Catalytic Signal of Rabbit Liver Metallothionein on a Mercury
Electrode:A Combination of Derivative Chronopotentiometry with
Adsorptive Transfer Stripping

J 2002

Catalytic Signal of Rabbit Liver Metallothionein on a Mercury Electrode:A Combination of Derivative Chronopotentiometry with Adsorptive Transfer Stripping

TRNKOVÁ, Libuše, René KIZEK a Jan VACEK

Základní údaje

Originální název

Catalytic Signal of Rabbit Liver Metallothionein on a Mercury Electrode:A Combination of Derivative Chronopotentiometry with Adsorptive Transfer Stripping

Autoři

TRNKOVÁ, Libuše (203 Česká republika, garant), René KIZEK (203 Česká republika) a Jan VACEK (203 Česká republika)

Vydání

Bioelectrochemistry, The Netherlands, Elsevier, 2002, 1567-5394

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10405 Electrochemistry

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.463

Kód RIV

RIV/00216224:14310/02:00005813

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Metallothionein;Chronopotentiometric stripping analysis;Adsorptive transfer stripping;Peak H;Catalytic hydrogen evolution;Effect of aeration

Štítky

adsorptive transfer stripping, catalytic hydrogen evolution, Chronopotentiometric stripping analysis, Effect of aeration, metallothionein, Peak H

Změněno: 24. 5. 2002 13:49, prof. RNDr. Libuše Trnková, CSc.

Anotace

V originále

Constant current chronopotentiometric stripping analysis (CPSA) in combination with adsorptive transfer stripping (AdTS) technique was used to study the rabbit liver metallothionein (MT) on a hanging mercury drop electrode (HMDE). Metallothionein yielded a distinct, sharp chronopotentiometric signal at very negative potentials (about -1.7 V ), also known as the "peak H". The height and potential of this peak were dependent on experimental conditions, such as buffer composition, pH, and the presence oxygen in solutions. The peak H was highest in borate buffer with pH close to the isoelectric point (pI) of MT. The chronopotentiometric results contribute to a deeper understanding of the nature of catalytic hydrogen evolution and demonstrate the usefulness of the peak H in peptide and protein research.

Návaznosti

GV204/97/K084, projekt VaV

Název: Elektrody modifikované nukleovými kyselinami a bílkovinami. Nové nástroje v biochemickém a biomedicínském výzkumu

Investor: Grantová agentura ČR, Elektrody modifikované nukleovými kyselinami a bílkovinami. Nové nástroje v biochemickém a biomedicínském výzkumu

MSM 143100011, záměr

Název: Struktura a vazebné poměry, vlastnosti a analýza syntetických a přírodních molekulových ansamblů

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Struktura a vazebné poměry, vlastnosti a analýza syntetických a přírodních molekulových ansamblů

Citovat

TRNKOVÁ, Libuše, René KIZEK a Jan VACEK. Catalytic Signal of Rabbit Liver Metallothionein on a Mercury Electrode:A Combination of Derivative Chronopotentiometry with Adsorptive Transfer Stripping. Bioelectrochemistry. The Netherlands: Elsevier, 2002, roč. 3, č. 56, s. 57-61. ISSN 1567-5394.

@article{403896,
   author = {Trnková, Libuše and Kizek, René and Vacek, Jan},
   article_location = {The Netherlands},
   article_number = {56},
   keywords = {Metallothionein;Chronopotentiometric stripping analysis;Adsorptive transfer stripping;Peak H;Catalytic hydrogen evolution;Effect of aeration},
   language = {eng},
   issn = {1567-5394},
   journal = {Bioelectrochemistry},
   title = {Catalytic Signal of Rabbit Liver Metallothionein on a Mercury Electrode:A Combination of Derivative Chronopotentiometry with Adsorptive Transfer Stripping},
   volume = {3},
   year = {2002}
}

TY  - JOUR
ID  - 403896
AU  - Trnková, Libuše - Kizek, René - Vacek, Jan
PY  - 2002
TI  - Catalytic Signal of Rabbit Liver Metallothionein on a Mercury Electrode:A Combination of Derivative Chronopotentiometry with Adsorptive Transfer Stripping
JF  - Bioelectrochemistry
VL  - 3
IS  - 56
SP  - 57
EP  - 57
PB  - Elsevier
SN  - 15675394
KW  - Metallothionein;Chronopotentiometric stripping analysis;Adsorptive transfer stripping;Peak H;Catalytic hydrogen evolution;Effect of aeration
N2  - Constant current chronopotentiometric stripping analysis (CPSA) in combination with adsorptive transfer stripping (AdTS) technique was used to study the rabbit liver metallothionein (MT) on a hanging mercury drop electrode (HMDE). Metallothionein yielded a distinct, sharp chronopotentiometric signal at very negative potentials (about -1.7 V ), also known as the "peak H". The height and potential of this peak were dependent on experimental conditions, such as buffer composition, pH, and the presence oxygen in solutions. The peak H was highest in borate buffer with pH close to the isoelectric point (pI) of MT. The chronopotentiometric results contribute to a deeper understanding of the nature of catalytic hydrogen evolution and demonstrate the usefulness of the peak H in peptide and protein research.
ER  -

TRNKOVÁ, Libuše, René KIZEK a Jan VACEK. Catalytic Signal of Rabbit Liver Metallothionein on a Mercury Electrode:A Combination of Derivative Chronopotentiometry with Adsorptive Transfer Stripping. \textit{Bioelectrochemistry}. The Netherlands: Elsevier, 2002, roč.~3, č.~56, s.~57-61. ISSN~1567-5394.

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