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@article{362372,
author = {Jirsová, Pavla and Kozubek, Stanislav and Bártová, Eva and Kozubek, Michal and Lukášová, Emilie and Gaňová, Alena and Koutná, Irena and Skalníková, Magdalena},
article_number = {2},
keywords = {DOUBLE-STRAND BREAKS; SACCHAROMYCES-CEREVISIAE; HOMOLOGOUS RECOMBINATION; CHROMOSOME},
language = {eng},
issn = {0033-7587},
journal = {Radiation Research},
title = {Spatial distribution of selected genetic loci in nuclei of human leukemia cells after irradiation},
url = {http://www.fi.muni.cz/lom/abstracts/lom011.shtml},
volume = {155},
year = {2001}
}
TY - JOUR
ID - 362372
AU - Jirsová, Pavla - Kozubek, Stanislav - Bártová, Eva - Kozubek, Michal - Lukášová, Emilie - Gaňová, Alena - Koutná, Irena - Skalníková, Magdalena
PY - 2001
TI - Spatial distribution of selected genetic loci in nuclei of human leukemia cells after irradiation
JF - Radiation Research
VL - 155
IS - 2
SP - 311
EP - 311
SN - 00337587
KW - DOUBLE-STRAND BREAKS
KW - SACCHAROMYCES-CEREVISIAE
KW - HOMOLOGOUS RECOMBINATION
KW - CHROMOSOME
UR - http://www.fi.muni.cz/lom/abstracts/lom011.shtml
N2 - Fluorescence in situ hybridization (FISH) combined with high-resolution cytometry was used to determine the topographic characteristics of the centromeric heterochromatin (of the chromosomes 6, 8, 9, 17) and the tumor suppressor gene TP53 (which is located on chromosome 17) in cells of the human leukemia cell lines ML-1 and U937. Analysis was performed on cells that were either untreated or irradiated with gamma rays and incubated for different intervals after exposure. Compared to untreated cells, homologous centromeres and the TP53 genes were found closer to each other and also closer to the nuclear center 2 h after irradiation. The spatial relationship between genetic elements returned to that of the unirradiated controls during the next 2-3 h. Statistical evaluation of our experimental results shows that homologous centromeres and the homologous genes are positioned closer to each other 2 h after irradiation because they are localized closer to the center of the nucleus (probably due to more pronounced decondensation of the chromatin related to repair), This radial movement of genetic loci, however, is not connected,vith repair of DSBs by processes involving homologous recombination, because the angular distribution of homologous sequences remains random after irradiation.
ER -
JIRSOVÁ, Pavla, Stanislav KOZUBEK, Eva BÁRTOVÁ, Michal KOZUBEK, Emilie LUKÁŠOVÁ, Alena GAŇOVÁ, Irena KOUTNÁ a Magdalena SKALNÍKOVÁ. Spatial distribution of selected genetic loci in nuclei of human leukemia cells after irradiation. \textit{Radiation Research}. 2001, roč.~155, č.~2, s.~311-319. ISSN~0033-7587.
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