Use of molecular-biological methods and HPLC for discrimination of Armillaria species in Czech Republic

LOCHMAN, Jan, Omar ŠERÝ, Libor JANKOVSKÝ and Vladimír MIKEŠ. Use of molecular-biological methods and HPLC for discrimination of Armillaria species in Czech Republic. In Book of Abstracts of the congress EFPP. Prague: European Foundation for Plant Pathology, 2002, p. 7.

Basic information

• Original name: Use of molecular-biological methods and HPLC for discrimination of Armillaria species in Czech Republic
• Authors: LOCHMAN, Jan (203 Czech Republic, guarantor), Omar ŠERÝ (203 Czech Republic), Libor JANKOVSKÝ (203 Czech Republic) and Vladimír MIKEŠ (203 Czech Republic).
• Edition: Prague, Book of Abstracts of the congress EFPP, p. 7-7, 2002.
• Publisher: European Foundation for Plant Pathology

Other information

• Original language: English
• Type of outcome: Proceedings paper
• Field of Study: Genetics and molecular biology
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14310/02:00006494
• Organization unit: Faculty of Science
• Keywords in English: Armillaria; discrimination; ITS; PCR
• Tags: Armillaria, discrimination, ITS, PCR
• Tags: International impact

Abstract

More than 40 isolates containing six species of Armillaria from the collections have been analyzed (A. borealis, A. cepistipes, A. gallica, A. mellea, A. ostoyae and A. tabescens) using the PCR of the internal transcribed spacer (ITS). The amplificates were further analysed by RFLP (Alu I, Hinf I and Mbo I) and electrophoreticaly separated both on 3% agarose gel and by IEC- HPLC on TSKgel DEAE-NPR column (Supelco). Digestion with Hinf I allowed discrimination of all six species.

Links

• MSM 143100005, plan (intention): Name: Strukturně-funkční vztahy biomolekul a jejich role v metabolismu

Investor: Ministry of Education, Youth and Sports of the CR, Biomolecular Structure-function Relationships and their role in the Metabolism