Detection of spectrin-like protein in yeasts: spectrin-actin
relations

D 2002

Detection of spectrin-like protein in yeasts: spectrin-actin relations

SLANINOVÁ, Iva, Alena HOLUBÁŘOVÁ a Augustin SVOBODA

Základní údaje

Originální název

Detection of spectrin-like protein in yeasts: spectrin-actin relations

Autoři

SLANINOVÁ, Iva (203 Česká republika, garant), Alena HOLUBÁŘOVÁ (203 Česká republika) a Augustin SVOBODA (203 Česká republika)

Vydání

Praha, Sborník X. Cytoskeletálního klubu, s. 14-14, 2002

Nakladatel

Československá biologická společnost

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14110/02:00006732

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

yeast; spectrin; cytoskeleton; actin

Štítky

Actin, Cytoskeleton, spectrin, Yeast

Změněno: 6. 5. 2003 16:22, prof. MUDr. Iva Slaninová, Ph.D.

Anotace

V originále

Proteins related to the a- and b- spectrin have been detected in almost all vertebrate tissues, and also in plants (1) and hyphal fungae (2). We studied spectrin-like proteins in budding yeasts Saccharomyces cerevisiae and fission yeasts Schizosaccharomyces japonicus var. versatilis. Western blot analysis using anti-chicken and anti-human erythrocyte spectrin antibodies detected band at 220 kDa in cells and protoplasts extracts of both yeast strains. Additional bands were present at 50, 34 and 20 kDa in S. cerevisiae, and 44, 34 and 20 kDa in Sch. versatilis. Fluorescence microscopy revealed distinct labelling of the spectrin-like antigens at the cell surfaces and in vacuolar membranes of cells and protoplasts of both yeast species. In addition, the antigens were spread in the cytoplasm of both cells as fluorescent patches and as a diffuse fluorescence in Sch. versatilis nuclei. In mating and sporulating cells spectrin-like antigens lined the cell surface and prespore membranes. Electron microscopy of immunogold labelled cells and protoplasts revealed spectrin-like antigens in the cell wall, plasma membrane, ER, vacuole, nuclei, vesicles and mitochondria. Contrary to plant and some other fungal cells, spectrin in yeasts did not co-localize with actin. Growing regions of the cells were not preferentially labelled. Spectrin antigens correlated with actin only in protoplasts and maturing spores where patches of both lined the whole surface. The topology of spectrin was not affected by actin depolymerisation with Latrunculin B, nor was it changed in either act1-1 or cdc42 mutants under restrictive conditions. Under osmotic stress, both spectrin and actin were delocalized and appeared in the form of large clusters in the cytoplasm. Our results confirmed the role for spectrin as a membrane protein and the fact that spectrin localization occurs independently of actin distribution.

Návaznosti

GA204/00/0394, projekt VaV

Název: Buněčná stěna kvasinek jako extracelulární matrix: cytoskelet a stěnové funkce

Investor: Grantová agentura ČR, Buněčná stěna kvasinek jako extracelulární matrix: cytoskelet a stěnové funkce

GA204/99/D025, projekt VaV

Název: Úloha cytoskeletu a buněčné stěny v polarizaci buňky

Investor: Grantová agentura ČR, Úloha cytoskeletu a buněčné stěny v polarizaci buňky

Citovat

SLANINOVÁ, Iva, Alena HOLUBÁŘOVÁ a Augustin SVOBODA. Detection of spectrin-like protein in yeasts: spectrin-actin relations. In Sborník X. Cytoskeletálního klubu. Praha: Československá biologická společnost, 2002, s. 14.

@inproceedings{406732,
   author = {Slaninová, Iva and Holubářová, Alena and Svoboda, Augustin},
   address = {Praha},
   booktitle = {Sborník X. Cytoskeletálního klubu},
   keywords = {yeast; spectrin; cytoskeleton; actin},
   language = {eng},
   location = {Praha},
   pages = {14-14},
   publisher = {Československá biologická společnost},
   title = {Detection of spectrin-like protein in yeasts: spectrin-actin relations},
   year = {2002}
}

TY  - JOUR
ID  - 406732
AU  - Slaninová, Iva - Holubářová, Alena - Svoboda, Augustin
PY  - 2002
TI  - Detection of spectrin-like protein in yeasts: spectrin-actin relations
PB  - Československá biologická společnost
CY  - Praha
KW  - yeast
KW  - spectrin
KW  - cytoskeleton
KW  - actin
N2  - Proteins related to the a- and b- spectrin have been detected in almost all vertebrate tissues, and also in plants (1) and hyphal fungae (2). We studied spectrin-like proteins in budding yeasts Saccharomyces cerevisiae and fission yeasts Schizosaccharomyces japonicus var. versatilis. Western blot analysis using anti-chicken and anti-human erythrocyte spectrin antibodies detected band at 220 kDa in cells and protoplasts extracts of both yeast strains. Additional bands were present at 50, 34 and 20 kDa in S. cerevisiae, and 44, 34 and 20 kDa in Sch. versatilis. Fluorescence microscopy revealed distinct labelling of the spectrin-like antigens at the cell surfaces and in vacuolar membranes of cells and protoplasts of both yeast species. In addition, the antigens were spread in the cytoplasm of both cells as fluorescent patches and as a diffuse fluorescence in Sch. versatilis nuclei. In mating and sporulating cells spectrin-like antigens lined the cell surface and prespore membranes. Electron microscopy of immunogold labelled cells and protoplasts revealed spectrin-like antigens in the cell wall, plasma membrane, ER, vacuole, nuclei, vesicles and mitochondria. Contrary to plant and some other fungal cells, spectrin in yeasts did not co-localize with actin. Growing regions of the cells were not preferentially labelled. Spectrin antigens correlated with actin only in protoplasts and maturing spores where patches of both lined the whole surface. The topology of spectrin was not affected by actin depolymerisation with Latrunculin B, nor was it changed in either act1-1 or cdc42 mutants under restrictive conditions. Under osmotic stress, both spectrin and actin were delocalized and appeared in the form of large clusters in the cytoplasm. Our results confirmed the role for spectrin as a membrane protein and the fact that spectrin localization occurs independently of actin distribution.
ER  -

SLANINOVÁ, Iva, Alena HOLUBÁŘOVÁ a Augustin SVOBODA. Detection of spectrin-like protein in yeasts: spectrin-actin relations. In \textit{Sborník X. Cytoskeletálního klubu}. Praha: Československá biologická společnost, 2002, s.~14.

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