Detailed Information on Publication Record
2002
Characterization of deoxyribonuclease I immobilized on magnetic hydrophilic polymer particles
RITTICH, Bohuslav, Alena ŠPANOVÁ, Yuriy OHLASHENNYY, Jiří LENFELD, Ivo RUDOLF et. al.Basic information
Original name
Characterization of deoxyribonuclease I immobilized on magnetic hydrophilic polymer particles
Authors
RITTICH, Bohuslav (203 Czech Republic, guarantor), Alena ŠPANOVÁ (203 Czech Republic), Yuriy OHLASHENNYY (804 Ukraine), Jiří LENFELD (203 Czech Republic), Ivo RUDOLF (203 Czech Republic), Daniel HORÁK (203 Czech Republic) and Milan BENEŠ (203 Czech Republic)
Edition
Journal of Chromatography B, Amsterdam, Elsevier, 2002, 1570-0232
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
Biotechnology and bionics
Country of publisher
Netherlands
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 1.913
RIV identification code
RIV/00216224:14310/02:00006659
Organization unit
Faculty of Science
Keywords in English
Magnetic bead cellulose particles; magnetic poly(HEMA-co-EDMA); deoxyribonuclease I
Tags
Reviewed
Změněno: 21/11/2006 19:44, doc. Ing. Bohuslav Rittich, CSc.
Abstract
V originále
Magnetic bead cellulose particles and magnetic poly(HEMA-co-EDMA) microsheres with immobilized DNaseI were used for degradation of chromosomal and plasmid DNAs. Divalent cations (Mg2+, Ca2+, Mn2+ and Co2+) were used for the activation of DNaseI. A comparison of free and immobilized enzyme (magnetic bead particles) activities was carried out in dependence on pH and activating cation. The maximum of the activity of immobilized DNaseI was shifted to lower pH compared with free DNase I. DNase I immobilized on magnetic bead cellulose was used 20 times in the degradation of chromosomal DNA. Its residual activity was influenced by the nature of activating divalent cation. The immobilized enzyme with decreased activity was reactivated by Co2+ ions.
Links
GA203/00/1339, research and development project |
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GA203/98/1231, research and development project |
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