Determination of Selenium in Blood Serum by Inductively Coupled
Plasma Atomic Emission Spectrometry with Pneumatic Nebulization

J 2002

Determination of Selenium in Blood Serum by Inductively Coupled Plasma Atomic Emission Spectrometry with Pneumatic Nebulization

MACHÁT, Jiří; Viktor KANICKÝ a Vítězslav OTRUBA

Základní údaje

Originální název

Determination of Selenium in Blood Serum by Inductively Coupled Plasma Atomic Emission Spectrometry with Pneumatic Nebulization

Autoři

MACHÁT, Jiří; Viktor KANICKÝ a Vítězslav OTRUBA

Vydání

Analytical and Bioanalytical Chemistry, Heidelberg, Springer, 2002, 1618-2642

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/02:00006344

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000176103100012

Klíčová slova anglicky

Selenium speciation; Spectrometry; ICP-AES; Pneumatic nebulization

Štítky

ICP-AES, Pneumatic nebulization, Selenium speciation, Spectrometry

Změněno: 20. 5. 2003 14:53, Mgr. Jiří Machát, Ph.D.

Anotace

V originále

The possibility of determination of selenium in blood serum using inductively coupled plasma emission spectrometry with conventional pneumatic nebulization was studied. A high-resolution spectrometer (SBW = 6 pm) with laterally viewed ICP was employed. Analysis with conventional pneumatic nebulization could overcome laborious and demanding digestion, which is necessary for hydride generation. A pressure digestion with nitric acid at 160oC was sufficient to decrease the carbon content in serum sample to 5-10% of its original value. Spectral interference of the CN band was observed and mathematically corrected. It was found that the carbon-induced selenium line emission enhancement occurred even under ICP optimized conditions. A method of determination was developed and applied to the analysis of blood serum. True limit of detection in real samples is 0.01-0.02 mg/L and the limit of quantification (RSD 10%) is 0.03-0.07 mg/L using Se I 196.090 nm line at an integration time 10-2 s. The method was tested by analysis of porcine blood serum and serum reference material Seronorm MI 0181.

Návaznosti

MSM 143100003, záměr

Název: Studium plazmochemických reakcí v neizotermickém nízkoteplotním plazmatu a jeho interakcí s povrchem pevných látek

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Studium plazmochemických reakcí v neizotermickém nízkoteplotním plazmatu a jeho interakcí s povrchem pevných látek

Citovat

MACHÁT, Jiří; Viktor KANICKÝ a Vítězslav OTRUBA. Determination of Selenium in Blood Serum by Inductively Coupled Plasma Atomic Emission Spectrometry with Pneumatic Nebulization. Analytical and Bioanalytical Chemistry. Heidelberg: Springer, 2002, roč. 372, č. 4, s. 576-581. ISSN 1618-2642.

@article{405042,
   author = {Machát, Jiří and Kanický, Viktor and Otruba, Vítězslav},
   article_location = {Heidelberg},
   article_number = {4},
   keywords = {Selenium speciation; Spectrometry; ICP-AES; Pneumatic nebulization},
   language = {eng},
   issn = {1618-2642},
   journal = {Analytical and Bioanalytical Chemistry},
   title = {Determination of Selenium in Blood Serum by Inductively Coupled Plasma Atomic Emission Spectrometry with Pneumatic Nebulization},
   volume = {372},
   year = {2002}
}

TY  - JOUR
ID  - 405042
AU  - Machát, Jiří - Kanický, Viktor - Otruba, Vítězslav
PY  - 2002
TI  - Determination of Selenium in Blood Serum by Inductively Coupled Plasma Atomic Emission Spectrometry with Pneumatic Nebulization
JF  - Analytical and Bioanalytical Chemistry
VL  - 372
IS  - 4
SP  - 576
EP  - 576
PB  - Springer
SN  - 16182642
KW  - Selenium speciation
KW  - Spectrometry
KW  - ICP-AES
KW  - Pneumatic nebulization
N2  - The possibility of determination of selenium in blood serum using inductively coupled plasma emission spectrometry with conventional pneumatic nebulization was studied. A high-resolution spectrometer (SBW = 6 pm) with laterally viewed ICP was employed. Analysis with conventional pneumatic nebulization could overcome laborious and demanding digestion, which is necessary for hydride generation. A pressure digestion with nitric acid at 160oC was sufficient to decrease the carbon content in serum sample to 5-10% of its original value. Spectral interference of the CN band was observed and mathematically corrected. It was found that the carbon-induced selenium line emission enhancement occurred even under ICP optimized conditions. A method of determination was developed and applied to the analysis of blood serum. True limit of detection in real samples is 0.01-0.02 mg/L and the limit of quantification (RSD 10%) is 0.03-0.07 mg/L using Se I 196.090 nm line at an integration time 10-2 s. The method was tested by analysis of porcine blood serum and serum reference material Seronorm MI 0181.
ER  -

MACHÁT, Jiří; Viktor KANICKÝ a Vítězslav OTRUBA. Determination of Selenium in Blood Serum by Inductively Coupled Plasma Atomic Emission Spectrometry with Pneumatic Nebulization. \textit{Analytical and Bioanalytical Chemistry}. Heidelberg: Springer, 2002, roč.~372, č.~4, s.~576-581. ISSN~1618-2642.

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