2001
Development of piezoelectric immunosensors for measurement of albuminuria
NAVRÁTILOVÁ, Iva; Petr SKLÁDAL a Vladimír VIKLICKÝZákladní údaje
Originální název
Development of piezoelectric immunosensors for measurement of albuminuria
Autoři
NAVRÁTILOVÁ, Iva; Petr SKLÁDAL a Vladimír VIKLICKÝ
Vydání
Talanta, Elsevier Science, 2001, 0039-9140
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
10600 1.6 Biological sciences
Stát vydavatele
Nizozemské království
Utajení
není předmětem státního či obchodního tajemství
Impakt faktor
Impact factor: 1.587
Kód RIV
RIV/00216224:14310/01:00004854
Organizační jednotka
Přírodovědecká fakulta
UT WoS
000173288900020
Klíčová slova anglicky
Immunosensor; Quartz crystal microbalance; Human serum albumin; Albuminuria
Změněno: 5. 12. 2001 10:35, prof. RNDr. Petr Skládal, CSc.
Anotace
V originále
The development of piezoelectric immunosensors for human serum albumin (HSA) is reported. The piezoelectric crystals were modified either with monoclonal antibody AL-01 (direct assay) or with HSA (competitive assay). Measurements were carried out in the flow-through mode. Affinity interaction between albumin and the antibody was characterised. With immobilised antibody and HSA in solution, the kinetic association rate constant ka was 18100 l mol-1 s-1 and the dissociation constant kd was 0.00369 s-1. For the opposite arrangement (immobilised HSA), a slower dissociation was observed, kd was 0.00085 s-1. A competitive assay for HSA was developed with working range of 1 to 5 ug/ml and a total time for one analysis equal to 17 min. Samples of urine were analysed after tenfold dilution. The developed system was successfully evaluated on real samples from diabetic patients and the obtained results correlated well with the standard reflectometric assay of proteins in urine.
Návaznosti
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