LEXA, Matej, Todor Nikolaev GENKOV and Břetislav BRZOBOHATÝ. Inhibitory effects of elevated endogenous cytokinins on nitrate reductase in ipt-expressing tobacco are eliminated by short-term exposure to benzyladenine. PHYSIOLOGIA PLANTARUM, Copenhagen: Munksgaard, 2002, vol. 115, No 2, p. 284-290. ISSN 0031-9317.
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Basic information
Original name Inhibitory effects of elevated endogenous cytokinins on nitrate reductase in ipt-expressing tobacco are eliminated by short-term exposure to benzyladenine
Authors LEXA, Matej (703 Slovakia), Todor Nikolaev GENKOV (100 Bulgaria) and Břetislav BRZOBOHATÝ (203 Czechia, guarantor).
Edition PHYSIOLOGIA PLANTARUM, Copenhagen, Munksgaard, 2002, 0031-9317.
Other information
Original language English
Type of outcome article in a journal
Field of Study 10600 1.6 Biological sciences
Country of publisher Czechia
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 1.565
RIV identification code RIV/00216224:14330/02:00005604
Organization unit Faculty of Informatics
UT WoS 000176344700015
Keywords in English cytokinins; Dianthus caryophyllus; 4-pu-30; thidiazuron; photosystem II; chlorophyll fluorescence; delayed fluorescence; heat-induced changes; gas-exchange; photosynthetic response; cytokinin activity; high-temperature; plant-growth; in-vitro; accumulation; adaptation; cotyledons.
Tags 4-PU-30, accumulation, adaptation, chlorophyll fluorescence, cotyledons., cytokinin activity, cytokinins, delayed fluorescence, Dianthus caryophyllus, gas-exchange, heat-induced changes, high-temperature, in-vitro, photosynthetic response, photosystem II, plant-growth, thidiazuron
Tags International impact, Reviewed
Changed by Changed by: Ing. Matej Lexa, Ph.D., učo 31298. Changed: 21/11/2006 21:16.
Abstract
Using a novel system for expressing ipt gene from Agrobacterium tumefaciens in tobacco (Nicotiana tabacum L., cv. Petit Havana SR1), we were able to grow seedlings and teratoma-like tissue with increased content of cytokinins. This material enabled us to investigate new regulatory aspects of nitrate reduction. We had grown control plants and plants with elevated cytokinins on MS media with or without nitrate and benzyladenine (BA). We determined in vitro nitrate reductase (E.C. 1.6.6.1) activity (NRA) in this plant material. Initially, we found that ipt-expressing plants always displayed lowered levels of NRA when compared to wild-type SR1 plants. We determined that long-term exposure of tobacco plants and tissue to cytokinins caused up to 60% decrease in NRA. Exposure to 40 mM nitrate was able to induce the activity in such plants 3-fold, increasing the activity in SR1 plants more than 5-fold. We were able to restore wild-type levels of NRA in ipt-expressing plants by simultaneous induction of NR with BA and nitrate. Our results suggest that regulation of NR by nitrate and cytokinin is a result of overlaying cytokinin-driven regulatory processes, with those acting in short-term having positive effect on NRA and those acting over extended periods of time having inhibitory effects on NRA.
Links
IAA5004001, research and development projectName: Systém aktivace transkripce pro studium vztahu mezi metabolizmem a účinkem cytokininů u Arabidopsis a tabáku
Investor: Academy of Sciences of the Czech Republic, Grants of distinctly investigative character focused on the sphere of research pursued at present particularly in the ASCR
MSM 143100008, plan (intention)Name: Genomy a jejich funkce
Investor: Ministry of Education, Youth and Sports of the CR, Research Intents
VS96096, research and development projectName: Laboratoř molekulární fyziologie rostlin
Investor: Ministry of Education, Youth and Sports of the CR, Support to University Research Programmes
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