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@article{397102,
author = {Gampfer, J. and Thon, Vojtěch and Gulle, H. and Wolf, Hermann and Eibl, Marta},
article_number = {9-10},
keywords = {TSST-1 vaccine},
language = {eng},
issn = {0264-410X},
journal = {Vaccine},
title = {Double mutant and formaldehyde inactivated TSST-1 as vaccine candidates},
volume = {20},
year = {2002}
}
TY - JOUR
ID - 397102
AU - Gampfer, J. - Thon, Vojtěch - Gulle, H. - Wolf, Hermann - Eibl, Marta
PY - 2002
TI - Double mutant and formaldehyde inactivated TSST-1 as vaccine candidates
JF - Vaccine
VL - 20
IS - 9-10
SP - 1354-64
EP - 1354-64
SN - 0264410X
KW - TSST-1 vaccine
N2 - Up to now there is no treatment for staphylococcal toxic shock syndrome, a disease mainly induced by toxic shock syndrome toxin-1(TSST-1). There is great demand in finding means to control the disease, one of them is the development of an effective and safe vaccine against TSST-1. In this study we constructed a series of vaccine candidates and investigated their biological activity, toxicity, and potential to invoke an immune response. TSST-1 was isolated from Stahylococcus aureus supernatants and recombinantly expressed as a N-terminal 6x histidine-tagged protein in Escherichia coli. In order to obtain molecules with minimal toxicity we constructed single mutants (G31R and H135A) and one double mutant (G31R/H135A) with both residues exchanged. We also detoxified native TSST-1 isolated from S. aureus, and recombinantly expressed TSST-1 by treatment with formaldehyde. Functional activity of native and recombinant TSST-1 and grade of inocuity of mutants and toxoids was determined by investigating mitogenity, T-cell activation, and cytokine release upon stimulation of human mononuclear cells with the vaccine candidates. All substances were tested in a rabbit immunization study. After primary immunization and three additional boosts all vaccinated animals developed antibody titers against TSST-1 and were protected against challenge with a lethal doses of superantigen potentiated with lipopolysaccharide.
ER -
GAMPFER, J., Vojtěch THON, H. GULLE, Hermann WOLF a Marta EIBL. Double mutant and formaldehyde inactivated TSST-1 as vaccine candidates. \textit{Vaccine}. 2002, roč.~20, 9-10, s.~1354-64, 11 s. ISSN~0264-410X.
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