FOJTOVÁ, Miloslava, Jana FULNEČKOVÁ, Jiří FAJKUS and Aleš KOVAŘÍK. Recovery of tobacco cells from cadmium stress is accompanied by DNA repair and increased telomerase activity. Journal of Experimental Botany. Oxford: OXFORD UNIV PRESS, 2002, roč. 53, No 378, p. 2151-2158. ISSN 0022-0957.
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Basic information
Original name Recovery of tobacco cells from cadmium stress is accompanied by DNA repair and increased telomerase activity
Authors FOJTOVÁ, Miloslava (203 Czech Republic), Jana FULNEČKOVÁ (203 Czech Republic), Jiří FAJKUS (203 Czech Republic, guarantor) and Aleš KOVAŘÍK (203 Czech Republic).
Edition Journal of Experimental Botany, Oxford, OXFORD UNIV PRESS, 2002, 0022-0957.
Other information
Original language English
Type of outcome Article in a journal
Field of Study Genetics and molecular biology
Country of publisher United Kingdom of Great Britain and Northern Ireland
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 2.852
RIV identification code RIV/00216224:14310/02:00006960
Organization unit Faculty of Science
UT WoS 000178957500003
Keywords in English apoptosis; cadmium; DNA domain fragmentation; telomerase; tobacco BY-2 cells
Tags apoptosis, cadmium, DNA domain fragmentation, Telomerase, tobacco BY-2 cells
Tags International impact, Reviewed
Changed by Changed by: prof. RNDr. Jiří Fajkus, CSc., učo 28574. Changed: 30/6/2009 12:49.
Abstract
It has been shown previously that apoptosis of tobacco cells induced by cadmium ions shows a relatively long lag period between exposure and ceel death. This lag phase lasts for 3 d in TBY-2 cell cultures and is characterized by the maintenance of full cell viability despite extensive fragmentation of DNA into pieces of chromatin loop size. Experiments reported here demonstrate that cell death can be prevented if CdSO4 is removed from the growth medium during the lag phase, suggesting that an irreversible apoptotic trigger is delivered within 24 h, between the third and fourth days of cadmium treatment. The post-cadmium recovery phase was characterized by DNA repair at the level of 50-200 kb and increased telomerase activity. Analysis of high-molecular-weight DNA by pulsed-field-gel electrophoresis revealed that the majority of DNA strand breaks was repaired within 48 h after cadmium withdrawal. Telomerase activity increased 2.5-fold in the recovery phase, but elevated levels were also found in cell extracts from apoptotic cells suggesting that telomerase might be associated with DNA repair, but it is not capable of inhibiting ongoing apoptosis. Limited exposure of TBY-2 cells to cadmium elicits non-random DNA damage of relatively high magnitude that can be repaired. It is proposed that plants might have developed a highly efficient DNA repair system to cope with transient genotoxic stress.
Links
MSM 143100008, plan (intention)Name: Genomy a jejich funkce
Investor: Ministry of Education, Youth and Sports of the CR, Genomes and their functions
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