Biophysical analysis of natural, double-helical DNA modified by
anticancer heterocyclic complexes of ruthenium(III) in
cell-free media

J 2001

Biophysical analysis of natural, double-helical DNA modified by anticancer heterocyclic complexes of ruthenium(III) in cell-free media

MALINA, Jaroslav, Olga NOVÁKOVÁ, Bernhard K. KEPPLER, Enzo ALESSIO, Viktor BRABEC et. al.

Základní údaje

Originální název

Biophysical analysis of natural, double-helical DNA modified by anticancer heterocyclic complexes of ruthenium(III) in cell-free media

Autoři

MALINA, Jaroslav, Olga NOVÁKOVÁ, Bernhard K. KEPPLER, Enzo ALESSIO a Viktor BRABEC

Vydání

Journal of Biological Inorganic Chemistry, germany, Springer-Verlag, 2001, 0949-368

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10610 Biophysics

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/01:00004215

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

DNA; ruthenium; cytostatika; interakce

Štítky

cytostatika, DNA, interakce, ruthenium

Změněno: 25. 5. 2001 15:15, prof. RNDr. Viktor Brabec, DrSc.

Anotace

V originále

Modifications of natural DNA by three anticancer heterocyclic ruthenium(III) compounds were studied by methods of molecular biophysics. These methods include: DNA binding studies using atomic absorption spectrophotometry, inhibition of restriction endonucleases, mapping of DNA adducts by transcription assay, interstrand cross-linking employing gel electrophoresis under denaturing conditions, DNA unwinding studied by gel electrophoresis, circular dichroism analysis of B to Z transition in DNA and DNA melting curves measured by absorption spectrophotometry. It has been suggested that the altered DNA binding mode of ruthenium compounds in comparison with cisplatin might be an important factor responsible for altered cytostatic activity of this class of ruthenium compounds in tumor cells.

Návaznosti

GA305/99/0695, projekt VaV

Název: Ovlivnění konformace DNA protinádorově účinnými komplexy kovů. Vztah k vývoji nových cytostatik.

Citovat

MALINA, Jaroslav, Olga NOVÁKOVÁ, Bernhard K. KEPPLER, Enzo ALESSIO a Viktor BRABEC. Biophysical analysis of natural, double-helical DNA modified by anticancer heterocyclic complexes of ruthenium(III) in cell-free media. Journal of Biological Inorganic Chemistry. germany: Springer-Verlag, 2001, roč. 6, č. 1, s. 435-445. ISSN 0949-368.

@article{362477,
   author = {Malina, Jaroslav and Nováková, Olga and Keppler, Bernhard K. and Alessio, Enzo and Brabec, Viktor},
   article_location = {germany},
   article_number = {1},
   keywords = {DNA; ruthenium; cytostatika; interakce},
   language = {eng},
   issn = {0949-368},
   journal = {Journal of Biological Inorganic Chemistry},
   title = {Biophysical analysis of natural, double-helical DNA modified by anticancer heterocyclic complexes of ruthenium(III) in cell-free media},
   volume = {6},
   year = {2001}
}

TY  - JOUR
ID  - 362477
AU  - Malina, Jaroslav - Nováková, Olga - Keppler, Bernhard K. - Alessio, Enzo - Brabec, Viktor
PY  - 2001
TI  - Biophysical analysis of natural, double-helical DNA modified by anticancer heterocyclic complexes of ruthenium(III) in cell-free media
JF  - Journal of Biological Inorganic Chemistry
VL  - 6
IS  - 1
SP  - 435
EP  - 435
PB  - Springer-Verlag
SN  - 0949368
KW  - DNA
KW  - ruthenium
KW  - cytostatika
KW  - interakce
N2  - Modifications of natural DNA by three anticancer heterocyclic ruthenium(III) compounds were studied by methods of molecular biophysics. These methods include: DNA binding studies using atomic absorption spectrophotometry, inhibition of restriction endonucleases, mapping of DNA adducts by transcription assay, interstrand cross-linking employing gel electrophoresis under denaturing conditions, DNA unwinding studied by gel electrophoresis, circular dichroism analysis of B to Z transition in DNA and DNA melting curves measured by absorption spectrophotometry. It has been suggested that the altered DNA binding mode of ruthenium compounds in comparison with cisplatin might be an important factor responsible for altered cytostatic activity of this class of ruthenium compounds in tumor cells.
ER  -

MALINA, Jaroslav, Olga NOVÁKOVÁ, Bernhard K. KEPPLER, Enzo ALESSIO a Viktor BRABEC. Biophysical analysis of natural, double-helical DNA modified by anticancer heterocyclic complexes of ruthenium(III) in cell-free media. \textit{Journal of Biological Inorganic Chemistry}. germany: Springer-Verlag, 2001, roč.~6, č.~1, s.~435-445. ISSN~0949-368.

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