Structural basis for oligosaccharide-mediated adhesion of
Pseudomonas aeruginosa in the lungs of cystic fibrosis patients

J 2002

Structural basis for oligosaccharide-mediated adhesion of Pseudomonas aeruginosa in the lungs of cystic fibrosis patients

MITCHELL, Edward; Corinne HOULES; Dvora SUDAKEVITZ; Michaela WIMMEROVÁ; Catherine GAUTIER et. al.

Základní údaje

Originální název

Structural basis for oligosaccharide-mediated adhesion of Pseudomonas aeruginosa in the lungs of cystic fibrosis patients

Autoři

MITCHELL, Edward (250 Francie); Corinne HOULES (250 Francie); Dvora SUDAKEVITZ (376 Izrael); Michaela WIMMEROVÁ (203 Česká republika, garant); Catherine GAUTIER (250 Francie); Serge PÉREZ (250 Francie); Albert M. WU (158 Tchaj-wan); Nechama GILBOA-GARBER (376 Izrael) a Anne IMBERTY (250 Francie)

Vydání

Nature Structural Biology, New York, Nature America Inc. 2002, 1072-8368

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 10.244

Kód RIV

RIV/00216224:14310/02:00007079

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

lectin; Pseudomonas aeruginosa; cystic fibrosis; crystal structure

Štítky

Crystal Structure, cystic fibrosis, lectin, Pseudomonas aeruginosa

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 4. 1. 2007 15:29, prof. RNDr. Michaela Wimmerová, Ph.D.

Anotace

V originále

Pseudomonas aeruginosa galactose- and fucose-binding lectins (PA-IL and PA-IIL) contribute to the virulence of this pathogenic bacterium, which is a major cause of morbidity and mortality in cystic fibrosis patients. The crystal structure of PA-IIL in complex with fucose reveals a tetrameric structure. Each monomer displays a nine-stranded, antiparallel b-sandwich arrangement and contains two close calcium cations that mediate the binding of fucose in a recognition mode unique among carbohydrate-protein interactions. Experimental binding studies, together with theoretical docking of fucose-containing oligosaccharides, are consistent with the assumption that antigens of the Lewis a (Lea) series may be the preferred ligands of this lectin. Precise knowledge of the lectin-binding site should allow a better design of new antibacterial-adhesion prophylactics.

Návaznosti

LN00A016, projekt VaV

Název: BIOMOLEKULÁRNÍ CENTRUM

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Biomolekulární centrum

Citovat

MITCHELL, Edward; Corinne HOULES; Dvora SUDAKEVITZ; Michaela WIMMEROVÁ; Catherine GAUTIER; Serge PÉREZ; Albert M. WU; Nechama GILBOA-GARBER a Anne IMBERTY. Structural basis for oligosaccharide-mediated adhesion of Pseudomonas aeruginosa in the lungs of cystic fibrosis patients. Nature Structural Biology. New York: Nature America Inc., 2002, roč. 9, č. 12, s. 918-921. ISSN 1072-8368.

@article{407796,
   author = {Mitchell, Edward and Houles, Corinne and Sudakevitz, Dvora and Wimmerová, Michaela and Gautier, Catherine and Pérez, Serge and Wu, Albert M. and GilboaandGarber, Nechama and Imberty, Anne},
   article_location = {New York},
   article_number = {12},
   keywords = {lectin; Pseudomonas aeruginosa; cystic fibrosis; crystal structure},
   language = {eng},
   issn = {1072-8368},
   journal = {Nature Structural Biology},
   title = {Structural basis for oligosaccharide-mediated adhesion of Pseudomonas aeruginosa in the lungs of cystic fibrosis patients},
   volume = {9},
   year = {2002}
}

TY  - JOUR
ID  - 407796
AU  - Mitchell, Edward - Houles, Corinne - Sudakevitz, Dvora - Wimmerová, Michaela - Gautier, Catherine - Pérez, Serge - Wu, Albert M. - Gilboa-Garber, Nechama - Imberty, Anne
PY  - 2002
TI  - Structural basis for oligosaccharide-mediated adhesion of Pseudomonas aeruginosa in the lungs of cystic fibrosis patients
JF  - Nature Structural Biology
VL  - 9
IS  - 12
SP  - 918
EP  - 918
PB  - Nature America Inc.
SN  - 10728368
KW  - lectin
KW  - Pseudomonas aeruginosa
KW  - cystic fibrosis
KW  - crystal structure
N2  - Pseudomonas aeruginosa galactose- and fucose-binding lectins (PA-IL and PA-IIL) contribute to the virulence of this pathogenic bacterium, which is a major cause of morbidity and mortality in cystic fibrosis patients. The crystal structure of PA-IIL in complex with fucose reveals a tetrameric structure. Each monomer displays a nine-stranded, antiparallel b-sandwich arrangement and contains two close calcium cations that mediate the binding of fucose in a recognition mode unique among carbohydrate-protein interactions. Experimental binding studies, together with theoretical docking of fucose-containing oligosaccharides, are consistent with the assumption that antigens of the Lewis a (Lea) series may be the preferred ligands of this lectin. Precise knowledge of the lectin-binding site should allow a better design of new antibacterial-adhesion prophylactics.
ER  -

MITCHELL, Edward; Corinne HOULES; Dvora SUDAKEVITZ; Michaela WIMMEROVÁ; Catherine GAUTIER; Serge PÉREZ; Albert M. WU; Nechama GILBOA-GARBER a Anne IMBERTY. Structural basis for oligosaccharide-mediated adhesion of Pseudomonas aeruginosa in the lungs of cystic fibrosis patients. \textit{Nature Structural Biology}. New York: Nature America Inc., 2002, roč.~9, č.~12, s.~918-921. ISSN~1072-8368.

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