Computer-assisted quantitative immunohistochemistry of the
extracellular matrix in the dorsal and ventral spinal roots
with the use of a Lucia software

J 2002

Computer-assisted quantitative immunohistochemistry of the extracellular matrix in the dorsal and ventral spinal roots with the use of a Lucia software

DUBOVÝ, Petr; Ivana SVÍŽENSKÁ a Ilona KLUSÁKOVÁ

Základní údaje

Originální název

Computer-assisted quantitative immunohistochemistry of the extracellular matrix in the dorsal and ventral spinal roots with the use of a Lucia software

Autoři

DUBOVÝ, Petr; Ivana SVÍŽENSKÁ a Ilona KLUSÁKOVÁ

Vydání

Acta histochemica, Jena, Urban&Fischer Verlag, 2002, 0065-1281

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30000 3. Medical and Health Sciences

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 0.867

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/02:00006843

Organizační jednotka

Lékařská fakulta

UT WoS

000180301000011

Klíčová slova anglicky

endoneurium; extracellular; matrix; immunofluorescence; image analysis

Štítky

endoneurium, extracellular, Image analysis, immunofluorescence, matrix

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 26. 6. 2009 10:57, prof. RNDr. Petr Dubový, CSc.

Anotace

ORIG CZ

V originále

The endoneurial ECM is produced by Schwann cells and fibroblasts under the control of the axons. The dorsal and ventral spinal roots contain different types of axons, but no information is available about differences of their ECM content. The present paper demonstrates a comparison of immunofluorescence intensity for chondroitin sulfate proteoglycan, fibronectin, tenascin and thrombospondin in the endoneurial ECM of rat dorsal and ventral spinal roots. Sections through the dorsal and ventral roots were incubated simultaneously for indirect immunofluorescence detection of studied epitopes. The brightness of immunofluorescence staining was assessed by computer-assisted image analysis (Lucia G, Laboratory Imaging Ltd., Prague) using interactive segmentation of digitized pictures to select the areas of measurement. The results revealed quantitative differences of the endoneurial ECM content of the spinal dorsal and ventral roots probably corresponding to the presence of different types of axons. The ECM composition of the endoneurium in dorsal and ventral roots might be related to the formation of extrinsic conditions differently supporting regeneration of afferent and motor axons after their injury.

Česky

Endoneuriální ECM je produkována Schwannovými buňkami a fibroblasty pod kontrolou axonů. Složení endoneuriální ECM bylo studováno kvantitativní imunohistochemii.

Návaznosti

GA309/00/0407, projekt VaV

Název: Celulární a extracelulární prostředí pro regeneraci axonů při kořenových a distálních lezích periferních nervů

Investor: Grantová agentura ČR, Celulární a extracelulární prostředí pro regeneraci axonů při kořenových a distálních lezích periferních nervů

Citovat

DUBOVÝ, Petr; Ivana SVÍŽENSKÁ a Ilona KLUSÁKOVÁ. Computer-assisted quantitative immunohistochemistry of the extracellular matrix in the dorsal and ventral spinal roots with the use of a Lucia software. Acta histochemica. Jena: Urban&Fischer Verlag, 2002, roč. 104, č. 4, s. 371-374. ISSN 0065-1281.

@article{407044,
   author = {Dubový, Petr and Svíženská, Ivana and Klusáková, Ilona},
   article_location = {Jena},
   article_number = {4},
   keywords = {endoneurium; extracellular; matrix; immunofluorescence; image analysis},
   language = {eng},
   issn = {0065-1281},
   journal = {Acta histochemica},
   title = {Computer-assisted quantitative immunohistochemistry of the extracellular matrix in the dorsal and ventral spinal roots with the use of a Lucia software},
   volume = {104},
   year = {2002}
}

TY  - JOUR
ID  - 407044
AU  - Dubový, Petr - Svíženská, Ivana - Klusáková, Ilona
PY  - 2002
TI  - Computer-assisted quantitative immunohistochemistry of the extracellular matrix in the dorsal and ventral spinal roots with the use of a Lucia software
JF  - Acta histochemica
VL  - 104
IS  - 4
SP  - 371
EP  - 371
PB  - Urban&Fischer Verlag
SN  - 00651281
KW  - endoneurium
KW  - extracellular
KW  - matrix
KW  - immunofluorescence
KW  - image analysis
N2  - The endoneurial ECM is produced by Schwann cells and fibroblasts under the control of the axons. The dorsal and ventral spinal roots contain different types of axons, but no information is available about differences of their ECM content. The present paper demonstrates a comparison of immunofluorescence intensity for chondroitin sulfate proteoglycan, fibronectin, tenascin and thrombospondin in the endoneurial ECM of rat dorsal and ventral spinal roots. Sections through the dorsal and ventral roots were incubated simultaneously for indirect immunofluorescence detection of studied epitopes. The brightness of immunofluorescence staining was assessed by computer-assisted image analysis (Lucia G, Laboratory Imaging Ltd., Prague) using interactive segmentation of digitized pictures to select the areas of measurement. The results revealed quantitative differences of the endoneurial ECM content of the spinal dorsal and ventral roots probably corresponding to the presence of different types of axons. The ECM composition of the endoneurium in dorsal and ventral roots might be related to the formation of extrinsic conditions differently supporting regeneration of afferent and motor axons after their injury.
ER  -

DUBOVÝ, Petr; Ivana SVÍŽENSKÁ a Ilona KLUSÁKOVÁ. Computer-assisted quantitative immunohistochemistry of the extracellular matrix in the dorsal and ventral spinal roots with the use of a Lucia software. \textit{Acta histochemica}. Jena: Urban\&{}Fischer Verlag, 2002, roč.~104, č.~4, s.~371-374. ISSN~0065-1281.

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