| BOUCHAL, Pavel, Zdeněk GLATZ, Oldřich JANICZEK a Martin MANDL. Application of Capillary Zone Electrophoresis to Study the Properties of Rhodanese from Acidithiobacillus ferrooxidans. Folia Microbiologica. Praha: Institute of Microbiology of AV ČR, 2001, roč. 46, č. 5, s. 385-389. ISSN 0015-5632. |
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@article{383412,
author = {Bouchal, Pavel and Glatz, Zdeněk and Janiczek, Oldřich and Mandl, Martin},
article_location = {Praha},
article_number = {5},
keywords = {rhodanese; CZE},
language = {eng},
issn = {0015-5632},
journal = {Folia Microbiologica},
title = {Application of Capillary Zone Electrophoresis to Study the Properties of Rhodanese from Acidithiobacillus ferrooxidans},
volume = {46},
year = {2001}
}
TY - JOUR
ID - 383412
AU - Bouchal, Pavel - Glatz, Zdeněk - Janiczek, Oldřich - Mandl, Martin
PY - 2001
TI - Application of Capillary Zone Electrophoresis to Study the Properties of Rhodanese from Acidithiobacillus ferrooxidans
JF - Folia Microbiologica
VL - 46
IS - 5
SP - 385
EP - 385
PB - Institute of Microbiology of AV ČR
SN - 00155632
KW - rhodanese
KW - CZE
N2 - The new capillary zone electrophoretic method was applied for the assay of the enzymatic activity of the rhodanese from Acidithiobacillus ferrooxidans. The enzyme activities determined by capillary zone electrophoresis were compared with those determined by discontinuous spectrophotometric method. The values obtained were in a good agreement. The method was also used to evaluate Michaelis constants for both substrates; a new approach was developed to solve the problem with variable ionic strength of the samples. The other properties of the enzyme such as the pH and temperature optima were determined as well.
ER -
BOUCHAL, Pavel, Zdeněk GLATZ, Oldřich JANICZEK a Martin MANDL. Application of Capillary Zone Electrophoresis to Study the Properties of Rhodanese from Acidithiobacillus ferrooxidans. \textit{Folia Microbiologica}. Praha: Institute of Microbiology of AV ČR, 2001, roč.~46, č.~5, s.~385-389. ISSN~0015-5632.
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