Developmental Consequences of ipt Activation in Germinating
Tobacco Seedlings

D 2001

Developmental Consequences of ipt Activation in Germinating Tobacco Seedlings

BRZOBOHATÝ, Břetislav; Todor Nikolaev GENKOV; Ian MOORE; Jaroslava DUBOVÁ; Eliška NEJEDLÁ et al.

Základní údaje

Originální název

Developmental Consequences of ipt Activation in Germinating Tobacco Seedlings

Autoři

BRZOBOHATÝ, Břetislav; Todor Nikolaev GENKOV; Ian MOORE; Jaroslava DUBOVÁ; Eliška NEJEDLÁ; Jiří MALBECK a Martin VÁGNER

Vydání

Brno, 17th International Conference on Plant Growth Substances, Brno, Czech Republic, July 1-6, 2001, Abstracts, s. 57-57, 2001

Nakladatel

Mendel University of Agriculture and Forestry, Brno

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

Genetika a molekulární biologie

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/01:00004563

Organizační jednotka

Přírodovědecká fakulta

ISBN

80-7157-518-6

Klíčová slova česky

transkripčně aktivační systém;izopentenyltransferáza; cytokininy; tabák; Arabidopsis

Klíčová slova anglicky

transcription activation system; isopentenyl transferase; cytokinins; tobacco; Arabidopsis

Změněno: 26. 3. 2010 13:10, RNDr. Eliška Nejedlá, CSc.

Anotace

V originále

A transcription activation system based on a synthetic promoter pOp and a corresponding chimeric transcription activator LhG4 was employed to achieve regulatable expression of isopentenyl transferase, IPT, in transgenic tobacco. The system allowed us to analyze in detail developmental consequences of ipt activation in germination and early seedling development. Here we present analysis of (i) dynamics of changes in cytokinin metabolism upon ipt activation and (ii) subsequent chain of developmental alterations leading to transition from originally almost normal seedling to a completely distinct teratoma structure. Major differences in tobacco and Arabidopsis response to ipt activation during germination will be discussed.

Návaznosti

IAA5004001, projekt VaV

Název: Systém aktivace transkripce pro studium vztahu mezi metabolizmem a účinkem cytokininů u Arabidopsis a tabáku

Investor: Akademie věd ČR, Systém aktivace transkripce pro studium vztahu mezi metabolizmem a účinkem cytokininů u Arabidopsis a tabáku

Citovat

BRZOBOHATÝ, Břetislav; Todor Nikolaev GENKOV; Ian MOORE; Jaroslava DUBOVÁ; Eliška NEJEDLÁ; Jiří MALBECK a Martin VÁGNER. Developmental Consequences of ipt Activation in Germinating Tobacco Seedlings. In 17th International Conference on Plant Growth Substances, Brno, Czech Republic, July 1-6, 2001, Abstracts. Brno: Mendel University of Agriculture and Forestry, Brno, 2001, s. 57. ISBN 80-7157-518-6.

@inproceedings{372651,
   author = {Brzobohatý, Břetislav and Genkov, Todor Nikolaev and Moore, Ian and Dubová, Jaroslava and Nejedlá, Eliška and Malbeck, Jiří and Vágner, Martin},
   address = {Brno},
   booktitle = {17th International Conference on Plant Growth Substances, Brno, Czech Republic, July 1-6, 2001, Abstracts},
   keywords = {transcription activation system; isopentenyl transferase; cytokinins; tobacco; Arabidopsis},
   language = {eng},
   location = {Brno},
   isbn = {80-7157-518-6},
   pages = {57-57},
   publisher = {Mendel University of Agriculture and Forestry, Brno},
   title = {Developmental Consequences of ipt Activation in Germinating Tobacco Seedlings},
   year = {2001}
}

TY  - CONF
ID  - 372651
AU  - Brzobohatý, Břetislav - Genkov, Todor Nikolaev - Moore, Ian - Dubová, Jaroslava - Nejedlá, Eliška - Malbeck, Jiří - Vágner, Martin
PY  - 2001
TI  - Developmental Consequences of ipt Activation in Germinating Tobacco Seedlings
PB  - Mendel University of Agriculture and Forestry, Brno
CY  - Brno
SN  - 8071575186
KW  - transcription activation system
KW  - isopentenyl transferase
KW  - cytokinins
KW  - tobacco
KW  - Arabidopsis
N2  - A transcription activation system based on a synthetic promoter pOp and a corresponding chimeric transcription activator LhG4 was employed to achieve regulatable expression of isopentenyl transferase, IPT, in transgenic tobacco. The system allowed us to analyze in detail developmental consequences of ipt activation in germination and early seedling development. Here we present analysis of (i) dynamics of changes in cytokinin metabolism upon ipt activation and (ii) subsequent chain of developmental alterations leading to transition from originally almost normal seedling to a completely distinct teratoma structure. Major differences in tobacco and Arabidopsis response to ipt activation during germination will be discussed.
ER  -

BRZOBOHATÝ, Břetislav; Todor Nikolaev GENKOV; Ian MOORE; Jaroslava DUBOVÁ; Eliška NEJEDLÁ; Jiří MALBECK a Martin VÁGNER. Developmental Consequences of ipt Activation in Germinating Tobacco Seedlings. In \textit{17th International Conference on Plant Growth Substances, Brno, Czech Republic, July 1-6, 2001, Abstracts}. Brno: Mendel University of Agriculture and Forestry, Brno, 2001, s.~57. ISBN~80-7157-518-6.

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