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@article{384163,
author = {Svoboda, Augustin and Slaninová, Iva and Holubářová, Alena},
article_location = {Budapest},
article_number = {2},
keywords = {yeast; protoplasts; cell wall; actin; microtubules; cell polarity},
language = {eng},
issn = {0236-5383-01},
journal = {Acta Biologica Hungarica},
title = {Cytoskeleton in regenerating protoplasts and restoration of cell polarity in the yeast Saccharomyces cerevisiae},
volume = {52},
year = {2001}
}
TY - JOUR
ID - 384163
AU - Svoboda, Augustin - Slaninová, Iva - Holubářová, Alena
PY - 2001
TI - Cytoskeleton in regenerating protoplasts and restoration of cell polarity in the yeast Saccharomyces cerevisiae
JF - Acta Biologica Hungarica
VL - 52
IS - 2
SP - 325
EP - 325
PB - Akademiai Kiado
SN - 0236538301
KW - yeast
KW - protoplasts
KW - cell wall
KW - actin
KW - microtubules
KW - cell polarity
N2 - The actin cables and microtubules were disrupted during protoplasting in Saccharomyces cerevisiae cells. In the process of protoplast regeneration, the cytoplasmic microtubules were the first to be restored; actin patches remained regularly distributed under the surface of growing protoplast. After the cell wall had been regenerated in a gelatine medium, actin patches aggregated into clusters, which marked the site of bud development. An incomplete cell wall was the apparent cause for uncoupling between karyokinesis and cytokinesis The presence of several nuclei in the cell gave rise to several buds emerging simultaneously and was probably related to their random positions. In haploids, however, the axial type of budding was obvious also in the reverting protoplasts. Our observations suggest that actin is a structure involved in the restoration of polar growth during protoplast regeneration and that the cell wall plays an important role in this process: in its absence, actin fails to polarise.
ER -
SVOBODA, Augustin, Iva SLANINOVÁ a Alena HOLUBÁŘOVÁ. Cytoskeleton in regenerating protoplasts and restoration of cell polarity in the yeast Saccharomyces cerevisiae. \textit{Acta Biologica Hungarica}. Budapest: Akademiai Kiado, 2001, roč.~52, č.~2, s.~325-332. ISSN~0236-5383-01.
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