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@article{403459,
author = {Izakovičová Hollá, Lydie and Bučková, Dana and Kuhrová, Viera and Stejskalová, Andrea and Francová, Hana and Znojil, Vladimír and Vácha, Jirí},
article_location = {England},
article_number = {8},
keywords = {nitric oxide synthase; gene; polymorphism; asthma; allergic disease},
language = {eng},
issn = {0954-7894},
journal = {Clin Exp Allergy},
title = {Prevalence of endothelial nitric oxide synthase gene polymorphisms in patients with atopic asthma},
volume = {32},
year = {2002}
}
TY - JOUR
ID - 403459
AU - Izakovičová Hollá, Lydie - Bučková, Dana - Kuhrová, Viera - Stejskalová, Andrea - Francová, Hana - Znojil, Vladimír - Vácha, Jirí
PY - 2002
TI - Prevalence of endothelial nitric oxide synthase gene polymorphisms in patients with atopic asthma
JF - Clin Exp Allergy
VL - 32
IS - 8
SP - 1193
EP - 1193
PB - Blackwell Science
SN - 09547894
KW - nitric oxide synthase
KW - gene
KW - polymorphism
KW - asthma
KW - allergic disease
N2 - Asthma is a common multifactorial disease, the etiology of which is attributable to both environmental and genetic factors. The endothelial nitric oxide synthase (NOS3) gene has been implicated in asthma pathogenesis.This study investigated associations of 27 base-pair tandem repeat polymorphism in intron 4 and the Glu298Asp (G894T) variant of the NOS3 gene with atopic asthma in a Czech population. Polymerase chain reaction was used to determine the NOS3 genotypes in subjects with atopic asthma (n=163) and random controls (n=209). The NOS3 allele or genotype distributions did not significantly differ between the control and asthma groups. However, the common genotype (bb) of the NOS3 polymorphism in intron 4 was found to be significantly associated with total IgE levels (P=0.006), specific IgE levels for feathers (p=0.0002) and a positive skin prick test for hay (P=0.004). In one atopic patient, we identified an additional 27-bp repeat in the NOS3 gene (NOS3c), which occurred in heterozygous combination with the NOS3b allele (NOS3b/c genotype). In addition, we describe a new polymorphism (A5495G) in the NOS3 gene, which was in almost complete linkage disequilibrium with the NOS3 repeat polymorphism in intron 4. The Glu298Asp variant was not associated with asthma and/or related atopic phenotypes in our study. Neither the NOS3 b allele nor the NOS3 b/b genotype showed any general association with atopic asthma, but they were associated with atopy-related phenotypes. We conclude that the NOS3 gene polymorphisms may act as disease modifiers in atopic asthma phenotype in our population.
ER -
IZAKOVIČOVÁ HOLLÁ, Lydie, Dana BUČKOVÁ, Viera KUHROVÁ, Andrea STEJSKALOVÁ, Hana FRANCOVÁ, Vladimír ZNOJIL and Jirí VÁCHA. Prevalence of endothelial nitric oxide synthase gene polymorphisms in patients with atopic asthma. \textit{Clin Exp Allergy}. England: Blackwell Science, 2002, vol.~32, No~8, p.~1193-1198. ISSN~0954-7894.
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