Bioavailability and metabolism of artichoke caffeoylquinic
acids in man

D 2002

Bioavailability and metabolism of artichoke caffeoylquinic acids in man

SLANINA, Jiří a Eva TÁBORSKÁ

Základní údaje

Originální název

Bioavailability and metabolism of artichoke caffeoylquinic acids in man

Autoři

SLANINA, Jiří (203 Česká republika, garant) a Eva TÁBORSKÁ (203 Česká republika)

Vydání

Vysoké Tatry, Stará Lesná, Zborník XVIII. Biochemický zjazd, s. 250-250, 2002

Nakladatel

Slovenská společnost pre biochemiu a molekularnu biologiu pri SAV

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

30202 Endocrinology and metabolism

Stát vydavatele

Slovensko

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14110/02:00006752

Organizační jednotka

Lékařská fakulta

ISBN

nepřiděleno

Klíčová slova anglicky

bioavailability; artichoke; metabolism; caffeoylquinic acids; chlorogenic acid; caffeic acid; dihydrocaffeic acid; electrochemical detection; HPLC; antioxidants; Cynara; dicaffeoylquinic acid

Štítky

antioxidants, artichoke, bioavailability, caffeic acid, caffeoylquinic acids, chlorogenic acid, Cynara, dicaffeoylquinic acid, dihydrocaffeic acid, electrochemical detection, HPLC, metabolism

Změněno: 3. 12. 2002 10:38, Mgr. Jiří Slanina, Ph.D.

Anotace

V originále

Caffeoylquinic acids belong to the most frequently encountered phenolic acids in plants. They are present in many fruits (e.g. apples and pears) and vegetables (e.g. artichoke), chlorogenic acid (5-caffeoylquinic acid) occurs at very high level in coffee (CLIFFORD 2000). Caffeoylquinic acids display the strong antioxidant activity in respect of the presence of catechol moiety (PAULOVÁ et al., 2000). However, very little is known about their bioavailability and metabolism in humans. Analysis of urinary metabolites of artichoke caffeoylquinic acids using reversed-phase high-performance liquid chromatography is described. The HPLC system consisted of an LiChrospher 100 RP-18 column (5 um, 125 x 4 mm) and a mobile phase with 35% methanol in 50 mM phosphate buffer (pH 2.5). High selectivity and low detection limits of caffeic acid derivatives were achieved by using electrochemical detection (Coulochem II, ESA) at a low potential (channel 1: 0 mV, channel 2: 150 mV). The method was applied to the identification of caffeoylquinic acids metabolites in human urine after a single dose of an extract from the leaves of Cynara cardunculus taken orally. Conjugates were hydrolyzed enzymatically (glucuronidase/ sulfatase type HP-2, Sigma). In our study, caffeic acid and dihydrocaffeic acid were found in human urine after oral administration of Cynara extracts rich on chlorogenic acid (32 mg) and 1,5-dicaffeoylquinic acid (31 mg). Concentrations of caffeic acid and dihydrocaffeic acid in human urine increased after intake of the extract in the least 35 times and 14 times, respectively. Both phenolic acids were present in urine mostly as glucuronide and/or sulfate conjugates (approx. 95 %). However, 1,5-dicaffeoylquinic acid was not detected in urine and confirmation of presence of chlorogenic acid in urine involves next study.

Návaznosti

GA303/00/D062, projekt VaV

Název: Biologická dostupnost a mechanismus antioxidačního účinku fenolických kyselin a lignanů

Investor: Grantová agentura ČR, Biologická dostupnost a mechanismus antioxidačního účinku fenolických kyselin a lignanů

Citovat

SLANINA, Jiří a Eva TÁBORSKÁ. Bioavailability and metabolism of artichoke caffeoylquinic acids in man. In Zborník XVIII. Biochemický zjazd. Vysoké Tatry, Stará Lesná: Slovenská společnost pre biochemiu a molekularnu biologiu pri SAV, 2002, s. 250. ISBN nepřiděleno.

@inproceedings{406791,
   author = {Slanina, Jiří and Táborská, Eva},
   address = {Vysoké Tatry, Stará Lesná},
   booktitle = {Zborník XVIII. Biochemický zjazd},
   keywords = {bioavailability; artichoke; metabolism; caffeoylquinic acids; chlorogenic acid; caffeic acid; dihydrocaffeic acid; electrochemical detection; HPLC; antioxidants; Cynara; dicaffeoylquinic acid},
   language = {eng},
   location = {Vysoké Tatry, Stará Lesná},
   isbn = {nepřiděleno},
   pages = {250-250},
   publisher = {Slovenská společnost pre biochemiu a molekularnu biologiu pri SAV},
   title = {Bioavailability and metabolism of artichoke caffeoylquinic acids in man},
   year = {2002}
}

TY  - JOUR
ID  - 406791
AU  - Slanina, Jiří - Táborská, Eva
PY  - 2002
TI  - Bioavailability and metabolism of artichoke caffeoylquinic acids in man
PB  - Slovenská společnost pre biochemiu a molekularnu biologiu pri SAV
CY  - Vysoké Tatry, Stará Lesná
SN  - nepřiděleno
KW  - bioavailability
KW  - artichoke
KW  - metabolism
KW  - caffeoylquinic acids
KW  - chlorogenic acid
KW  - caffeic acid
KW  - dihydrocaffeic acid
KW  - electrochemical detection
KW  - HPLC
KW  - antioxidants
KW  - Cynara
KW  - dicaffeoylquinic acid
N2  - Caffeoylquinic acids belong to the most frequently encountered phenolic acids in plants. They are present in many fruits (e.g. apples and pears) and vegetables (e.g. artichoke), chlorogenic acid (5-caffeoylquinic acid) occurs at very high level in coffee (CLIFFORD 2000). Caffeoylquinic acids display the strong antioxidant activity in respect of the presence of catechol moiety (PAULOVÁ et al., 2000). However, very little is known about their bioavailability and metabolism in humans. Analysis of urinary metabolites of artichoke caffeoylquinic acids using reversed-phase high-performance liquid chromatography is described. The HPLC system consisted of an LiChrospher 100 RP-18 column (5 um, 125 x 4 mm) and a mobile phase with 35% methanol in 50 mM phosphate buffer (pH 2.5). High selectivity and low detection limits of caffeic acid derivatives were achieved by using electrochemical detection (Coulochem II, ESA) at a low potential (channel 1: 0 mV, channel 2: 150 mV). The method was applied to the identification of caffeoylquinic acids metabolites in human urine after a single dose of an extract from the leaves of Cynara cardunculus taken orally. Conjugates were hydrolyzed enzymatically (glucuronidase/ sulfatase type HP-2, Sigma). In our study, caffeic acid and dihydrocaffeic acid were found in human urine after oral administration of Cynara extracts rich on chlorogenic acid (32 mg) and 1,5-dicaffeoylquinic acid (31 mg). Concentrations of caffeic acid and dihydrocaffeic acid in human urine increased after intake of the extract in the least 35 times and 14 times, respectively. Both phenolic acids were present in urine mostly as glucuronide and/or sulfate conjugates (approx. 95 %). However, 1,5-dicaffeoylquinic acid was not detected in urine and confirmation of presence of chlorogenic acid in urine involves next study.
ER  -

SLANINA, Jiří a Eva TÁBORSKÁ. Bioavailability and metabolism of artichoke caffeoylquinic acids in man. In \textit{Zborník XVIII. Biochemický zjazd}. Vysoké Tatry, Stará Lesná: Slovenská společnost pre biochemiu a molekularnu biologiu pri SAV, 2002, s.~250. ISBN~nepřiděleno.

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