Nitrate Reductase Activity in ipt-Expressing Tobacco.

D 2001

Nitrate Reductase Activity in ipt-Expressing Tobacco.

LEXA, Matej; Todor Nikolaev GENKOV a Břetislav BRZOBOHATÝ

Základní údaje

Originální název

Nitrate Reductase Activity in ipt-Expressing Tobacco.

Autoři

LEXA, Matej; Todor Nikolaev GENKOV a Břetislav BRZOBOHATÝ

Vydání

Brno, 17th International Conference on Plant Growth Substances, Brno, Czech Republic, July 1-6, 2001, Abstracts, s. 189-189, 2001

Nakladatel

Mendel University of Agriculture and Forestry, Brno

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

Genetika a molekulární biologie

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14330/01:00004570

Organizační jednotka

Fakulta informatiky

ISBN

80-7157-518-6

Změněno: 23. 5. 2002 13:19, RNDr. JUDr. Vladimír Šmíd, CSc.

Anotace

V originále

Using a novel system for expressing ipt gene in tobacco, we were able to increase cytokinins in plant tissue. This enabled us to study new regulatory aspects of nitrate (NO3-) reduction. We have grown plants with elevated cytokinins on MS with variable NO3- and benzyladenine (BA). We determined nitrate reductase activity (NRA) in this plant material. Initially, we found that ipt-expressing plants always display lower NRA than wild-type SR1 plants. We also found that long-term exposure of tobacco to cytokinins caused up to 60% decrease in NRA. However, addition of BA to N-starved plants caused induction of NRA to levels up to 100% higher than those before induction. In ipt-expressing plants this effect was only present if BA was administered together with NO3-. Exposure to 40mM NO3- caused up to 550% induction of NRA. This effect was always more pronounced in wild-type plants. We propose that regulation of NR by NO3- and cytokinins is a result of overlaying regulatory processes, with short-term ones having positive effect on NRA and those acting over extended periods having inhibitory effects on NRA.

Návaznosti

MSM 143100008, záměr

Název: Genomy a jejich funkce

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Genomy a jejich funkce

Citovat

LEXA, Matej; Todor Nikolaev GENKOV a Břetislav BRZOBOHATÝ. Nitrate Reductase Activity in ipt-Expressing Tobacco. In 17th International Conference on Plant Growth Substances, Brno, Czech Republic, July 1-6, 2001, Abstracts. Brno: Mendel University of Agriculture and Forestry, Brno, 2001, s. 189. ISBN 80-7157-518-6.

@inproceedings{372753,
   author = {Lexa, Matej and Genkov, Todor Nikolaev and Brzobohatý, Břetislav},
   address = {Brno},
   booktitle = {17th International Conference on Plant Growth Substances, Brno, Czech Republic, July 1-6, 2001, Abstracts},
   language = {eng},
   location = {Brno},
   isbn = {80-7157-518-6},
   pages = {189-189},
   publisher = {Mendel University of Agriculture and Forestry, Brno},
   title = {Nitrate Reductase Activity in ipt-Expressing Tobacco.},
   year = {2001}
}

TY  - CONF
ID  - 372753
AU  - Lexa, Matej - Genkov, Todor Nikolaev - Brzobohatý, Břetislav
PY  - 2001
TI  - Nitrate Reductase Activity in ipt-Expressing Tobacco.
PB  - Mendel University of Agriculture and Forestry, Brno
CY  - Brno
SN  - 8071575186
N2  - Using a novel system for expressing ipt gene in tobacco, we were able to increase cytokinins in plant tissue. This enabled us to study new regulatory aspects of nitrate (NO3-) reduction. We have grown plants with elevated cytokinins on MS with variable NO3- and benzyladenine (BA). We determined nitrate reductase activity (NRA) in this plant material. Initially, we found that ipt-expressing plants always display lower NRA than wild-type SR1 plants. We also found that long-term exposure of tobacco to cytokinins caused up to 60% decrease in NRA. However, addition of BA to N-starved plants caused induction of NRA to levels up to 100% higher than those before induction. In ipt-expressing plants this effect was only present if BA was administered together with NO3-. Exposure to 40mM NO3- caused up to 550% induction of NRA. This effect was always more pronounced in wild-type plants. We propose that regulation of NR by NO3- and cytokinins is a result of overlaying regulatory processes, with short-term ones having positive effect on NRA and those acting over extended periods having inhibitory effects on NRA.
ER  -

LEXA, Matej; Todor Nikolaev GENKOV a Břetislav BRZOBOHATÝ. Nitrate Reductase Activity in ipt-Expressing Tobacco. In \textit{17th International Conference on Plant Growth Substances, Brno, Czech Republic, July 1-6, 2001, Abstracts}. Brno: Mendel University of Agriculture and Forestry, Brno, 2001, s.~189. ISBN~80-7157-518-6.

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