Application of HEMA-based particles in preparation of samples
for PCR identification of Salmonella cells in foods

D 2001

Application of HEMA-based particles in preparation of samples for PCR identification of Salmonella cells in foods

ŠPANOVÁ, Alena, Bohuslav RITTICH, Jiří LENFELD, Jana PRODĚLALOVÁ, Daniel HORÁK et. al.

Základní údaje

Originální název

Application of HEMA-based particles in preparation of samples for PCR identification of Salmonella cells in foods

Autoři

ŠPANOVÁ, Alena (203 Česká republika), Bohuslav RITTICH (203 Česká republika, garant), Jiří LENFELD, Jana PRODĚLALOVÁ (203 Česká republika), Daniel HORÁK a Milan J. BENEŠ

Vydání

2001. vyd. Zagreb, Croatia, The 4th Croatian Congress of Food Technologists, Biotechnologists and Nutritionists, s. 67-68, 2001

Nakladatel

Faculty of Food Technology and Biotechnology, University of Zagreb, Croatia

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Chorvatsko

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/01:00004819

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Salmonella cells; foods; PCR; preparation of samples; HEMA particles

Štítky

foods, HEMA particles, PCR, preparation of samples, Salmonella cells

Změněno: 13. 11. 2006 17:47, doc. Ing. Bohuslav Rittich, CSc.

Anotace

V originále

The quality of food products isalso affected by the microoragnisms contamination during the used technological processes.The aim of the research was to study the elimination of the inhibitor influence on the identification of target microorganisms in real samples using PCR. Salmonella, an important pathogen from hygienic point of wiev, was choosen as a model microorganism The polymerase chain reaction coupled with immunomagnetic separation (IMS-PCR) was used for the elimination of extracellular inhibitors and quick detection of Salmonella cells. The advantage of the use of proteinase K immobilized on poly(HEMA-co-EDMA) microspheres is discussed.

Návaznosti

GA203/00/1339, projekt VaV

Název: Disperzní polymerizace hydrofilních monometrů pro přípravu magnetických nosičů používaných v biotechnologických aplikacích

Investor: Grantová agentura ČR, Disperzní polymerizace hydrofilních monometrů pro přípravu magnetických nosičů používaných v biotechnologických aplikacích

Citovat

ŠPANOVÁ, Alena, Bohuslav RITTICH, Jiří LENFELD, Jana PRODĚLALOVÁ, Daniel HORÁK a Milan J. BENEŠ. Application of HEMA-based particles in preparation of samples for PCR identification of Salmonella cells in foods. In The 4th Croatian Congress of Food Technologists, Biotechnologists and Nutritionists. 2001. vyd. Zagreb, Croatia: Faculty of Food Technology and Biotechnology, University of Zagreb, Croatia, 2001, s. 67-68.

@inproceedings{378553,
   author = {Španová, Alena and Rittich, Bohuslav and Lenfeld, Jiří and Prodělalová, Jana and Horák, Daniel and Beneš, Milan J.},
   address = {Zagreb, Croatia},
   booktitle = {The 4th Croatian Congress of Food Technologists, Biotechnologists and Nutritionists},
   edition = {2001},
   keywords = {Salmonella cells; foods; PCR; preparation of samples; HEMA particles},
   language = {eng},
   location = {Zagreb, Croatia},
   pages = {67-68},
   publisher = {Faculty of Food Technology and Biotechnology, University of Zagreb, Croatia},
   title = {Application of HEMA-based particles in preparation of samples for PCR identification of Salmonella cells in foods},
   year = {2001}
}

TY  - JOUR
ID  - 378553
AU  - Španová, Alena - Rittich, Bohuslav - Lenfeld, Jiří - Prodělalová, Jana - Horák, Daniel - Beneš, Milan J.
PY  - 2001
TI  - Application of HEMA-based particles in preparation of samples for PCR identification of Salmonella cells in foods
PB  - Faculty of Food Technology and Biotechnology, University of Zagreb, Croatia
CY  - Zagreb, Croatia
KW  - Salmonella cells
KW  - foods
KW  - PCR
KW  - preparation of samples
KW  - HEMA particles
N2  - The quality of food products isalso affected by the microoragnisms contamination during the used technological processes.The aim of the research was to study the elimination of the inhibitor influence on the identification of target microorganisms in real samples using PCR. Salmonella, an important pathogen from hygienic point of wiev, was choosen as a model microorganism The polymerase chain reaction coupled with immunomagnetic separation (IMS-PCR) was used for the elimination of extracellular inhibitors and quick detection of Salmonella cells. The advantage of the use of proteinase K immobilized on poly(HEMA-co-EDMA) microspheres is discussed.
ER  -

ŠPANOVÁ, Alena, Bohuslav RITTICH, Jiří LENFELD, Jana PRODĚLALOVÁ, Daniel HORÁK a Milan J. BENEŠ. Application of HEMA-based particles in preparation of samples for PCR identification of Salmonella cells in foods. In \textit{The 4th Croatian Congress of Food Technologists, Biotechnologists and Nutritionists}. 2001. vyd. Zagreb, Croatia: Faculty of Food Technology and Biotechnology, University of Zagreb, Croatia, 2001, s.~67-68.

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