Multiplex PCR for detection of Staphylococcus aureus prophages

D 2002

Multiplex PCR for detection of Staphylococcus aureus prophages

PANTŮČEK, Roman, Eva ORÁČOVÁ, Veronika KVARDOVÁ, Vladislava RŮŽIČKOVÁ, Petr PETRÁŠ et. al.

Základní údaje

Originální název

Multiplex PCR for detection of Staphylococcus aureus prophages

Autoři

PANTŮČEK, Roman (203 Česká republika, garant), Eva ORÁČOVÁ (203 Česká republika), Veronika KVARDOVÁ (203 Česká republika), Vladislava RŮŽIČKOVÁ (203 Česká republika), Petr PETRÁŠ (203 Česká republika) a Jiří DOŠKAŘ (203 Česká republika)

Vydání

Tsukuba, Japan, 10th International Symposium on Staphylococci and Staphylococcal Infections, s. 66-66, 2002

Nakladatel

Juntendo University

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

Genetika a molekulární biologie

Stát vydavatele

Japonsko

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/02:00006633

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Bacterial Typing Techniques; DNA; Bacteriophages; Staphylococcus aureus; Lysogeny; Multiplex PCR; Prophage detection

Štítky

Bacterial Typing Techniques, bacteriophages, DNA, Lysogeny, Multiplex PCR, Prophage detection, Staphylococcus aureus

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 31. 10. 2008 15:02, prof. RNDr. Roman Pantůček, Ph.D.

Anotace

V originále

Background.Bacteriophages play an important part in gene transfer and gene expression in S.aureus strains and thus also in their virulence and antibiotic resistance.A set of primers for multiplex PCR was designed for identification of prophages of serogroups A,B,F and L in lysogenic and polylysogenic S.aureus strains. Methods.In the genomes of International Typing Set bacteriophages,the sequences specific for both the phage species and serogroups A (ö 3A),B (ö 53),F (ö 77)and L (ö 187)were estimated.The designed primers amplify specific genomic sequences of sizes characteristic of each of the phage species. The multiplex PCR specificity was verified on DNAs of 26 bacteriophages,9 lysogenized and 2 prophageless S.aureus strains. Results.One to three prophages of different serogroups were identified unambiguously in each of 40 clinical strains including 25 MRSA strains.Detectability of prophages by multiplex PCR is comparable with that by phage-specific probes prepared in our laboratory (Doskar et al.,2000,Can.J.Microbiol.46:1066-1076). Conclusions.In view of significant differences in the prophage content between S.aureus closely related isolates,prophage profiling is recommended as a highly sensitive method for strain discrimination.

Návaznosti

GA301/02/1505, projekt VaV

Název: Molekulární diagnostika, epidemiologie a klasifikace klinicky významných grampozitivních koků

Investor: Grantová agentura ČR, Molekulární diagnostika, epidemiologie a klasifikace klinicky významných grampozitivních koků

MSM 143100008, záměr

Název: Genomy a jejich funkce

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Genomy a jejich funkce

Citovat

PANTŮČEK, Roman, Eva ORÁČOVÁ, Veronika KVARDOVÁ, Vladislava RŮŽIČKOVÁ, Petr PETRÁŠ a Jiří DOŠKAŘ. Multiplex PCR for detection of Staphylococcus aureus prophages. In 10th International Symposium on Staphylococci and Staphylococcal Infections. Tsukuba, Japan: Juntendo University, 2002, s. 66.

@inproceedings{406333,
   author = {Pantůček, Roman and Oráčová, Eva and Kvardová, Veronika and Růžičková, Vladislava and Petráš, Petr and Doškař, Jiří},
   address = {Tsukuba, Japan},
   booktitle = {10th International Symposium on Staphylococci and Staphylococcal Infections},
   keywords = {Bacterial Typing Techniques; DNA; Bacteriophages; Staphylococcus aureus; Lysogeny; Multiplex PCR; Prophage detection},
   language = {eng},
   location = {Tsukuba, Japan},
   pages = {66-66},
   publisher = {Juntendo University},
   title = {Multiplex PCR for detection of Staphylococcus aureus prophages},
   year = {2002}
}

TY  - JOUR
ID  - 406333
AU  - Pantůček, Roman - Oráčová, Eva - Kvardová, Veronika - Růžičková, Vladislava - Petráš, Petr - Doškař, Jiří
PY  - 2002
TI  - Multiplex PCR for detection of Staphylococcus aureus prophages
PB  - Juntendo University
CY  - Tsukuba, Japan
KW  - Bacterial Typing Techniques
KW  - DNA
KW  - Bacteriophages
KW  - Staphylococcus aureus
KW  - Lysogeny
KW  - Multiplex PCR
KW  - Prophage detection
N2  - Background.Bacteriophages play an important part in gene transfer and gene expression in S.aureus strains and thus also in their virulence and antibiotic resistance.A set of primers for multiplex PCR was designed for identification of prophages of serogroups A,B,F and L in lysogenic and polylysogenic S.aureus strains. Methods.In the genomes of International Typing Set bacteriophages,the sequences specific for both the phage species and serogroups A (ö 3A),B (ö 53),F (ö 77)and L (ö 187)were estimated.The designed primers amplify specific genomic sequences of sizes characteristic of each of the phage species. The multiplex PCR specificity was verified on DNAs of 26 bacteriophages,9 lysogenized and 2 prophageless S.aureus strains. Results.One to three prophages of different serogroups were identified unambiguously in each of 40 clinical strains including 25 MRSA strains.Detectability of prophages by multiplex PCR is comparable with that by phage-specific probes prepared in our laboratory (Doskar et al.,2000,Can.J.Microbiol.46:1066-1076). Conclusions.In view of significant differences in the prophage content between S.aureus closely related isolates,prophage profiling is recommended as a highly sensitive method for strain discrimination.
ER  -

PANTŮČEK, Roman, Eva ORÁČOVÁ, Veronika KVARDOVÁ, Vladislava RŮŽIČKOVÁ, Petr PETRÁŠ a Jiří DOŠKAŘ. Multiplex PCR for detection of $<$I$>$Staphylococcus aureus$<$/I$>$ prophages. In \textit{10th International Symposium on Staphylococci and Staphylococcal Infections}. Tsukuba, Japan: Juntendo University, 2002, s.~66.

Podrobný výpis o publikaci