Novel approach to monitoring of soil biological quality

D 2001

Novel approach to monitoring of soil biological quality

HOFMAN, Jakub a Jitka BEZCHLEBOVÁ

Základní údaje

Originální název

Novel approach to monitoring of soil biological quality

Autoři

HOFMAN, Jakub a Jitka BEZCHLEBOVÁ

Vydání

Krakow, Congress proceedings of SECOTOX 2001: Sixth European Conference on Ecotoxicology and Environmental Safety, Krakow - Poland, 20-24 August 2001, s. 206-531, 2001

Nakladatel

Art-Tekst

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/01:00004623

Organizační jednotka

Přírodovědecká fakulta

ISBN

83-88316-10-9

Změněno: 12. 11. 2001 15:30, prof. RNDr. Jakub Hofman, Ph.D.

Anotace

V originále

A classical chloroform fumigation-extraction method (CFEM) was carried out to obtain 0.5M K2SO4 extracts of fumigated (F) and non-fumigated (NF) samples of 36 grassland and 12 forest soils from Czech Republic. Main physical and chemical properties of soils were also determined and basal respiration (BR) of soils was measured. Organic C in extracts was determined by dichromate digestion. Anthrone-, orcinol-, and phenol-reactive C (ARC, ORC, and PRC, respectively), ninhydrin-reactive N (NRN), Folin-Ciocalteu's reagent-reactive compounds (FRC), and diphenylamine-reactive compounds (DRC) were assessed in extracts using colorimetric analytical methods. The methods used were found to be quick, simple and cheap. By this way biochemical specification of soil organic matter and microbial biomass was gained. Results of measurements were proposed to make several types of indices. The index expressions of measured parameters were found to be the most suitable for (a) evaluation and investigation of relationships between microbial biomass and soil organic matter, and for (b) soil organic matter composition studies. Three main types of indices were proposed: index I. as extracellular amount of each compound to microbial biomass, index II., "immobilization ratio", as proportion of each compound between soil extracellular organic matter and microbial biomass, and index III., "substrate quality index", as extracellular amount of each compound to extractable extracellular carbon (EX-C). The possibility of characterizing soil organic matter and microbial biomass C and N compounds by adopting suitable colorimetric methods is another practical advantage of CFEM and could show a possible course of improvement in soil microbial ecotoxicology.

Návaznosti

SI/340/1/00, projekt VaV

Název: Region specifický přístup v analýze ekologických rizik - metodika zhodnocení ve vztahu k přírodním katastrofám.

Citovat

HOFMAN, Jakub a Jitka BEZCHLEBOVÁ. Novel approach to monitoring of soil biological quality. In Congress proceedings of SECOTOX 2001: Sixth European Conference on Ecotoxicology and Environmental Safety, Krakow - Poland, 20-24 August 2001. Krakow: Art-Tekst, 2001, s. 206-531. ISBN 83-88316-10-9.

@inproceedings{374892,
   author = {Hofman, Jakub and Bezchlebová, Jitka},
   address = {Krakow},
   booktitle = {Congress proceedings of SECOTOX 2001: Sixth European Conference on Ecotoxicology and Environmental Safety, Krakow - Poland, 20-24 August 2001},
   language = {eng},
   location = {Krakow},
   isbn = {83-88316-10-9},
   pages = {206-531},
   publisher = {Art-Tekst},
   title = {Novel approach to monitoring of soil biological quality},
   year = {2001}
}

TY  - CONF
ID  - 374892
AU  - Hofman, Jakub - Bezchlebová, Jitka
PY  - 2001
TI  - Novel approach to monitoring of soil biological quality
PB  - Art-Tekst
CY  - Krakow
SN  - 8388316109
N2  - A classical chloroform fumigation-extraction method (CFEM) was carried out to obtain 0.5M K2SO4 extracts of fumigated (F) and non-fumigated (NF) samples of 36 grassland and 12 forest soils from Czech Republic. Main physical and chemical properties of soils were also determined and basal respiration (BR) of soils was measured. Organic C in extracts was determined by dichromate digestion. Anthrone-, orcinol-, and phenol-reactive C (ARC, ORC, and PRC, respectively), ninhydrin-reactive N (NRN), Folin-Ciocalteu's reagent-reactive compounds (FRC), and diphenylamine-reactive compounds (DRC) were assessed in extracts using colorimetric analytical methods. The methods used were found to be quick, simple and cheap. By this way biochemical specification of soil organic matter and microbial biomass was gained. Results of measurements were proposed to make several types of indices. The index expressions of measured parameters were found to be the most suitable for (a) evaluation and investigation of relationships between microbial biomass and soil organic matter, and for (b) soil organic matter composition studies. Three main types of indices were proposed: index I. as extracellular amount of each compound to microbial biomass, index II., "immobilization ratio", as proportion of each compound between soil extracellular organic matter and microbial biomass, and index III., "substrate quality index", as extracellular amount of each compound to extractable extracellular carbon (EX-C). The possibility of characterizing soil organic matter and microbial biomass C and N compounds by adopting suitable colorimetric methods is another practical advantage of CFEM and could show a possible course of improvement in soil microbial ecotoxicology.
ER  -

HOFMAN, Jakub a Jitka BEZCHLEBOVÁ. Novel approach to monitoring of soil biological quality. In \textit{Congress proceedings of SECOTOX 2001: Sixth European Conference on Ecotoxicology and Environmental Safety, Krakow - Poland, 20-24 August 2001}. Krakow: Art-Tekst, 2001, s.~206-531. ISBN~83-88316-10-9.

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