Occurrence of antibiotic-resistant bacterial strains isolated
in poultry

J 2002

Occurrence of antibiotic-resistant bacterial strains isolated in poultry

KOLÁŘ, Milan, Roman PANTŮČEK, Jan BARDOŇ, Iva VÁGNEROVÁ, H. TYPOVSKÁ et. al.

Základní údaje

Originální název

Occurrence of antibiotic-resistant bacterial strains isolated in poultry

Autoři

KOLÁŘ, Milan (203 Česká republika), Roman PANTŮČEK (203 Česká republika, garant), Jan BARDOŇ (203 Česká republika), Iva VÁGNEROVÁ (203 Česká republika), H. TYPOVSKÁ (203 Česká republika), Ivo VÁLKA (203 Česká republika) a Jiří DOŠKAŘ (203 Česká republika)

Vydání

Veterinary Medicine - Czech, Brno, Veterinary Research Institute Brno, 2002, 0375-8427

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

[Full Text]

Impakt faktor

Impact factor: 0.204

Kód RIV

RIV/00216224:14310/02:00006631

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000175865800004

Klíčová slova anglicky

Vancomycin Resistance; Enterococcus; Staphylococcus; Pulsed Field-Gel Electrophoreris; Plasmid Analysis; Poultry

Štítky

Enterococcus, Plasmid Analysis, Poultry, Pulsed Field-Gel Electrophoreris, Staphylococcus, vancomycin resistance

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 1. 7. 2009 18:38, prof. RNDr. Roman Pantůček, Ph.D.

Anotace

V originále

The main goal of the study was to analyse the occurrence of antibiotic-resistant bacterial strains in poultry in the Czech Republic in 1999-2000. The resistance was determined in 128 selected Escherichia coli, 88 Staphylococcus sp. and 228 Enterococcus sp. strains. The bacterial species were selected to represent both gramnegatives and grampositives, both the common part of intestinal microflora and also opportunistic pathogens. In Escherichia coli, 97% of strains were found to be resistant to tetracycline, 51% were resistant to ampicillin, and 31% were resistant to piperacillin. Increased frequencies of resistance (in 10% of the strains) were found also against ofloxacin and ciprofloxacin. In staphylococci, increased numbers of strains resistant to erythromycin (39%), clindamycin (19%), tetracycline (14%) and ofloxacin (13%) were observed. In enterococci, 80%, 59% and 34% of the strains were resistant to tetracycline, erythromycin or nitrofurantoin, respectively. A high-level resistance to streptomycin was proved in 22% of the strains. Eleven Enterococcus sp. strains were found to be resistant against vancomycin (vancomycin-resistant enterococci - VRE). Being of the clinical importance, the VRE strains were analysed in detail. Six VRE were identified as Enterococcus faecium VanA, three strains belonged among Enterococcus sp. group III VanB. Remaining two strains were classified as Enterococcus faecium VanB and Enterococcus faecalis VanB, respectively. Based on SmaI macrorestriction analysis, vancomycin-resistant Enterococcus faecium strains, regardless of their resistance type, formed a cluster distinct from control group of vancomycin sensitive strains. Furthermore, among the cluster of vancomycin resistant Enterococcus faecium strains, two clonal lines could be distinguished while the sensitive strains were more heterogenous.

Návaznosti

GA301/99/D075, projekt VaV

Název: Genotypová diagnostika a typizace klinicky významných koaguláza negativních stafylokoků

Investor: Grantová agentura ČR, Genotypová diagnostika a typizace klinicky významných koaguláza negativních stafylokoků

MSM 143100008, záměr

Název: Genomy a jejich funkce

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Genomy a jejich funkce

Citovat

KOLÁŘ, Milan, Roman PANTŮČEK, Jan BARDOŇ, Iva VÁGNEROVÁ, H. TYPOVSKÁ, Ivo VÁLKA a Jiří DOŠKAŘ. Occurrence of antibiotic-resistant bacterial strains isolated in poultry. Veterinary Medicine - Czech. Brno: Veterinary Research Institute Brno, 2002, roč. 47, 2-3, s. 52-59. ISSN 0375-8427.

@article{406330,
   author = {Kolář, Milan and Pantůček, Roman and Bardoň, Jan and Vágnerová, Iva and Typovská, H. and Válka, Ivo and Doškař, Jiří},
   article_location = {Brno},
   article_number = {2-3},
   keywords = {Vancomycin Resistance; Enterococcus; Staphylococcus; Pulsed Field-Gel Electrophoreris; Plasmid Analysis; Poultry},
   language = {eng},
   issn = {0375-8427},
   journal = {Veterinary Medicine - Czech},
   title = {Occurrence of antibiotic-resistant bacterial strains isolated in poultry},
   url = {http://www.vri.cz/docs/vetmed/47-3-52.pdf},
   volume = {47},
   year = {2002}
}

TY  - JOUR
ID  - 406330
AU  - Kolář, Milan - Pantůček, Roman - Bardoň, Jan - Vágnerová, Iva - Typovská, H. - Válka, Ivo - Doškař, Jiří
PY  - 2002
TI  - Occurrence of antibiotic-resistant bacterial strains isolated in poultry
JF  - Veterinary Medicine - Czech
VL  - 47
IS  - 2-3
SP  - 52
EP  - 52
PB  - Veterinary Research Institute Brno
SN  - 03758427
KW  - Vancomycin Resistance
KW  - Enterococcus
KW  - Staphylococcus
KW  - Pulsed Field-Gel Electrophoreris
KW  - Plasmid Analysis
KW  - Poultry
UR  - http://www.vri.cz/docs/vetmed/47-3-52.pdf
N2  - The main goal of the study was to analyse the occurrence of antibiotic-resistant bacterial strains in poultry in the Czech Republic in 1999-2000. The resistance was determined in 128 selected Escherichia coli, 88 Staphylococcus sp. and 228 Enterococcus sp. strains. The bacterial species were selected to represent both gramnegatives and grampositives, both the common part of intestinal microflora and also opportunistic pathogens. In Escherichia coli, 97% of strains were found to be resistant to tetracycline, 51% were resistant to ampicillin, and 31% were resistant to piperacillin. Increased frequencies of resistance (in 10% of the strains) were found also against ofloxacin and ciprofloxacin. In staphylococci, increased numbers of strains resistant to erythromycin (39%), clindamycin (19%), tetracycline (14%) and ofloxacin (13%) were observed. In enterococci, 80%, 59% and 34% of the strains were resistant to tetracycline, erythromycin or nitrofurantoin, respectively. A high-level resistance to streptomycin was proved in 22% of the strains. Eleven Enterococcus sp. strains were found to be resistant against vancomycin (vancomycin-resistant enterococci - VRE). Being of the clinical importance, the VRE strains were analysed in detail. Six VRE were identified as Enterococcus faecium VanA, three strains belonged among Enterococcus sp. group III VanB. Remaining two strains were classified as Enterococcus faecium VanB and Enterococcus faecalis VanB, respectively. Based on SmaI macrorestriction analysis, vancomycin-resistant Enterococcus faecium strains, regardless of their resistance type, formed a cluster distinct from control group of vancomycin sensitive strains. Furthermore, among the cluster of vancomycin resistant Enterococcus faecium strains, two clonal lines could be distinguished while the sensitive strains were more heterogenous.
ER  -

KOLÁŘ, Milan, Roman PANTŮČEK, Jan BARDOŇ, Iva VÁGNEROVÁ, H. TYPOVSKÁ, Ivo VÁLKA a Jiří DOŠKAŘ. Occurrence of antibiotic-resistant bacterial strains isolated in poultry. \textit{Veterinary Medicine - Czech}. Brno: Veterinary Research Institute Brno, 2002, roč.~47, 2-3, s.~52-59. ISSN~0375-8427.

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