Immunohistochemical analysis of some ECM components in the
nerve outgrowth regenerating through the knitted tubes

D 2001

Immunohistochemical analysis of some ECM components in the nerve outgrowth regenerating through the knitted tubes

SVÍŽENSKÁ, Ivana a Petr DUBOVÝ

Základní údaje

Originální název

Immunohistochemical analysis of some ECM components in the nerve outgrowth regenerating through the knitted tubes

Název česky

Imunohistochemická analysa složení ECM v nervovém regenerátu v pletené nervové protéze

Autoři

SVÍŽENSKÁ, Ivana a Petr DUBOVÝ

Vydání

Praha, Sborník lékařský, s. 284-284, 2001

Nakladatel

3.LF UK

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/01:00005398

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

extracellular matrix; peripheral nerve regeneration

Štítky

extracellular matrix, peripheral nerve regeneration

Změněno: 26. 6. 2009 10:59, prof. RNDr. Petr Dubový, CSc.

Anotace

V originále

The synthesis of the extracellular matrix (ECM) is inevitable condition for the peripheral nerve regeneration. In our experiments, we investigated several components of the ECM (fibronectin, collagen IV, thrombospondin, chondroitin sulfate, and vitronectin) by the indirect immunohistochemical method in cryostat cross sections through nerve regenerates obtained from 6 adult female rats. The regenerate rejoined the proximal and distal stumps of the transected sciatic nerve that had been inserted into the guide prepared from the knitted vascular prosthesis. After 6 weeks from the transection, the regenerate consisted of a band of axons arranged in fascicles running along the longitudinal axis of the prosthesis. The immunostaining for fibronectin, collagen IV, thrombospondin, and chondroitin sulfate was present in the sections. The immunoreaction was found in the connective tissue at the surface of the regenerate as well as in the perineurium of nerve fascicles. No immunoreactivity for vitronectin was observed in the whole regenerate. Our immunohistochemical results suggest that the ECM produced in the knitted vascular prosthesis provides a milieu suitable for nerve regeneration.

Návaznosti

GA309/00/0407, projekt VaV

Název: Celulární a extracelulární prostředí pro regeneraci axonů při kořenových a distálních lezích periferních nervů

Investor: Grantová agentura ČR, Celulární a extracelulární prostředí pro regeneraci axonů při kořenových a distálních lezích periferních nervů

Citovat

SVÍŽENSKÁ, Ivana a Petr DUBOVÝ. Immunohistochemical analysis of some ECM components in the nerve outgrowth regenerating through the knitted tubes. In Sborník lékařský. Praha: 3.LF UK, 2001, s. 284.

@inproceedings{390517,
   author = {Svíženská, Ivana and Dubový, Petr},
   address = {Praha},
   booktitle = {Sborník lékařský},
   keywords = {extracellular matrix; peripheral nerve regeneration},
   language = {eng},
   location = {Praha},
   pages = {284-284},
   publisher = {3.LF UK},
   title = {Immunohistochemical analysis of some ECM components in the nerve outgrowth regenerating through the knitted tubes},
   year = {2001}
}

TY  - CONF
ID  - 390517
AU  - Svíženská, Ivana - Dubový, Petr
PY  - 2001
TI  - Immunohistochemical analysis of some ECM components in the nerve outgrowth regenerating through the knitted tubes
PB  - 3.LF UK
CY  - Praha
KW  - extracellular matrix
KW  - peripheral nerve regeneration
N2  - The synthesis of the extracellular matrix (ECM) is inevitable condition for the peripheral nerve regeneration. In our experiments, we investigated several components of the ECM (fibronectin, collagen IV, thrombospondin, chondroitin sulfate, and vitronectin) by the indirect immunohistochemical method in cryostat cross sections through nerve regenerates obtained from 6 adult female rats. The regenerate rejoined the proximal and distal stumps of the transected sciatic nerve that had been inserted into the guide prepared from the knitted vascular prosthesis. After 6 weeks from the transection, the regenerate consisted of a band of axons arranged in fascicles running along the longitudinal axis of the prosthesis. The immunostaining for fibronectin, collagen IV, thrombospondin, and chondroitin sulfate was present in the sections. The immunoreaction was found in the connective tissue at the surface of the regenerate as well as in the perineurium of nerve fascicles. No immunoreactivity for vitronectin was observed in the whole regenerate. Our immunohistochemical results suggest that the ECM produced in the knitted vascular prosthesis provides a milieu suitable for nerve regeneration.
ER  -

SVÍŽENSKÁ, Ivana a Petr DUBOVÝ. Immunohistochemical analysis of some ECM components in the nerve outgrowth regenerating through the knitted tubes. In \textit{Sborník lékařský}. Praha: 3.LF UK, 2001, s.~284.

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