Properties of RNase A immobilized on magnetic
poly(2-hydroxyethyl methacrylate) microspheres

J 2001

Properties of RNase A immobilized on magnetic poly(2-hydroxyethyl methacrylate) microspheres

HORÁK, Daniel, Bohuslav RITTICH, Jan ŠAFÁŘ, Alena ŠPANOVÁ, Jiří LENFELD et. al.

Základní údaje

Originální název

Properties of RNase A immobilized on magnetic poly(2-hydroxyethyl methacrylate) microspheres

Autoři

HORÁK, Daniel, Bohuslav RITTICH (203 Česká republika, garant), Jan ŠAFÁŘ, Alena ŠPANOVÁ (203 Česká republika), Jiří LENFELD a Milan J. BENEŠ

Vydání

Biotechnology Progress, U.S.A. Am.Chem. Soc., Washington, 2001, 8756-7938

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Biotechnologie a bionika

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.821

Kód RIV

RIV/00216224:14310/01:00004811

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000169163400009

Klíčová slova anglicky

Dispersion polymerization; RNase A; nucleic acids; particles; polystyrene; chromatography; purification

Štítky

chromatography, Dispersion polymerization, nucleic acids, particles, polystyrene, purification, RNase A

Změněno: 13. 11. 2006 17:51, doc. Ing. Bohuslav Rittich, CSc.

Anotace

V originále

Magnetic hydrogel microsheres were prepared by dispersion copolymerisation of 2-hydroxyethyl methacrylate and ethylene dimethacrylate in the presence of magnetite, which formed the core of the particles. RNase A was coupled to the particles by the cyanuric chloride method. Gel electrophoresis of plasmid DNA pUC19 (contaminated by bacterial RNA) confirmed RNA degradation with the immobilized enzyme. The effect of temperature and pH on the relative activity of immobilized RNase A was estimated after incubation of the samples at different temperatures (30-80 C) and pH (4.0-8.0). Maximum relative activity was observed at 70 C and pH 6.5. The matrice based on magnetic poly(HEMA) had a low tendency to adsorb RNA.

Návaznosti

GA203/00/1339, projekt VaV

Název: Disperzní polymerizace hydrofilních monometrů pro přípravu magnetických nosičů používaných v biotechnologických aplikacích

Investor: Grantová agentura ČR, Disperzní polymerizace hydrofilních monometrů pro přípravu magnetických nosičů používaných v biotechnologických aplikacích

Citovat

HORÁK, Daniel, Bohuslav RITTICH, Jan ŠAFÁŘ, Alena ŠPANOVÁ, Jiří LENFELD a Milan J. BENEŠ. Properties of RNase A immobilized on magnetic poly(2-hydroxyethyl methacrylate) microspheres. Biotechnology Progress. U.S.A.: Am.Chem. Soc., Washington, 2001, roč. 17, č. 3, s. 447-452. ISSN 8756-7938.

@article{378510,
   author = {Horák, Daniel and Rittich, Bohuslav and Šafář, Jan and Španová, Alena and Lenfeld, Jiří and Beneš, Milan J.},
   article_location = {U.S.A.},
   article_number = {3},
   keywords = {Dispersion polymerization; RNase A; nucleic acids; particles; polystyrene; chromatography; purification},
   language = {eng},
   issn = {8756-7938},
   journal = {Biotechnology Progress},
   title = {Properties of RNase A immobilized on magnetic poly(2-hydroxyethyl methacrylate) microspheres},
   volume = {17},
   year = {2001}
}

TY  - JOUR
ID  - 378510
AU  - Horák, Daniel - Rittich, Bohuslav - Šafář, Jan - Španová, Alena - Lenfeld, Jiří - Beneš, Milan J.
PY  - 2001
TI  - Properties of RNase A immobilized on magnetic poly(2-hydroxyethyl methacrylate) microspheres
JF  - Biotechnology Progress
VL  - 17
IS  - 3
SP  - 447
EP  - 447
PB  - Am.Chem. Soc., Washington
SN  - 87567938
KW  - Dispersion polymerization
KW  - RNase A
KW  - nucleic acids
KW  - particles
KW  - polystyrene
KW  - chromatography
KW  - purification
N2  - Magnetic hydrogel microsheres were prepared by dispersion copolymerisation of 2-hydroxyethyl methacrylate and ethylene dimethacrylate in the presence of magnetite, which formed the core of the particles. RNase A was coupled to the particles by the cyanuric chloride method. Gel electrophoresis of plasmid DNA pUC19 (contaminated by bacterial RNA) confirmed RNA degradation with the immobilized enzyme. The effect of temperature and pH on the relative activity of immobilized RNase A was estimated after incubation of the samples at different temperatures (30-80 C) and pH (4.0-8.0). Maximum relative activity was observed at 70 C and pH 6.5. The matrice based on magnetic poly(HEMA) had a low tendency to adsorb RNA.
ER  -

HORÁK, Daniel, Bohuslav RITTICH, Jan ŠAFÁŘ, Alena ŠPANOVÁ, Jiří LENFELD a Milan J. BENEŠ. Properties of RNase A immobilized on magnetic poly(2-hydroxyethyl methacrylate) microspheres. \textit{Biotechnology Progress}. U.S.A.: Am.Chem. Soc., Washington, 2001, roč.~17, č.~3, s.~447-452. ISSN~8756-7938.

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