The microbicidal effect of the silkworm(Bombyx mori)hemocytes
is not dependent on oxygen reactive metabolites

D 2002

The microbicidal effect of the silkworm(Bombyx mori)hemocytes is not dependent on oxygen reactive metabolites

HYRŠL, Pavel; Antonín LOJEK; Milan ČÍŽ a Lukáš KUBALA

Základní údaje

Originální název

The microbicidal effect of the silkworm(Bombyx mori)hemocytes is not dependent on oxygen reactive metabolites

Název anglicky

The microbicidal effect of the silkworm(Bombyx mori)hemocytes is not dependent on oxygen reactive metaboliteson

Autoři

HYRŠL, Pavel; Antonín LOJEK; Milan ČÍŽ a Lukáš KUBALA

Vydání

Bratislava, Zborník XVIII. Biochemický zjazd, s. 204-204, 2002

Nakladatel

VEDA

Další údaje

Typ výsledku

Stať ve sborníku

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Bombyx mori; hemocytes; oxygen metabolites

Štítky

Bombyx mori, hemocytes, oxygen metabolites

Změněno: 20. 3. 2006 15:17, doc. RNDr. Pavel Hyršl, Ph.D.

Anotace

V originále

Hemocytes belong to the basic elements of invertebrate immune system. Increased reactive oxygen metabolites (ROM) generation was found in activated molluscan hemocytes (LAMBERT & NICOLAS, 1998; ORDAS et al. 2000), and therefore ROM are considered to be a significant microbicidal factor of invertebrate hemocytes similarly as of vertebrate phagocytes. The aim of this study was to analyse the ROM production of silkworm (Bombyx mori) hemocytes using various methods. Hemocytes were isolated from hemolymph of polyvoltine hybrid NO2CO2. Spontaneous chemiluminescence (CL) of hemocytes was at the background level. In following experiments hemocytes were activated by phorbol myristate acetate, zymosan particles or calcium ionophore A23187. The increase in CL was observed neither in this set of experiments. ROM production was also observed using spectrophotometric (NBT test, cytochrome c assay) and flow cytometric (dihydrorhodamine and hydroethidine staining) analyses. Again, no ROM production was observed. Similar results with CL method are also referred by other authors (e.g. MAZET et al., 1994) for other Lepidoptera species. In conclusion, no microbicidal activity dependent on oxygen was found in silkworm hemocytes. It is likely that insect hemocytes react differently in comparison with other invertebrate hemocytes and mammalian phagocytes and do not employ ROM to destroy invading microorganisms. References: LAMBERT, C., NICOLAS, J. L. 1998. J. Invertebr. Pathol. 71 (1). 53-63. MAZET, I., PENDLAND, J., BOUCIAS, D. 1994. Dev. Comp. Immunol. 18 (6). 455-466. ORDAS, M. C., NOVOA, B., FIGUERAS, A. 2000. Fish Shellfish Immunol. 10 (7). 611-622.

Citovat

HYRŠL, Pavel; Antonín LOJEK; Milan ČÍŽ a Lukáš KUBALA. The microbicidal effect of the silkworm(Bombyx mori)hemocytes is not dependent on oxygen reactive metabolites. In Zborník XVIII. Biochemický zjazd. Bratislava: VEDA, 2002, s. 204.

@inproceedings{405419,
   author = {Hyršl, Pavel and Lojek, Antonín and Číž, Milan and Kubala, Lukáš},
   address = {Bratislava},
   booktitle = {Zborník XVIII. Biochemický zjazd},
   keywords = {Bombyx mori; hemocytes; oxygen metabolites},
   location = {Bratislava},
   pages = {204-204},
   publisher = {VEDA},
   title = {The microbicidal effect of the silkworm(Bombyx mori)hemocytes is not dependent on oxygen reactive metabolites},
   year = {2002}
}

TY  - CONF
ID  - 405419
AU  - Hyršl, Pavel - Lojek, Antonín - Číž, Milan - Kubala, Lukáš
PY  - 2002
TI  - The microbicidal effect of the silkworm(Bombyx mori)hemocytes is not dependent on oxygen reactive metabolites
PB  - VEDA
CY  - Bratislava
KW  - Bombyx mori
KW  - hemocytes
KW  - oxygen metabolites
N2  - Hemocytes belong to the basic elements of invertebrate immune system. Increased reactive oxygen metabolites (ROM) generation was found in activated molluscan hemocytes (LAMBERT & NICOLAS, 1998; ORDAS et al. 2000), and therefore ROM are considered to be a significant microbicidal factor of invertebrate hemocytes similarly as of vertebrate phagocytes. The aim of this study was to analyse the ROM production of silkworm (Bombyx mori) hemocytes using various methods. Hemocytes were isolated from hemolymph of polyvoltine hybrid NO2CO2. Spontaneous chemiluminescence (CL) of hemocytes was at the background level. In following experiments hemocytes were activated by phorbol myristate acetate, zymosan particles or calcium ionophore A23187. The increase in CL was observed neither in this set of experiments. ROM production was also observed using spectrophotometric (NBT test, cytochrome c assay) and flow cytometric (dihydrorhodamine and hydroethidine staining) analyses. Again, no ROM production was observed. Similar results with CL method are also referred by other authors (e.g. MAZET et al., 1994) for other Lepidoptera species. In conclusion, no microbicidal activity dependent on oxygen was found in silkworm hemocytes. It is likely that insect hemocytes react differently in comparison with other invertebrate hemocytes and mammalian phagocytes and do not employ ROM to destroy invading microorganisms. References: LAMBERT, C., NICOLAS, J. L. 1998. J. Invertebr. Pathol. 71 (1). 53-63. MAZET, I., PENDLAND, J., BOUCIAS, D. 1994. Dev. Comp. Immunol. 18 (6). 455-466. ORDAS, M. C., NOVOA, B., FIGUERAS, A. 2000. Fish Shellfish Immunol. 10 (7). 611-622.
ER  -

HYRŠL, Pavel; Antonín LOJEK; Milan ČÍŽ a Lukáš KUBALA. The microbicidal effect of the silkworm(Bombyx mori)hemocytes is not dependent on oxygen reactive metabolites. In \textit{Zborník XVIII. Biochemický zjazd}. Bratislava: VEDA, 2002, s.~204.

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