| BORKOVCOVÁ, Petra, Jan ZOUHAR, Jan HEJÁTKO a Břetislav BRZOBOHATÝ. A Receiver Domain of CKI1 Sensor Kinase: Expression, Purification and Antibody Production. In 17th International Conference on Plant Growth Substances, Brno, Czech Republic, July 1-6, 2001, Abstracts. Brno: Mendel University of Agriculture and Forestry, Brno, 2001, s. 151. ISBN 80-7157-518-6. |
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@inproceedings{372696,
author = {Borkovcová, Petra and Zouhar, Jan and Hejátko, Jan and Brzobohatý, Břetislav},
address = {Brno},
booktitle = {17th International Conference on Plant Growth Substances, Brno, Czech Republic, July 1-6, 2001, Abstracts},
language = {eng},
location = {Brno},
isbn = {80-7157-518-6},
pages = {151-151},
publisher = {Mendel University of Agriculture and Forestry, Brno},
title = {A Receiver Domain of CKI1 Sensor Kinase: Expression, Purification and Antibody Production},
year = {2001}
}
TY - JOUR
ID - 372696
AU - Borkovcová, Petra - Zouhar, Jan - Hejátko, Jan - Brzobohatý, Břetislav
PY - 2001
TI - A Receiver Domain of CKI1 Sensor Kinase: Expression, Purification and Antibody Production
PB - Mendel University of Agriculture and Forestry, Brno
CY - Brno
SN - 8071575186
N2 - CKI1, a putative cytokinin receptor of A. thaliana, belongs to a family of sensor hybrid histidine kinases. Here we report a successful production and single-step purification of the CKI1 signal receiver domain. The open reading frame coding for the domain was cloned into an expression vector pET-28a+ and the resulting double His-tag fusion protein was produced in E. coli. Western blot analysis revealed that the fusion protein is present mainly in a soluble fraction of the cell lysate. Single-step purification of the fusion protein was achieved by immobilized metal affinity chromatography. We will report protocol yielding milligram quantities of the recombinant protein in purity higher than 95% from single run of a column. The fusion protein was used to raise polyclonal antibodies in rabbits. Preliminary characterization of the antibodies will be presented.
ER -
BORKOVCOVÁ, Petra, Jan ZOUHAR, Jan HEJÁTKO a Břetislav BRZOBOHATÝ. A Receiver Domain of CKI1 Sensor Kinase: Expression, Purification and Antibody Production. In \textit{17th International Conference on Plant Growth Substances, Brno, Czech Republic, July 1-6, 2001, Abstracts}. Brno: Mendel University of Agriculture and Forestry, Brno, 2001, s.~151. ISBN~80-7157-518-6.
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