Activation or detoxification of mutagenic and carcinogenic
compounds in transgenic Drosophila expressing human glutathione
S-transferase.

J 2000

Activation or detoxification of mutagenic and carcinogenic compounds in transgenic Drosophila expressing human glutathione S-transferase.

CHROUST, Karel, Trevor JOWETT, Mustafa FARID-WAJIDI, Juan HUANG, Martina RYŠKOVÁ et. al.

Basic information

Original name

Activation or detoxification of mutagenic and carcinogenic compounds in transgenic Drosophila expressing human glutathione S-transferase.

Authors

CHROUST, Karel, Trevor JOWETT, Mustafa FARID-WAJIDI, Juan HUANG, Martina RYŠKOVÁ, Ronald WOLF and Ivan HOLOUBEK

Edition

Mutation Research, Amsterdam, Elsevier, 2000, 0165-1218

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/00:00004528

Organization unit

Faculty of Science

Keywords in English

Transgenic Drosophila melanogaster; wing spot test; female sterility; aneuploidy; glutathione S-transferase; 1;2:5;6-dibenzanthracene; 1;2-dichloroethane

Abstract

V originále

Sensitivity of transgenic Drosophila melanogaster with expression of a human gene encoding the glutathione S-transferase alpha subunit (GSTA1-1) to 1,2:5,6-dibenzanthracene (DBA) and 1,2-dichloroethane (DCE) was investigated in the somatic mutation and recombination test (SMART). We performed the same assay in control transgenic flies expressing the bacterial lacZ gene. Three types of transgenic Drosophila strains carrying GSTA1-1 were used: two transgenic strains homozygous for the second chromosome with a single-copy transgene insertion and one strain with two transgene insertions. Larvae carrying the lacZ gene were significantly more sensitive to genotoxic effects of DBA than those carrying three copies of the GSTA1-1 gene. The larvae with lacZ expression showed significantly lower sensitivity to DCE compared with those expressing GSTA1-1. Finally, a pretreatment with buthionine-sulphoximine (BSO) in experiment with DCE significantly decreased the frequency of mutation events in larvae with three GSTA1-1 copies in comparison with others.

Links

MSM 141100003, plan (intention)

Name: Zevní prostředí - karcinogeneze - onkologie

Investor: Ministry of Education, Youth and Sports of the CR, Environment - carcinogenesis - oncology

MSM 143100008, plan (intention)

Name: Genomy a jejich funkce

Investor: Ministry of Education, Youth and Sports of the CR, Genomes and their functions

Citovat

CHROUST, Karel, Trevor JOWETT, Mustafa FARID-WAJIDI, Juan HUANG, Martina RYŠKOVÁ, Ronald WOLF and Ivan HOLOUBEK. Activation or detoxification of mutagenic and carcinogenic compounds in transgenic Drosophila expressing human glutathione S-transferase. Mutation Research. Amsterdam: Elsevier, 2000, vol. 2001, 498(1-2), p. 169-179. ISSN 0165-1218.

@article{371654,
   author = {Chroust, Karel and Jowett, Trevor and FaridandWajidi, Mustafa and Huang, Juan and Ryšková, Martina and Wolf, Ronald and Holoubek, Ivan},
   article_location = {Amsterdam},
   article_number = {498(1-2)},
   keywords = {Transgenic Drosophila melanogaster; wing spot test; female sterility; aneuploidy; glutathione S-transferase; 1;2:5;6-dibenzanthracene; 1;2-dichloroethane},
   language = {eng},
   issn = {0165-1218},
   journal = {Mutation Research},
   title = {Activation or detoxification of mutagenic and carcinogenic compounds in transgenic Drosophila expressing human glutathione S-transferase.},
   volume = {2001},
   year = {2000}
}

TY  - JOUR
ID  - 371654
AU  - Chroust, Karel - Jowett, Trevor - Farid-Wajidi, Mustafa - Huang, Juan - Ryšková, Martina - Wolf, Ronald - Holoubek, Ivan
PY  - 2000
TI  - Activation or detoxification of mutagenic and carcinogenic compounds in transgenic Drosophila expressing human glutathione S-transferase.
JF  - Mutation Research
VL  - 2001
IS  - 498(1-2)
SP  - 169
EP  - 169
PB  - Elsevier
SN  - 01651218
KW  - Transgenic Drosophila melanogaster
KW  - wing spot test
KW  - female sterility
KW  - aneuploidy
KW  - glutathione S-transferase
KW  - 1;2:5;6-dibenzanthracene
KW  - 1;2-dichloroethane
N2  - Sensitivity of transgenic Drosophila melanogaster with expression of a human gene encoding the glutathione S-transferase alpha subunit (GSTA1-1) to 1,2:5,6-dibenzanthracene (DBA) and 1,2-dichloroethane (DCE) was investigated in the somatic mutation and recombination test (SMART). We performed the same assay in control transgenic flies expressing the bacterial lacZ gene. Three types of transgenic Drosophila strains carrying GSTA1-1 were used: two transgenic strains homozygous for the second chromosome with a single-copy transgene insertion and one strain with two transgene insertions. Larvae carrying the lacZ gene were significantly more sensitive to genotoxic effects of DBA than those carrying three copies of the GSTA1-1 gene. The larvae with lacZ expression showed significantly lower sensitivity to DCE compared with those expressing GSTA1-1. Finally, a pretreatment with buthionine-sulphoximine (BSO) in experiment with DCE significantly decreased the frequency of mutation events in larvae with three GSTA1-1 copies in comparison with others.
ER  -

CHROUST, Karel, Trevor JOWETT, Mustafa FARID-WAJIDI, Juan HUANG, Martina RYŠKOVÁ, Ronald WOLF and Ivan HOLOUBEK. Activation or detoxification of mutagenic and carcinogenic compounds in transgenic Drosophila expressing human glutathione S-transferase. \textit{Mutation Research}. Amsterdam: Elsevier, 2000, vol.~2001, 498(1-2), p.~169-179. ISSN~0165-1218.

Detailed Information on Publication Record