REJTAR, Tomas, Hu PING, Peter JUHASZ, Jennifer M. CAMPBELL, Marvin L. VESTAL, Jan PREISLER a Barry, L. KARGER. Off-Line Coupling of High-Resolution Capillary Electrophoresis to MALDI-TOF and TOF/TOF MS. Journal of Proteome Research. Washington, USA: ACS, 2002, roč. 1, č. 2, s. 171-179. ISSN 1535-3893.
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Základní údaje
Originální název Off-Line Coupling of High-Resolution Capillary Electrophoresis to MALDI-TOF and TOF/TOF MS
Autoři REJTAR, Tomas (203 Česká republika), Hu PING (156 Čína), Peter JUHASZ (840 Spojené státy), Jennifer M. CAMPBELL (840 Spojené státy), Marvin L. VESTAL (840 Spojené státy), Jan PREISLER (203 Česká republika, garant) a Barry, L. KARGER (840 Spojené státy).
Vydání Journal of Proteome Research, Washington, USA, ACS, 2002, 1535-3893.
Další údaje
Originální jazyk angličtina
Typ výsledku Článek v odborném periodiku
Obor 10406 Analytical chemistry
Stát vydavatele Spojené státy
Utajení není předmětem státního či obchodního tajemství
Kód RIV RIV/00216224:14310/02:00008546
Organizační jednotka Přírodovědecká fakulta
UT WoS 000178238600008
Klíčová slova anglicky MALDI; mass spectrometry; capillary electrophoresis; proteomics; proteins; peptides; interface;
Štítky Capillary electrophoresis, interface, MALDI, mass spectrometry, peptides, proteins, proteomics
Změnil Změnil: prof. Mgr. Jan Preisler, Ph.D., učo 45329. Změněno: 27. 6. 2009 23:58.
Anotace
High-resolution capillary electrophoresis has been coupled to MALDI-TOF and TOF/TOF MS through off-line vacuum deposition onto standard stainless steel MALDI targets. This off-line approach allowed the decoupling of the separation from the MS analysis, thus allowing each to be independently optimized in terms of time. Using BSA tryptic digest as a model sample, the deposited streaks, roughly 100-ím wide, were first analyzed in the MS mode, consuming only a fraction of the sample. After data analysis, segments of the deposited trace, containing unidentified peptides, as well as several species chosen for sequence confirmation, were reanalyzed in the MS/MS mode using MALDI-TOF/TOF MS. Additionally, it is shown that the shot-to-shot reproducibility of the vacuum-deposited trace (5% RSD) is 1 order of magnitude lower than that found for the standard dried droplet method. Moreover, a linear dependence of signal intensities (relative to an internal standard) over 3 orders of magnitude was found for a peptide sample with concentrations ranging from 1 to 1000 nM. This paper demonstrates the potential of off-line coupling of high-resolution separations to MALDI-MS and MALDI-MS/MS using vacuum deposition for the analysis of complex peptide mixtures from protein digests.
VytisknoutZobrazeno: 17. 6. 2024 04:11