2001
Determination of phenolic compounds in artichoke extract by means of MEKC
ŠEVČÍKOVÁ, Petra, Zdeněk GLATZ, Jiří SLANINA a Pavel MUSILZákladní údaje
Originální název
Determination of phenolic compounds in artichoke extract by means of MEKC
Autoři
ŠEVČÍKOVÁ, Petra, Zdeněk GLATZ, Jiří SLANINA a Pavel MUSIL
Vydání
1. vyd. Verona, Book of abstract ICES 2001 An Electrophoretic Odessey, s. 80-80, 2001
Nakladatel
Emmezeta Congressi
Další údaje
Jazyk
angličtina
Typ výsledku
Stať ve sborníku
Obor
10600 1.6 Biological sciences
Stát vydavatele
Itálie
Utajení
není předmětem státního či obchodního tajemství
Kód RIV
RIV/00216224:14310/01:00004276
Organizační jednotka
Přírodovědecká fakulta
Klíčová slova anglicky
MEKC Schizandra Lignans
Štítky
Změněno: 3. 7. 2001 11:51, prof. RNDr. Zdeněk Glatz, CSc.
Anotace
V originále
Artichoke (Cynara) is well known as a vegetable, but it has also application in pharmacology. The choleretic and hypocholesterolemic actions of the artichoke extract are already known. The present research is being carried out into the antioxidative and hepatoprotective effects of such extracts. These activities have been attributed to the phenolic compounds presented in the extract and to their caffeoylquinic derivatives. MEKC has been developed as a promising method for the analysis of the Artichoke extract. Separation conditions have been optimised with respect to different parameters including pH and ionic strength of the background electrolyte, SDS concentration and temperature of capillary. A buffer consisting of 100 mM SDS in 20 mM sodium dihydrogen phosphate, 20 mM disodium tetraborate pH 8.5 was found to be the most suitable electrolyte for this separation. An applied voltage of 28 kV (positive polarity) and a temperature of 25 oC gave the best separation of the caffeoylquinic acid derivatives.
Návaznosti
| GA303/00/D062, projekt VaV |
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