Determination of Metallothionein at Femtomole Level by Constant
Current Stripping Chronopotentiometry

J 2001

Determination of Metallothionein at Femtomole Level by Constant Current Stripping Chronopotentiometry

KIZEK, René, Libuše TRNKOVÁ a Emil PALEČEK

Základní údaje

Originální název

Determination of Metallothionein at Femtomole Level by Constant Current Stripping Chronopotentiometry

Autoři

KIZEK, René, Libuše TRNKOVÁ a Emil PALEČEK

Vydání

Anal. Chem. USA, American Chemical Society, 2001, 0003-2700

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10405 Electrochemistry

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 4.532

Kód RIV

RIV/00216224:14310/01:00004521

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Metallothionein; Constant current stripping chronopotentiometry; Catalytic hydrogen evolution; Cyprinus carpio

Štítky

catalytic hydrogen evolution, Constant current stripping chronopotentiometry, Cyprinus carpio, metallothionein

Změněno: 8. 11. 2001 15:15, prof. RNDr. Libuše Trnková, CSc.

Anotace

V originále

Metallothionein (MT) from rabbit liver was analysed by differential pulse polarography (DPP with DME), cyclic voltammetry (CV), square wave voltammetry (SWV), and chronopotentiometric stripping analysis (CPSA) with hanging mercury drop electrode (HMDE) under various conditions. The highest sensitivity of the MT determination was obtained with CPSA which produced a well developed peak H due to catalytic hydrogen evolution at highly negative potentials. The highest peak H was obtained in borate buffer at pH 7.6. In this medium subnanomolar concentrations of MT were detectable. In the adsorptive transfer stripping (medium exchange) experiments determination of few femtomoles of MT in 5 ml aliquots of the analyte was possible. CPSA determination of MT in various tissues of carp (Cyprinus cyprio) yielded values in agreement with the published data.

Návaznosti

GV204/97/K084, projekt VaV

Název: Elektrody modifikované nukleovými kyselinami a bílkovinami. Nové nástroje v biochemickém a biomedicínském výzkumu

Investor: Grantová agentura ČR, Elektrody modifikované nukleovými kyselinami a bílkovinami. Nové nástroje v biochemickém a biomedicínském výzkumu

MSM 143100011, záměr

Název: Struktura a vazebné poměry, vlastnosti a analýza syntetických a přírodních molekulových ansamblů

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Struktura a vazebné poměry, vlastnosti a analýza syntetických a přírodních molekulových ansamblů

Citovat

KIZEK, René, Libuše TRNKOVÁ a Emil PALEČEK. Determination of Metallothionein at Femtomole Level by Constant Current Stripping Chronopotentiometry. Anal. Chem. USA: American Chemical Society, 2001, roč. 2001, č. 20, s. 4801-4808. ISSN 0003-2700.

@article{371378,
   author = {Kizek, René and Trnková, Libuše and Paleček, Emil},
   article_location = {USA},
   article_number = {20},
   keywords = {Metallothionein; Constant current stripping chronopotentiometry; Catalytic hydrogen evolution; Cyprinus carpio},
   language = {eng},
   issn = {0003-2700},
   journal = {Anal. Chem.},
   note = {Metallothionein, Constant current stripping chronopotentiometry, Catalytic hydrogen evolution, Cyprinus carpio},
   title = {Determination of Metallothionein at Femtomole Level by Constant Current Stripping Chronopotentiometry},
   volume = {2001},
   year = {2001}
}

TY  - JOUR
ID  - 371378
AU  - Kizek, René - Trnková, Libuše - Paleček, Emil
PY  - 2001
TI  - Determination of Metallothionein at Femtomole Level by Constant Current Stripping Chronopotentiometry
JF  - Anal. Chem.
VL  - 2001
IS  - 20
SP  - 4801
EP  - 4801
PB  - American Chemical Society
SN  - 00032700
N1  - Metallothionein, Constant current stripping chronopotentiometry, Catalytic hydrogen evolution, Cyprinus carpio
KW  - Metallothionein
KW  - Constant current stripping chronopotentiometry
KW  - Catalytic hydrogen evolution
KW  - Cyprinus carpio
N2  - Metallothionein (MT) from rabbit liver was analysed by differential pulse polarography (DPP with DME), cyclic voltammetry (CV), square wave voltammetry (SWV), and chronopotentiometric stripping analysis (CPSA) with hanging mercury drop electrode (HMDE) under various conditions. The highest sensitivity of the MT determination was obtained with CPSA which produced a well developed peak H due to catalytic hydrogen evolution at highly negative potentials. The highest peak H was obtained in borate buffer at pH 7.6. In this medium subnanomolar concentrations of MT were detectable. In the adsorptive transfer stripping (medium exchange) experiments determination of few femtomoles of MT in 5 ml aliquots of the analyte was possible. CPSA determination of MT in various tissues of carp (Cyprinus cyprio) yielded values in agreement with the published data.
ER  -

KIZEK, René, Libuše TRNKOVÁ a Emil PALEČEK. Determination of Metallothionein at Femtomole Level by Constant Current Stripping Chronopotentiometry. \textit{Anal. Chem.}. USA: American Chemical Society, 2001, roč.~2001, č.~20, s.~4801-4808. ISSN~0003-2700.

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