Genotype variability of TSST-1 positive Staphylococcus aureus
strains in the Czech Republic

D 2001

Genotype variability of TSST-1 positive Staphylococcus aureus strains in the Czech Republic

HRSTKA, Roman, Vladislava RŮŽIČKOVÁ, Roman PANTŮČEK, Petr PETRÁŠ, Jiří DOŠKAŘ et. al.

Základní údaje

Originální název

Genotype variability of TSST-1 positive Staphylococcus aureus strains in the Czech Republic

Autoři

HRSTKA, Roman (203 Česká republika), Vladislava RŮŽIČKOVÁ (203 Česká republika), Roman PANTŮČEK (203 Česká republika), Petr PETRÁŠ a Jiří DOŠKAŘ (203 Česká republika)

Vydání

Brno, V. Pracovní setkání biochemiků a molekulárních biologů, s. 25-25, 2001

Nakladatel

Masarykova univerzita v Brně

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

Genetika a molekulární biologie

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/01:00005395

Organizační jednotka

Přírodovědecká fakulta

ISBN

80-210-2538-7

Změněno: 30. 10. 2003 15:34, prof. RNDr. Roman Pantůček, Ph.D.

Anotace

V originále

Species Staphylococcus aureus belongs to clinically important gram-positive cocci. Some S. aureus strains produce TSST-1 toxin, which causes human disease called Toxic Shock Syndrom (TSS). Two forms of TSS were differentiated: the first type occurs in connection with menses, the second one is formed of cases, when TSS is a complication of another staphylococcal disease, for exampte food poisoning. Data about abiGty of S. aureus strains to produce TSST-1 toxin are clearly different. Therefore, we analyzed genomic DNAs of 26 S. aureus strains isolated from patients in the Czech Republic in connection with TSS. Staphylococcal isolates were analyzed by PCR using specific oligonucleotide primers to confirm presence of specific sequences of tst gene. To determine genetic variability, we performed macrorestriction analysis using PFGE, which enables determination of 20 different PFGE types. Strains were divided into 8 PFGE groups (ET1-ET8) according to Smal restriction patteros based on the similarity level between 65 - l00%. Majority of strains were classified into the group ET7, in whicb 14 strains were included (67% from all analyzed TSST-1 positive strains). The next method was PCR-amplification of variable-length 16S-23S rDNA spacer regions. The analyzed strains were distributed into 4 groups (SP1-SP4). Ztie distribution of strains into groups SP1-SP4 confirmed results gained by RFLP. In the next part of our work, we identified localization of tss specific sequences. The tst sequence was locatized on staphylococcal chromosome using hybridization by means of specific probe to SmaI DNA restriction patterns of S. aureus strains. Positive signals conesponded with ability of the strains to grow in Tip free medium, what indicates linkage of Trp operon and tst gene.

Návaznosti

MSM 143100008, záměr

Název: Genomy a jejich funkce

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Genomy a jejich funkce

Citovat

HRSTKA, Roman, Vladislava RŮŽIČKOVÁ, Roman PANTŮČEK, Petr PETRÁŠ a Jiří DOŠKAŘ. Genotype variability of TSST-1 positive Staphylococcus aureus strains in the Czech Republic. In V. Pracovní setkání biochemiků a molekulárních biologů. Brno: Masarykova univerzita v Brně, 2001, s. 25. ISBN 80-210-2538-7.

@inproceedings{390512,
   author = {Hrstka, Roman and Růžičková, Vladislava and Pantůček, Roman and Petráš, Petr and Doškař, Jiří},
   address = {Brno},
   booktitle = {V. Pracovní setkání biochemiků a molekulárních biologů},
   language = {eng},
   location = {Brno},
   isbn = {80-210-2538-7},
   pages = {25-25},
   publisher = {Masarykova univerzita v Brně},
   title = {Genotype variability of TSST-1 positive Staphylococcus aureus strains in the Czech Republic},
   year = {2001}
}

TY  - JOUR
ID  - 390512
AU  - Hrstka, Roman - Růžičková, Vladislava - Pantůček, Roman - Petráš, Petr - Doškař, Jiří
PY  - 2001
TI  - Genotype variability of TSST-1 positive Staphylococcus aureus strains in the Czech Republic
PB  - Masarykova univerzita v Brně
CY  - Brno
SN  - 8021025387
N2  - Species Staphylococcus aureus belongs to clinically important gram-positive cocci. Some S. aureus strains produce TSST-1 toxin, which causes human disease called Toxic Shock Syndrom (TSS). Two forms of TSS were differentiated: the first type occurs in connection with menses, the second one is formed of cases, when TSS is a complication of another staphylococcal disease, for exampte food poisoning. Data about abiGty of S. aureus strains to produce TSST-1 toxin are clearly different. Therefore, we analyzed genomic DNAs of 26 S. aureus strains isolated from patients in the Czech Republic in connection with TSS. Staphylococcal isolates were analyzed by PCR using specific oligonucleotide primers to confirm presence of specific sequences of tst gene. To determine genetic variability, we performed macrorestriction analysis using PFGE, which enables determination of 20 different PFGE types. Strains were divided into 8 PFGE groups (ET1-ET8) according to Smal restriction patteros based on the similarity level between 65 - l00%. Majority of strains were classified into the group ET7, in whicb 14 strains were included (67% from all analyzed TSST-1 positive strains). The next method was PCR-amplification of variable-length 16S-23S rDNA spacer regions. The analyzed strains were distributed into 4 groups (SP1-SP4). Ztie distribution of strains into groups SP1-SP4 confirmed results gained by RFLP. In the next part of our work, we identified localization of tss specific sequences. The tst sequence was locatized on staphylococcal chromosome using hybridization by means of specific probe to SmaI DNA restriction patterns of S. aureus strains. Positive signals conesponded with ability of the strains to grow in Tip free medium, what indicates linkage of Trp operon and tst gene.
ER  -

HRSTKA, Roman, Vladislava RŮŽIČKOVÁ, Roman PANTŮČEK, Petr PETRÁŠ a Jiří DOŠKAŘ. Genotype variability of TSST-1 positive $<$I$>$Staphylococcus aureus$<$/I$>$ strains in the Czech Republic. In \textit{V. Pracovní setkání biochemiků a molekulárních biologů}. Brno: Masarykova univerzita v Brně, 2001, s.~25. ISBN~80-210-2538-7.

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