Immunohistochemical and immunogold analysis of laminins in the
peripheral nerve structures

J 2001

Immunohistochemical and immunogold analysis of laminins in the peripheral nerve structures

DUBOVÝ, Petr; Ivana SVÍŽENSKÁ; Ilona KLUSÁKOVÁ a Nabil Ahmed GHALIB

Základní údaje

Originální název

Immunohistochemical and immunogold analysis of laminins in the peripheral nerve structures

Autoři

DUBOVÝ, Petr; Ivana SVÍŽENSKÁ; Ilona KLUSÁKOVÁ a Nabil Ahmed GHALIB

Vydání

The Histochemical Journal, Holandsko, Kluwer Academic Publishers, 2001, 0018-2214

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30000 3. Medical and Health Sciences

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.169

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/01:00004875

Organizační jednotka

Lékařská fakulta

Změněno: 3. 10. 2007 09:24, prof. RNDr. Petr Dubový, CSc.

Anotace

V originále

The basal laminae of the peripheral nervous system are produced by Schwann and perineurial cells. Laminins, predominant glycoproteins of the basal laminae, play a role in adherence, growth, morphology, migration, and differentiation of the cells. There is conclusive evidence that laminin molecules promote the axon outgrowth and migration of Schwann cells. Laminins are heterotrimers composed of a,b and g chains that combine to form at least 11 isoforms. It is assumed that different compartments of the peripheral nervous system exhibit different laminin isoforms. Therefore, we have localised a distribution of laminin-1, -2, and -3 isoforms and some individual laminin chains (a1,b2,b3,g2) in the rat femoral nerve and Pacinian corpuscles by indirect immunofluorescence technique. In addition, location of laminin-1,-2, and -3 isoforms were studied in Pacinian corpuscles by immunogold electron microscopic technique. The results of immunohistochemical and immunogold detection of laminins confirmed that different compartments of the peripheral nervous system exhibit different laminin isoforms.

Návaznosti

GA309/00/0407, projekt VaV

Název: Celulární a extracelulární prostředí pro regeneraci axonů při kořenových a distálních lezích periferních nervů

Investor: Grantová agentura ČR, Celulární a extracelulární prostředí pro regeneraci axonů při kořenových a distálních lezích periferních nervů

Citovat

DUBOVÝ, Petr; Ivana SVÍŽENSKÁ; Ilona KLUSÁKOVÁ a Nabil Ahmed GHALIB. Immunohistochemical and immunogold analysis of laminins in the peripheral nerve structures. The Histochemical Journal. Holandsko: Kluwer Academic Publishers, 2001, roč. 33, č. 4, s. 233-234. ISSN 0018-2214.

@article{379343,
   author = {Dubový, Petr and Svíženská, Ivana and Klusáková, Ilona and Ghalib, Nabil Ahmed},
   article_location = {Holandsko},
   article_number = {4},
   language = {eng},
   issn = {0018-2214},
   journal = {The Histochemical Journal},
   title = {Immunohistochemical and immunogold analysis of laminins in the peripheral nerve structures},
   volume = {33},
   year = {2001}
}

TY  - JOUR
ID  - 379343
AU  - Dubový, Petr - Svíženská, Ivana - Klusáková, Ilona - Ghalib, Nabil Ahmed
PY  - 2001
TI  - Immunohistochemical and immunogold analysis of laminins in the peripheral nerve structures
JF  - The Histochemical Journal
VL  - 33
IS  - 4
SP  - 233
EP  - 233
PB  - Kluwer Academic Publishers
SN  - 00182214
N2  - The basal laminae of the peripheral nervous system are produced by Schwann and perineurial cells. Laminins, predominant glycoproteins of the basal laminae, play a role in adherence, growth, morphology, migration, and differentiation of the cells. There is conclusive evidence that laminin molecules promote the axon outgrowth and migration of Schwann cells. Laminins are heterotrimers composed of a,b and g chains that combine to form at least 11 isoforms. It is assumed that different compartments of the peripheral nervous system exhibit different laminin isoforms. Therefore, we have localised a distribution of laminin-1, -2, and -3 isoforms and some individual laminin chains (a1,b2,b3,g2) in the rat femoral nerve and Pacinian corpuscles by indirect immunofluorescence technique. In addition, location of laminin-1,-2, and -3 isoforms were studied in Pacinian corpuscles by immunogold electron microscopic technique. The results of immunohistochemical and immunogold detection of laminins confirmed that different compartments of the peripheral nervous system exhibit different laminin isoforms.
ER  -

DUBOVÝ, Petr; Ivana SVÍŽENSKÁ; Ilona KLUSÁKOVÁ a Nabil Ahmed GHALIB. Immunohistochemical and immunogold analysis of laminins in the peripheral nerve structures. \textit{The Histochemical Journal}. Holandsko: Kluwer Academic Publishers, 2001, roč.~33, č.~4, s.~233-234. ISSN~0018-2214.

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