Immunohistochemical study of the extracellular matrix formed
during peripheral nerve regeneration through a knitted
prosthesis

J 2001

Immunohistochemical study of the extracellular matrix formed during peripheral nerve regeneration through a knitted prosthesis

SVÍŽENSKÁ, Ivana a Petr DUBOVÝ

Základní údaje

Originální název

Immunohistochemical study of the extracellular matrix formed during peripheral nerve regeneration through a knitted prosthesis

Název česky

Imunohistochemická studie ECM během regenerace periferního nervu přes pletenou protézu

Autoři

SVÍŽENSKÁ, Ivana a Petr DUBOVÝ

Vydání

Scripta medica, Brno, Masarykova univerzita, Lékařská fakulta, 2001, 1211-3395

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30000 3. Medical and Health Sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14110/01:00005656

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

Regeneration; Peripheral nerve; Extracellular matrix; Immunohistochemistry

Štítky

extracellular matrix, Immunohistochemistry, Peripheral nerve, Regeneration

Změněno: 17. 6. 2009 11:10, prof. RNDr. Petr Dubový, CSc.

Anotace

ORIG CZ

V originále

The extracellular matrix formed in a knitted prosthesis, which was applied between the stumps of a transected sciatic nerve of the rat, was investigated to find out whether it provided suitable conditions for nerve regeneration. After 6 weeks, the prosthesis contained a nerve outgrowth connecting the nerve stumps. The immunohistochemical staining of the cryostat sections through the middle of the nerve prosthesis revealed that thrombospondin, fibronectin, chondroitin sulfate A and C, and type IV collagen were localised in the connective tissue surrounding the nerve outgrowth, in the perineurium of individual minifascicles, as well as in the endoneurium among regenerated nerve fibres. No immunostaining for vitronectin was observed in cross-sections through the nerve outgrowth. Our results suggest that the environment formed during nerve regrowth within a knitted prosthesis provides conditions supporting the regeneration of nerve fibres.

Česky

Extracelulární matrix je tvořena podél regenerujících axonů v pletené nervové protéze.

Návaznosti

GA309/00/0407, projekt VaV

Název: Celulární a extracelulární prostředí pro regeneraci axonů při kořenových a distálních lezích periferních nervů

Investor: Grantová agentura ČR, Celulární a extracelulární prostředí pro regeneraci axonů při kořenových a distálních lezích periferních nervů

Citovat

SVÍŽENSKÁ, Ivana a Petr DUBOVÝ. Immunohistochemical study of the extracellular matrix formed during peripheral nerve regeneration through a knitted prosthesis. Scripta medica. Brno: Masarykova univerzita, Lékařská fakulta, 2001, roč. 74, č. 3, s. 221-230. ISSN 1211-3395.

@article{403231,
   author = {Svíženská, Ivana and Dubový, Petr},
   article_location = {Brno},
   article_number = {3},
   keywords = {Regeneration; Peripheral nerve; Extracellular matrix; Immunohistochemistry},
   language = {eng},
   issn = {1211-3395},
   journal = {Scripta medica},
   title = {Immunohistochemical study of the extracellular matrix formed during peripheral nerve regeneration through a knitted prosthesis},
   volume = {74},
   year = {2001}
}

TY  - JOUR
ID  - 403231
AU  - Svíženská, Ivana - Dubový, Petr
PY  - 2001
TI  - Immunohistochemical study of the extracellular matrix formed during peripheral nerve regeneration through a knitted prosthesis
JF  - Scripta medica
VL  - 74
IS  - 3
SP  - 221
EP  - 221
PB  - Masarykova univerzita, Lékařská fakulta
SN  - 12113395
KW  - Regeneration
KW  - Peripheral nerve
KW  - Extracellular matrix
KW  - Immunohistochemistry
N2  - The extracellular matrix formed in a knitted prosthesis, which was applied between the stumps of a transected sciatic nerve of the rat, was investigated to find out whether it provided suitable conditions for nerve regeneration. After 6 weeks, the prosthesis contained a nerve outgrowth connecting the nerve stumps. The immunohistochemical staining of the cryostat sections through the middle of the nerve prosthesis revealed that thrombospondin, fibronectin, chondroitin sulfate A and C, and type IV collagen were localised in the connective tissue surrounding the nerve outgrowth, in the perineurium of individual minifascicles, as well as in the endoneurium among regenerated nerve fibres. No immunostaining for vitronectin was observed in cross-sections through the nerve outgrowth. Our results suggest that the environment formed during nerve regrowth within a knitted prosthesis provides conditions supporting the regeneration of nerve fibres.
ER  -

SVÍŽENSKÁ, Ivana a Petr DUBOVÝ. Immunohistochemical study of the extracellular matrix formed during peripheral nerve regeneration through a knitted prosthesis. \textit{Scripta medica}. Brno: Masarykova univerzita, Lékařská fakulta, 2001, roč.~74, č.~3, s.~221-230. ISSN~1211-3395.

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