (all P<0.01), while no correlation of Icompl with glycation variables was observed. CONCLUSIONS: The newly identified intron polymorphisms in the RAGE gene were proved to be associated with the antioxidant status in NIDDM subjects. The extent of diabetic vascular disease is related to the plasma levels of antioxidants.

Česky

Zjistili jsme, ze polymorfizmy 1704G/T a 2184A/G v RAGE genu jsou asociovany s antioxidacnimi parametry.

Návaznosti

MSM 141100002, záměr

Název: Molekulární patofyziologie multigenních chorob

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Molekulární patofyziologie multigenních chorob

VS96097, projekt VaV

Název: Molekulární patofyziologie vybraných "civilizačních", multigenně podmíněných chorob

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Molekulární patofyziologie vybraných "civilizačních", multigenně podmíněných chorob

Citovat

KAŇKOVÁ, Kateřina, Ivana MÁROVÁ, Jiří ZÁHEJSKÝ, Jan MUŽÍK, Andrea STEJSKALOVÁ, Vladimír ZNOJIL a Jiří VÁCHA. Polymorphisms 1704G/T and 2184A/G in the RAGE gene are associated with antioxidant status. Metabolism: Clinical and Experimental. USA: W. B. Saunders Company, 2001, roč. 50, č. 10, s. 1152-1160. ISSN 0026-0495.

@article{361433,
   author = {Kaňková, Kateřina and Márová, Ivana and Záhejský, Jiří and Mužík, Jan and Stejskalová, Andrea and Znojil, Vladimír and Vácha, Jiří},
   article_location = {USA},
   article_number = {10},
   language = {eng},
   issn = {0026-0495},
   journal = {Metabolism: Clinical and Experimental},
   title = {Polymorphisms 1704G/T and 2184A/G in the RAGE gene are associated with antioxidant status},
   volume = {50},
   year = {2001}
}

TY  - JOUR
ID  - 361433
AU  - Kaňková, Kateřina - Márová, Ivana - Záhejský, Jiří - Mužík, Jan - Stejskalová, Andrea - Znojil, Vladimír - Vácha, Jiří
PY  - 2001
TI  - Polymorphisms 1704G/T and 2184A/G in the RAGE gene are associated with antioxidant status
JF  - Metabolism: Clinical and Experimental
VL  - 50
IS  - 10
SP  - 1152
EP  - 1152
PB  - W. B. Saunders Company
SN  - 00260495
N2  - BACKGROUND: The formation of advanced glycation end products (AGEs) and oxidative stress are supposed to play an important role in the development of diabetic late complications. AGEs can bind to several binding sites including Receptor of Advanced Glycation End products (RAGE). AGE-RAGE interaction results in free radical generation. The aim of the present study was to investigate the impact of previously described polymorphisms in the RAGE gene (G82S, 1704G/T, 2184A/G and 2245G/A) on the glycoxidation status in NIDDM. METHODS: A total of 371 unrelated Caucasian subjects were enrolled in the study. The NIDDM group comprised 202 subjects, the presence of late diabetic complications in five particular localizations was expressed as an index (Icompl). The non-diabetic group comprised 169 subjects. Glycated hemoglobin (HbA1c), glycated stratum corneum proteins (Amadori, AGE), total carotenoids, a- and b-carotene, g-tocopherol, lutein, lycopene and a-tocopherol were measured in each subject. RESULTS: Statistically significant differences in allele frequencies between the NIDDM and the non-diabetic groups were observed for the G82S and 2245G/A polymorphisms (P=0.047 and 0.032, resp.). HbA1c, Amadori and AGE did not reveal any significant association with any of the polymorphisms analyzed. However, significant differences between subjects bearing "wild type majority" genotypes 1704GG+2184AA and subjects with "mutated" genotypes were found for total carotenoids (P=0.001), a-carotene (P=0.046), b-carotene (P=0.028), lutein (P=0.001), lycopene (P=0.006) and a-tocopherol (P=0.047). Icompl significantly correlated with the plasma levels of all antioxidants (all P<0.01), while no correlation of Icompl with glycation variables was observed. CONCLUSIONS: The newly identified intron polymorphisms in the RAGE gene were proved to be associated with the antioxidant status in NIDDM subjects. The extent of diabetic vascular disease is related to the plasma levels of antioxidants.
ER  -

KAŇKOVÁ, Kateřina, Ivana MÁROVÁ, Jiří ZÁHEJSKÝ, Jan MUŽÍK, Andrea STEJSKALOVÁ, Vladimír ZNOJIL a Jiří VÁCHA. Polymorphisms 1704G/T and 2184A/G in the RAGE gene are associated with antioxidant status. \textit{Metabolism: Clinical and Experimental}. USA: W. B. Saunders Company, 2001, roč.~50, č.~10, s.~1152-1160. ISSN~0026-0495.

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