Specific thiol determination by on-column derivatization during
MEKC

D 2002

Specific thiol determination by on-column derivatization during MEKC

ŠEVČÍKOVÁ, Petra a Zdeněk GLATZ

Základní údaje

Originální název

Specific thiol determination by on-column derivatization during MEKC

Autoři

ŠEVČÍKOVÁ, Petra a Zdeněk GLATZ

Vydání

2002. vyd. Brno, Book of abstracts of 6th Meeting of biochemists and molecular biologists, s. 75-75, 2002

Nakladatel

MU Brno

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

thiol; MEKC

Štítky

MEKC, thiol

Změněno: 19. 5. 2009 18:44, prof. RNDr. Zdeněk Glatz, CSc.

Anotace

V originále

The latest development in derivatization in capillary electrophoresis is on-column mode. In this set-up a derivatization reaction is performed during the electrophoretic separation. In principle, the front end of the capillary is used as the reaction chamber. The analyte and the reagent are injected separately and are mixed because of their different electrophoretic mobilities. Various on-column reactions have been demonstrated so far however only for derivatization of the amino group containing compounds. In all these applications the simplified derivatization scheme is the same. The detector nonresponding analytes A, B and C are converted into the derivatives AX, BX and CX that have enhanced detector response properties, in the chemical reaction with the derivatization reagent X : A + B + C + .... + X Ž AX + BX + CX + .... In this study a new type of on-column reaction detection is reported. The analytes A, B, and C react with the reagent XX forming the derivatives AX, BX and CX, and a reaction by-product X. In contrast to the methods mentioned above, this highly absorbing by-product X is used for the detection : A + B + C + .... + XX Ž AX + BX + CX + .... n X Considering that the by-product X is identical for all analytes, the separation of the analytes has to be performed before the reaction. As an example of the utility of this concept the thiol-disulfide exchange reaction of 2,2-dipyridyldisulphide DPDS was used for detection of thiol RSH.

Návaznosti

GA525/00/0785, projekt VaV

Název: Acidifikační procesy v sulfidových odpadech

Investor: Grantová agentura ČR, Acidifikační procesy v sulfidových odpadech

Citovat

ŠEVČÍKOVÁ, Petra a Zdeněk GLATZ. Specific thiol determination by on-column derivatization during MEKC. In Book of abstracts of 6th Meeting of biochemists and molecular biologists. 2002. vyd. Brno: MU Brno, 2002, s. 75.

@inproceedings{403281,
   author = {Ševčíková, Petra and Glatz, Zdeněk},
   address = {Brno},
   booktitle = {Book of abstracts of 6th Meeting of biochemists and molecular biologists},
   edition = {2002},
   keywords = {thiol; MEKC},
   language = {eng},
   location = {Brno},
   pages = {75-75},
   publisher = {MU Brno},
   title = {Specific thiol determination by on-column derivatization during MEKC},
   year = {2002}
}

TY  - CONF
ID  - 403281
AU  - Ševčíková, Petra - Glatz, Zdeněk
PY  - 2002
TI  - Specific thiol determination by on-column derivatization during MEKC
PB  - MU Brno
CY  - Brno
KW  - thiol
KW  - MEKC
N2  - The latest development in derivatization in capillary electrophoresis is on-column mode. In this set-up a derivatization reaction is performed during the electrophoretic separation. In principle, the front end of the capillary is used as the reaction chamber. The analyte and the reagent are injected separately and are mixed because of their different electrophoretic mobilities. Various on-column reactions have been demonstrated so far however only for derivatization of the amino group containing compounds. In all these applications the simplified derivatization scheme is the same. The detector nonresponding analytes A, B and C are converted into the derivatives AX, BX and CX that have enhanced detector response properties, in the chemical reaction with the derivatization reagent X : A + B + C + .... + X Ž AX + BX + CX + .... In this study a new type of on-column reaction detection is reported. The analytes A, B, and C react with the reagent XX forming the derivatives AX, BX and CX, and a reaction by-product X. In contrast to the methods mentioned above, this highly absorbing by-product X is used for the detection : A + B + C + .... + XX Ž AX + BX + CX + .... n X Considering that the by-product X is identical for all analytes, the separation of the analytes has to be performed before the reaction. As an example of the utility of this concept the thiol-disulfide exchange reaction of 2,2-dipyridyldisulphide DPDS was used for detection of thiol RSH.
ER  -

ŠEVČÍKOVÁ, Petra a Zdeněk GLATZ. Specific thiol determination by on-column derivatization during MEKC. In \textit{Book of abstracts of 6th Meeting of biochemists and molecular biologists}. 2002. vyd. Brno: MU Brno, 2002, s.~75.

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