Quantitative MALDI TOFMS Analysis of Amino Acids Applying Soft Modeling Methods

MARŠÁLEK, Petr; Marta FARKOVÁ and Josef HAVEL. Quantitative MALDI TOFMS Analysis of Amino Acids Applying Soft Modeling Methods. Chemical Papers. Bratislava: Slovak Academic Press, 2002, vol. 56, No 3, p. 188-193. ISSN 0366-6352.

Basic information

Original name

Quantitative MALDI TOFMS Analysis of Amino Acids Applying Soft Modeling Methods

Authors

MARŠÁLEK, Petr; Marta FARKOVÁ and Josef HAVEL

Edition

Chemical Papers, Bratislava, Slovak Academic Press, 2002, 0366-6352

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Other information

Language

English

Type of outcome

Article in a journal

Field of Study

10406 Analytical chemistry

Country of publisher

Slovakia

Confidentiality degree

is not subject to a state or trade secret

Impact factor

Impact factor: 0.336

Organization unit

Faculty of Science

Tags

International impact, Reviewed

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Abstract

In the original language

This study demonstrates the applicability of Matrix Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOFMS) in multicomponent analysis of amino acids with various chemometric methods like experimental design, partial least squares (PLS), and artificial neural networks (ANN). Several negative factors influencing reproducibility of intensities were systematically studied and their influence was eliminated. Binary mixture of L-alanine and L-isoleucine were analyzed and evaluated using PLS and ANN. The results obtained show the possibility to use MALDI-TOFMS in quantitative analysis of amino acids.