Use of artificial neural networks for the evaluation UV-Vis spectra of adenine and cytosine in mixtures

TOPINKOVÁ, Jana; Libuše TRNKOVÁ and Josef HAVEL. Use of artificial neural networks for the evaluation UV-Vis spectra of adenine and cytosine in mixtures. In Chemické listy. Praha: Česká společnost chemická, 2002, p. 482. ISBN 0009-2770.

Basic information

Original name

Use of artificial neural networks for the evaluation UV-Vis spectra of adenine and cytosine in mixtures

Authors

TOPINKOVÁ, Jana (203 Czech Republic); Libuše TRNKOVÁ (203 Czech Republic, guarantor) and Josef HAVEL (203 Czech Republic)

Edition

Praha, Chemické listy, p. 482-482, 2002

Publisher

Česká společnost chemická

---

Other information

Language

English

Type of outcome

Proceedings paper

Field of Study

10406 Analytical chemistry

Country of publisher

Czech Republic

Confidentiality degree

is not subject to a state or trade secret

RIV identification code

RIV/00216224:14310/02:00006278

Organization unit

Faculty of Science

ISBN

0009-2770

Keywords in English

adenine;cytosine;UV-Vis spectra;artificial neural networks;TRAJAN 6;chemometrics

Tags

adenine, artificial neural networks, chemometrics, cytosine, TRAJAN 6, UV-Vis spectra

---

Abstract

In the original language

Recently we have demonstrated advantages of artificial neural networks (ANNs) for quantification of nucleic acid bases mixtures from overlapping voltammetric signals. In this work ANNs were applied to analyze adenine-cytosine mixtures from UV-Vis spectra. Spectra of these compounds are highly similar (lambda max 261 and 270nm, respectively) and common spectral approaches of analysis are failing. The optimal ANN architecture, able to model the system, was established. Software of ANNs method was from TRAJAN 6 program. Using the suitable experimental design with soft ANN modeling adenine - cytosine mixtures can be quantified with an excellent precision (less than 1%). Generally, ANNs open many possibilities for the analysis of mixtures of biologically important substances with very overlapped signals, even without the explicit knowledge of their behavior.

---

Links

GA203/02/0422, research and development project	Name: Nové směry v elektrochemii nukleových kyselin a jejich aplikace v chemii životního prostředí Investor: Czech Science Foundation, New trends in electrochemistry of nucleic acids and their applications in enviromental chemistry
GV204/97/K084, research and development project	Name: Elektrody modifikované nukleovými kyselinami a bílkovinami. Nové nástroje v biochemickém a biomedicínském výzkumu Investor: Czech Science Foundation, Electrodes modified with nucleic acids and proteins. New tools in biochemical and biomedical research
IAA1163201, research and development project	Name: Využití adsorptivní přenosové a eliminační techniky pro elektrochemickou analýzu oligonukleotidů a nukleových kyselin Investor: Academy of Sciences of the Czech Republic, Application of adsorptive transfer and elemination techniques in oligonucleotides and nucleic acids analysis.
MSM 143100011, plan (intention)	Name: Struktura a vazebné poměry, vlastnosti a analýza syntetických a přírodních molekulových ansamblů Investor: Ministry of Education, Youth and Sports of the CR, Structure and character of bonding, properties and analysis of synthetic and natural molecular ensembles