Regulated Expression of Isopentenyl Transferase in Arabidopsis:
System Development.

D 2001

Regulated Expression of Isopentenyl Transferase in Arabidopsis: System Development.

JIAN CHUN, Guo a Břetislav BRZOBOHATÝ

Základní údaje

Originální název

Regulated Expression of Isopentenyl Transferase in Arabidopsis: System Development.

Autoři

JIAN CHUN, Guo a Břetislav BRZOBOHATÝ

Vydání

Brno, 17th International Conference on Plant Growth Substances, Brno, Czech Republic, July 1-6, 2001, Abstracts, s. 88-88, 2001

Nakladatel

Mendel University of Agriculture and Forestry, Brno

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

Genetika a molekulární biologie

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14330/01:00004568

Organizační jednotka

Fakulta informatiky

ISBN

80-7157-518-6

Změněno: 23. 5. 2002 15:10, RNDr. JUDr. Vladimír Šmíd, CSc.

Anotace

V originále

A novel system allowing a non-invasive manipulation of cytokinin levels is being developed in A. thaliana. Transcriptional fusions between isopentenyl transferase gene, ipt, and a reporter gene, GUS, were inserted behind a synthetic promoter (pOp). pOp is expected to be inactive when introduced into wild type plants but is activated when these plants are crossed with others that express a novel transcription activation protein, LhG4. Transgenic Arabidopsis plants carrying pOp-ipt-GUS and pOp-GUS-ipt were generated by Agrobacterium infiltration technique. The transformed plants developed without any detectable phenotype alteration indicating that pOp promoter is virtually silent in the transformants in the absence of LhG4. The first evidence for activation of ipt and GUS in pOp-ipt-GUS and pOp-GUS-ipt was provided by crossing the individual transformants with an activator line carrying LhG4 under control of CaMV35S promoter (CaMV35S-LhG4). We will report phenotype alterations observed in progeny of crosses of pOp-ipt-GUS and pOp-GUS-ipt with CaMV35S-LhG4.

Návaznosti

IAA5004001, projekt VaV

Název: Systém aktivace transkripce pro studium vztahu mezi metabolizmem a účinkem cytokininů u Arabidopsis a tabáku

Investor: Akademie věd ČR, Systém aktivace transkripce pro studium vztahu mezi metabolizmem a účinkem cytokininů u Arabidopsis a tabáku

Citovat

JIAN CHUN, Guo a Břetislav BRZOBOHATÝ. Regulated Expression of Isopentenyl Transferase in Arabidopsis: System Development. In 17th International Conference on Plant Growth Substances, Brno, Czech Republic, July 1-6, 2001, Abstracts. Brno: Mendel University of Agriculture and Forestry, Brno, 2001, s. 88. ISBN 80-7157-518-6.

@inproceedings{372751,
   author = {Jian Chun, Guo and Brzobohatý, Břetislav},
   address = {Brno},
   booktitle = {17th International Conference on Plant Growth Substances, Brno, Czech Republic, July 1-6, 2001, Abstracts},
   language = {eng},
   location = {Brno},
   isbn = {80-7157-518-6},
   pages = {88-88},
   publisher = {Mendel University of Agriculture and Forestry, Brno},
   title = {Regulated Expression of Isopentenyl Transferase in Arabidopsis: System Development.},
   year = {2001}
}

TY  - CONF
ID  - 372751
AU  - Jian Chun, Guo - Brzobohatý, Břetislav
PY  - 2001
TI  - Regulated Expression of Isopentenyl Transferase in Arabidopsis: System Development.
PB  - Mendel University of Agriculture and Forestry, Brno
CY  - Brno
SN  - 8071575186
N2  - A novel system allowing a non-invasive manipulation of cytokinin levels is being developed in A. thaliana. Transcriptional fusions between isopentenyl transferase gene, ipt, and a reporter gene, GUS, were inserted behind a synthetic promoter (pOp). pOp is expected to be inactive when introduced into wild type plants but is activated when these plants are crossed with others that express a novel transcription activation protein, LhG4. Transgenic Arabidopsis plants carrying pOp-ipt-GUS and pOp-GUS-ipt were generated by Agrobacterium infiltration technique. The transformed plants developed without any detectable phenotype alteration indicating that pOp promoter is virtually silent in the transformants in the absence of LhG4. The first evidence for activation of ipt and GUS in pOp-ipt-GUS and pOp-GUS-ipt was provided by crossing the individual transformants with an activator line carrying LhG4 under control of CaMV35S promoter (CaMV35S-LhG4). We will report phenotype alterations observed in progeny of crosses of pOp-ipt-GUS and pOp-GUS-ipt with CaMV35S-LhG4.
ER  -

JIAN CHUN, Guo a Břetislav BRZOBOHATÝ. Regulated Expression of Isopentenyl Transferase in Arabidopsis: System Development. In \textit{17th International Conference on Plant Growth Substances, Brno, Czech Republic, July 1-6, 2001, Abstracts}. Brno: Mendel University of Agriculture and Forestry, Brno, 2001, s.~88. ISBN~80-7157-518-6.

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