Amperometric biosensors for detection of phenol using
chemically modified electrodes containing immobilized bacteria

J 2002

Amperometric biosensors for detection of phenol using chemically modified electrodes containing immobilized bacteria

SKLÁDAL, Petr, Natalya O. MOROZOVA a Anatoly N. RESHETILOV

Základní údaje

Originální název

Amperometric biosensors for detection of phenol using chemically modified electrodes containing immobilized bacteria

Autoři

SKLÁDAL, Petr (203 Česká republika, garant), Natalya O. MOROZOVA (643 Rusko) a Anatoly N. RESHETILOV (643 Rusko)

Vydání

Biosensors & Bioelectronics, Oxford, Elsevier Advanced Technology, 2002, 0956-5663

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.445

Kód RIV

RIV/00216224:14310/02:00007179

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000178267200005

Klíčová slova anglicky

Cell-based sensor;Mediated electron transfer;Screen-printed electrod;Phenols

Štítky

Cell-based sensor, Mediated electron transfer, Phenols, Screen-printed electrod

Změněno: 9. 1. 2003 13:57, prof. RNDr. Petr Skládal, CSc.

Anotace

V originále

Eight strains of Pseudomonas were studied for development of phenol sensor. The immobilization of cells was performed by absorbing them on the working part of mediator-modified screen-printed electrodes (SPEs). Only three Pseudomonas strains were able to transfer electrons resulting from specific oxidation of phenol to the electrode by means of mediators; ferrocene, duroquinone and dimethyferrocene were successfully used with the strains 394 (p20), 74-III and 83-IV (working names), respectively. The lower limits for detection of phenol were 1 muM for the strain 74-III and 10 muM for the strain 83-IV and 394 (p20). Calibrations were obtained as the dependencies of logarithm of current changes (log DeltaI) on logarithm of concentration (log C), log DeltaI vs. log C. Among all substrates tested (phenol, catechol, hydroquinone, ethanol, methanol, propanol, isopropanol, isobutanol, isoamylalcohol, acetate, glucose, xylose, vanillin, 2,4,6-trichlorphenol, 2,3,6-trichlorphenol, 4-hydroxy-3-methoxybenzoic acid, coumarin, pentafluorophenol), bacterial sensor demonstrated a good selectivity with respect to phenol and lower responses to catechol and hydroquinone (10-times lower). The dependence of signals on operating conditions was studied. The biosensor should be used during the day of preparation. The operational stability was satisfactory to perform Lip to 10 consecutive measurements. Low cost and very simple manufacturing procedure allow for bacterial sensor to be applied as disposable devices.

Návaznosti

OC 518.30, projekt VaV

Název: Sledování interakcí biomakromolekul s biovrstvami v různých konformačních stavech pomocí piezoelektrických biosensorů

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Sledování interakcí biomakromolekul s biovrstvami v různých konformačních stavech pomocí piezoelektrických biosensorů

Citovat

SKLÁDAL, Petr, Natalya O. MOROZOVA a Anatoly N. RESHETILOV. Amperometric biosensors for detection of phenol using chemically modified electrodes containing immobilized bacteria. Biosensors & Bioelectronics. Oxford: Elsevier Advanced Technology, 2002, roč. 17, č. 10, s. 867-873. ISSN 0956-5663.

@article{483146,
   author = {Skládal, Petr and Morozova, Natalya O. and Reshetilov, Anatoly N.},
   article_location = {Oxford},
   article_number = {10},
   keywords = {Cell-based sensor;Mediated electron transfer;Screen-printed electrod;Phenols},
   language = {eng},
   issn = {0956-5663},
   journal = {Biosensors & Bioelectronics},
   title = {Amperometric biosensors for detection of phenol using chemically modified electrodes containing immobilized bacteria},
   volume = {17},
   year = {2002}
}

TY  - JOUR
ID  - 483146
AU  - Skládal, Petr - Morozova, Natalya O. - Reshetilov, Anatoly N.
PY  - 2002
TI  - Amperometric biosensors for detection of phenol using chemically modified electrodes containing immobilized bacteria
JF  - Biosensors & Bioelectronics
VL  - 17
IS  - 10
SP  - 867
EP  - 867
PB  - Elsevier Advanced Technology
SN  - 09565663
KW  - Cell-based sensor;Mediated electron transfer;Screen-printed electrod;Phenols
N2  - Eight strains of Pseudomonas were studied for development of phenol sensor. The immobilization of cells was performed by absorbing them on the working part of mediator-modified screen-printed electrodes (SPEs). Only three Pseudomonas strains were able to transfer electrons resulting from specific oxidation of phenol to the electrode by means of mediators; ferrocene, duroquinone and dimethyferrocene were successfully used with the strains 394 (p20), 74-III and 83-IV (working names), respectively. The lower limits for detection of phenol were 1 muM for the strain 74-III and 10 muM for the strain 83-IV and 394 (p20). Calibrations were obtained as the dependencies of logarithm of current changes (log DeltaI) on logarithm of concentration (log C), log DeltaI vs. log C. Among all substrates tested (phenol, catechol, hydroquinone, ethanol, methanol, propanol, isopropanol, isobutanol, isoamylalcohol, acetate, glucose, xylose, vanillin, 2,4,6-trichlorphenol, 2,3,6-trichlorphenol, 4-hydroxy-3-methoxybenzoic acid, coumarin, pentafluorophenol), bacterial sensor demonstrated a good selectivity with respect to phenol and lower responses to catechol and hydroquinone (10-times lower). The dependence of signals on operating conditions was studied. The biosensor should be used during the day of preparation. The operational stability was satisfactory to perform Lip to 10 consecutive measurements. Low cost and very simple manufacturing procedure allow for bacterial sensor to be applied as disposable devices.
ER  -

SKLÁDAL, Petr, Natalya O. MOROZOVA a Anatoly N. RESHETILOV. Amperometric biosensors for detection of phenol using chemically modified electrodes containing immobilized bacteria. \textit{Biosensors \&{} Bioelectronics}. Oxford: Elsevier Advanced Technology, 2002, roč.~17, č.~10, s.~867-873. ISSN~0956-5663.

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